Development of an effective extraction process for coenzyme Q10 from Artemia

Abstract

A method for extracting coenzyme Q10 (CoQ10) from Artemia was developed. 1 g of fresh Artemia was incubated with 75 % acetic acid at (30 ± 2)°C for 24 h, followed by three consecutive extractions with a mixture of 5 mL of hexane and 5 mL of ethanol, then analysis by a validated high-performance liquid chromatography with a diode-array detector. The calibration curve for CoQ10 was linear in a range of 1–50 μg mL−1. The limits of detection and quantification were 0.3 μg mL−1 and 1.1 μg mL−1, respectively. Mean recoveries were 94–100 % with a high precision of below 10 %. The method developed was found to be simple, efficient and the time required for releasing CoQ10 from Artemia was short. The method provides not only low energy consumption but is also practical for industrial applications.

Selective magnetic nanographene oxide solid‐phase extraction with high‐performance liquid chromatography and fluorescence detection for the determination of zearalenone in corn samples

“…The matrix effect can cause a positive or negative response of analyte , thus it was evaluated by comparing the slope of standard addition curve or matrix‐matched calibration curve performed by addition of standards into the extracted samples with the slope of standard curve for the ZEA concentration ranges of 0.1–5 mg/L. The study of matrix effect was fully described in the supporting Information Figure S1.…”

Robust colorimetric detection based on the anti-aggregation of gold nanoparticles for bromide in rice samples

“…In contrast to the aqueous standard solution, trace quantification of the analyte in real samples can suffer from the effects of the sample matrices, which can cause a positive or negative response by increasing background noise or suppressing the signal response. 49 Hence, the matrix effect was investigated using a standard addition curve ( Fig. 6b ) in the range 0.31–3.75 μM Br − (spiked into rice water extracts), which was compared to a standard calibration curve ( Fig.…”

Coenzyme Q10 and its Effective Sources

“…Liquid-liquid extraction or ultrasound extraction by using a mixture of hexane and 2-propanol found to be the most common methods for extraction of CoQ10 from different samples (Xue et al, 2012). For example, CoQ10 from fresh tobacco leaves and litchi pericarp was extracted using ultrasonic extraction in the presence of ethanol and hexane (Rujiralai et al, 2014).…”

“…For these reasons, some people rely on CoQ10 supplements. The daily intake of CoQ10 is suggested as 12 mg kg −1 (Rujiralai et al, 2014). More recently, nutraceutical supplements containing CoQ10 have gained a significant popularity in health management sections (Buettner et al, 2007).…”

“…As summarized in Table 3, CoQ10 can be produced by microbial fermentation including fungi (e.g., Candida, Sporidobolus, Rhodotorula, Neurospora, Aspergillus) and bacteria (e.g., Agrobacterium, Paracoccus, Cryptococcusi, Rhodobacter, Tricosporon). Moreover, presence of CoQ10 in Artemia samples as a Crustacean was investigated (Rujiralai et al, 2014). Microbial production offers an environmentally benign option based on the enzymatic catalysis at the cellular level for CoQ10 assembly.…”