Development of a validated RP-HPLC/DAD method for the quantitative determination of methyl jasmonate in an insect repellent semi-solid formulation

Abstract

A simple and efficient reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the first time for the estimation of a mosquito repellent, methyl jasmonate in a cream formulation, and validated as per the International Conference on Harmonization guidelines. Acetonitrile and water (75:25 v/v) were used as the mobile phase and the flow-rate of the mobile phase was kept constant at 1.0 mL/min. The analysis was performed isocratically on a C 18 analytical column (250 × 4.4 mm, 5 μm) using a Diode Array Detector for the detection of methyl jasmonate at 214 nm. The presence of excipients did not interfere with the quantification of methyl jasmonate. The calibration curve was linear in the concentration range of 25–300 μg/mL. The relative standard deviations for intra-day and inter-day precision, and repeatability were less than 2%. The recovery ranged from 88.5% to 90.7% with relative standard deviations not higher than 2%. The limit of detection and quantification were 9.4 μg/mL and 28.5 μg/mL, respectively. System suitability parameters were within the accepted range. The proposed method was also robust. Thus, the present report puts forward a novel analytical method for the estimation of an emerging mosquito repellent, methyl jasmonate by using the RP-HPLC technique.

Development, Validation, and Application of the UPLC-DAD Methodology for the Evaluation of the Qualitative and Quantitative Composition of Phenolic Compounds in the Fruit of American Cranberry (Vaccinium macrocarpon Aiton)

“…The accuracy of the method of the analysis is expressed in percentage and indicates the proximity between the measured value and the actual value of the analyte [51,52]. Accuracy should be assessed using a certified reference plant material or a matrix without the target analyte [53,54].…”

Nanoelicitors with prolonged retention and sustained release to produce beneficial compounds in wines

“…S1, ESI). 46 The loading capacity, i.e., adsorbed MeJ (mg) per mg of nanoMeJ (% wt), was calculated by quantifying the MeJ delivered from a known amount of nanoMeJ after 3 days of delivery (when the 100 % of release was achieved, Fig. 4).…”

A validated extraction technique followed by high‐performance liquid chromatography‐ultraviolet analysis for the assay of melittin as an indicator component of honey bee venom in cosmeceutical products

“…The linearity of a method essentially expresses how closely a linear regression of response versus analyte concentration follows a straight line [36]. Accordingly, the calibration curve was illustrated based on the respective area of 5 melittin concentrations.…”