Development of a Synthetic Medium for Rabbit Lens Culture in a Perfusion System

The Synthesis of Macromolecular 3‐Hydroxyproline by Attaching and Confluent Cultures of Human Fibroblasts

“…Ascorbate is known to be relatively unstable under the conditions utilized to culture cells in vitro [13]. The temporal variation in the ratio of 3-hydroxyproline/ 4-hydroxyproline observed in confluent cultures ( Table 2) might therefore reflect an effect of the decreasing concentration of ascorbate with time on the synthesis of the 3-hydroxy isomer.…”

The Synthesis of Macromolecular 3‐Hydroxyproline by Attaching and Confluent Cultures of Human Fibroblasts

“…Ascorbate is known to be relatively unstable under the conditions utilized to culture cells in vitro [13]. The temporal variation in the ratio of 3-hydroxyproline/ 4-hydroxyproline observed in confluent cultures ( Table 2) might therefore reflect an effect of the decreasing concentration of ascorbate with time on the synthesis of the 3-hydroxy isomer.…”

The Synthesis of Macromolecular 3‐Hydroxyproline by Attaching and Confluent Cultures of Human Fibroblasts

“…Ascorbate is known to be relatively unstable under the conditions utilized to culture cells in vitro [13]. The temporal variation in the ratio of 3-hydroxyproline/ 4-hydroxyproline observed in confluent cultures ( Table 2) might therefore reflect an effect of the decreasing concentration of ascorbate with time on the synthesis of the 3-hydroxy isomer.…”

The Synthesis of Macromolecular 3‐Hydroxyproline by Attaching and Confluent Cultures of Human Fibroblasts

“…Ascorbate is known to be relatively unstable under the conditions utilized to culture cells in vitro [13]. The temporal variation in the ratio of 3-hydroxyproline/ 4-hydroxyproline observed in confluent cultures ( Table 2) might therefore reflect an effect of the decreasing concentration of ascorbate with time on the synthesis of the 3-hydroxy isomer.…”