Development of a Specimen-Sparing Multichannel Bead Assay to Detect Antiparasite IgG4 for the Diagnosis of Schistosoma and Wuchereria Infections on the Coast of Kenya

DuVall, Adam; Fairley, Jessica K.; Sutherland, Laura J.; Bustinduy, Amaya L.; Mungai, Peter; Muchiri, Eric M.; Malhotra, Indu; Kitron, Uriel; King, Charles H.

doi:10.4269/ajtmh.13-0292

Cited by 13 publications

(12 citation statements)

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“…To increase detection of low-intensity infections, collected serum and plasma and red blood cells were stored at −80°C and later tested by enzyme-linked immunosorbent assay for evidence of circulating filarial OG4C3 antigen (TropBioMed, Townsville, Australia) and anti-Schistosoma soluble worm antigen proteins immunoglobulin G4, diagnostic of current or recent infection. 22 Red blood cell pellets underwent DNA extraction for polymerase chain reaction speciesspecific detection of malaria parasites. 23 …”

Section: Parasitological Diagnosismentioning

confidence: 99%

Pneumococcal Vaccine Response After Exposure to Parasites in Utero, in Infancy, or Mid-Childhood

et al. 2017

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BACKGROUND AND OBJECTIVE: Streptococcus pneumoniae is a leading cause of mortality before age 5, but few studies examine details of childhood response to pneumococcal vaccine in less-developed settings. Although malnutrition, HIV, and concurrent infections can impair response, evidence suggests that chronic parasitic infections can also contribute to poor vaccination results. The objective of this study was to determine whether response to pneumococcal vaccine varied among children either exposed to parasitic infections in utero, previously infected in infancy, or infected at the time of immunization.

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Section: Parasitological Diagnosismentioning

confidence: 99%

Pneumococcal Vaccine Response After Exposure to Parasites in Utero, in Infancy, or Mid-Childhood

et al. 2017

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“…Anti-Schistosoma IgG4 and anti-filarial IgG4, markers of active or very recent infection by these helminth parasites, were detected and quantified by specimen-sparing multiplex flow bead immunoassay as previously described. 22 Testing for anemia. Venous whole blood (4-5 mL) was collected from each subject by venipuncture.…”

Section: Methodsmentioning

confidence: 99%

Anemia Among Children Exposed to Polyparasitism in Coastal Kenya

Cojulun

Bustinduy

Sutherland

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. Anemia represents a substantial problem for children living in areas with limited resources and significant parasite burden. We performed a cross-sectional study of 254 Kenyan preschool-and early school-age children in a setting endemic for multiple chronic parasitic infections to explore mechanisms of their anemia. Complete venous blood cell counts revealed a high prevalence of local childhood anemia (79%). Evaluating the potential links between low hemoglobin and socioeconomic factors, nutritional status, hemoglobinopathy, and/or parasite infection, we identified age < 9 years (odds ratio [OR]: 12.0, 95% confidence interval [CI]: 4.4, 33) and the presence of asymptomatic malaria infection (OR: 6.8, 95% CI: 2.1, 22) as the strongest independent correlates of having anemia. A total of 130/155 (84%) of anemic children with iron studies had evidence of iron-deficiency anemia (IDA), 16% had non-IDA; 50/52 of additionally tested anemic children met soluble transferrin-receptor (sTfR) criteria for combined anemia of inflammation (AI) with IDA. Children in the youngest age group had the greatest odds of iron deficiency (OR: 10.0, 95% CI: 3.9, 26). Although older children aged 9-11 years had less anemia, they had more detectable malaria, Schistosoma infection, hookworm, and proportionately more non-IDA. Anemia in this setting appears multifactorial such that chronic inflammation and iron deficiency need to be addressed together as part of integrated management of childhood anemia.

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“…6). As IgG4 in humans decreases dramatically after treatment with praziquantel, detection of IgG4 antibody responses against helminthic infections can help differentiate active infection from prior exposure [60]. Thus, further study of human schistosomiasis will be required with the aim of detecting specific IgG4 to SmTR antigens in sera and urine, thereby providing more insights into these currently hypothetical proteins.…”

Section: Discussionmentioning

confidence: 99%

“…IgG4 subtypes in human and IgG1 in mouse are involved in resistance and susceptibility to schistosomiasis [58,59] and thus are of great interest for the diagnosis of helminthic diseases [60].…”

Section: Antibody Subtype Specific Reactionmentioning

confidence: 99%

Tandem repeat recombinant proteins as potential antigens for the sero-diagnosis of Schistosoma mansoni infection

Kalenda

Kato

Goto

et al. 2015

Parasitology International

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The diagnosis of schistosome infection, followed by effective treatment and/or mass drug administration, is crucial to reduce the disease burden. Suitable diagnostic tests and field-applicable tools are required to sustain schistosomiasis control programs. We therefore assessed the potential of tandem repeat (TR) proteins for serodiagnosis of Schistosoma mansoni infection using an experimental mouse model. TR genes in the genome of S. mansoni were searched in silico and 7 candidates, named SmTR1, 3, 8, 9, 10, 11 and 15, were selected. Total RNA was extracted from S. mansoni adult worms and eggs. Target TR genes were amplified, cloned, and the proteins were expressed in Escherichia coli competent cells. Female BALB/c mice were infected with 100 S. mansoni cercariae and sera were collected each week post-infection for 18 weeks. The levels of IgG antibodies to SmTR antigens were compared to those to soluble egg antigen (SEA) and to soluble worm antigen preparation (SWAP). Sera of infected mice reacted to all the antigens whereas those of naïve mice did not. IgG responses to SmTR1, 3, 9 and 10 were detected at the early stage of infection. Interestingly, antibodies reacting to SmTR3, 9, 10 and 15 dramatically decreased 4 weeks after treatment with praziquantel, while those against SEA and SWAP remained elevated. Our study suggests that TR proteins, especially SmTR10, may be suitable antigens for sero-diagnosis of infection by S. mansoni and are potential markers for monitoring and surveillance of schistosomiasis, including re-infection after treatment with praziquantel.

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Development of a Specimen-Sparing Multichannel Bead Assay to Detect Antiparasite IgG4 for the Diagnosis of Schistosoma and Wuchereria Infections on the Coast of Kenya

Cited by 13 publications

References 50 publications

Pneumococcal Vaccine Response After Exposure to Parasites in Utero, in Infancy, or Mid-Childhood

Pneumococcal Vaccine Response After Exposure to Parasites in Utero, in Infancy, or Mid-Childhood

Anemia Among Children Exposed to Polyparasitism in Coastal Kenya

Tandem repeat recombinant proteins as potential antigens for the sero-diagnosis of Schistosoma mansoni infection

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