2018
DOI: 10.3390/vetsci5030069
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Development of a Sensitive Real-Time Fast-qPCR Based on SYBR® Green for Detection and Quantification of Chicken Parvovirus (ChPV)

Abstract: Many viruses have been associated with runting and stunting syndrome (RSS). These viral infections mainly affect young chickens, causing apathy, depression, ruffled feathers, cloacal pasting, and diarrhea. Chicken Parvovirus (ChPV) is such an infection and has been detected in chickens showing signs of enteric diseases worldwide. Therefore, the present study aims to develop a sensitive real-time fast-qPCR assay based on SYBR® Green for detection and quantification of ChPV. A 561-bp non-structural (NS) gene was… Show more

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Cited by 10 publications
(16 citation statements)
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“…ChPV was detected initially in the intestinal content or in the intestinal wall [6,16,20,34], because the intestine is considered the primary target for enteric virus infection; however, other studies showed the presence of the virus in the spleen and pancreas [35], suggesting another tropism of enteric viruses. These results were supported by qPCR targeting the ChPV strain USP-362-3 [6]. Furthermore, the presence of ChPV was quantified and detected in the three segments of the small intestine (duodenum, jejunum and ileum) and increased after third day post-infection (p.i.)…”
Section: Discussionmentioning
confidence: 99%
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“…ChPV was detected initially in the intestinal content or in the intestinal wall [6,16,20,34], because the intestine is considered the primary target for enteric virus infection; however, other studies showed the presence of the virus in the spleen and pancreas [35], suggesting another tropism of enteric viruses. These results were supported by qPCR targeting the ChPV strain USP-362-3 [6]. Furthermore, the presence of ChPV was quantified and detected in the three segments of the small intestine (duodenum, jejunum and ileum) and increased after third day post-infection (p.i.)…”
Section: Discussionmentioning
confidence: 99%
“…Eighty (n = 80) day-old SPF chicks, supplied by CEVA (CEVA Animal Health, Campinas, Brazil), were divided into two groups of forty (n = 40) birds: group I was infected with 2 × 10 5 genome copies (GC) of ChPV in 200 µL of PBS, as previously determined through qPCR [6], and group II was mock infected with 200 µL of sterile 0.1 M PBS pH 7.4. All chicks were challenged by gavage.…”
Section: Experimental Infectionmentioning
confidence: 99%
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“…This method does not require the design of a separate probe, such as with the TaqMan qPCR technique, which can be complicated and expensive. Moreover, SYBR green qPCR appears to be more sensitive than the TaqMan probe-based qPCR [29,36]. The speci city of SYBR-green qPCR may also provide information regarding the amount of DNA ampli cation by using the melting curve analysis [37].…”
Section: Discussionmentioning
confidence: 99%