Development of a rapid detection and quantification method of Karenia mikimotoi by real-time quantitative PCR

Yuan, Jian Min; Mi, Tiezhu; Zhen, Yu; Yu, Zhigang

doi:10.1016/j.hal.2012.03.004

Cited by 66 publications

(22 citation statements)

References 34 publications

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“…This is similar to the LOD reported for other real-time PCR assays for Gymnodiniaceae species [50,51]. The amplification efficiency of all assays was between 93% and 106% (Table 3).…”

Section: Resultssupporting

confidence: 85%

Rapid and Accurate Identification by Real-Time PCR of Biotoxin-Producing Dinoflagellates from the Family Gymnodiniaceae

et al. 2014

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The identification of toxin-producing dinoflagellates for monitoring programmes and bio-compound discovery requires considerable taxonomic expertise. It can also be difficult to morphologically differentiate toxic and non-toxic species or strains. Various molecular methods have been used for dinoflagellate identification and detection, and this study describes the development of eight real-time polymerase chain reaction (PCR) assays targeting the large subunit ribosomal RNA (LSU rRNA) gene of species from the genera Gymnodinium, Karenia, Karlodinium, and Takayama. Assays proved to be highly specific and sensitive, and the assay for G. catenatum was further developed for quantification in response to a bloom in Manukau Harbour, New Zealand. The assay estimated cell densities from environmental samples as low as 0.07 cells per PCR reaction, which equated to three cells per litre. This assay not only enabled conclusive species identification but also detected the presence of cells below the limit of detection for light microscopy. This study demonstrates the usefulness of real-time PCR as a sensitive and rapid molecular technique for the detection and quantification of micro-algae from environmental samples.

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“…This is similar to the LOD reported for other real-time PCR assays for Gymnodiniaceae species [50,51]. The amplification efficiency of all assays was between 93% and 106% (Table 3).…”

Section: Resultssupporting

confidence: 85%

Rapid and Accurate Identification by Real-Time PCR of Biotoxin-Producing Dinoflagellates from the Family Gymnodiniaceae

et al. 2014

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“…Both ichthyotoxic compounds (Satake et al, 2005) and deoxygenation (Tangen, 1977) have been suggested to cause the observed mortalities although which of these is the main harmful agent remains uncertain. It is no surprise that extensive research is directed toward the detection (Shutler et al, 2012;Yuan et al, 2012) and, to a lesser extent, prediction of Karenia spp. blooms.…”

Section: à3mentioning

confidence: 99%

Drivers and effects of Karenia mikimotoi blooms in the western English Channel

Barnes

Tilstone

Smyth

et al. 2015

Progress in Oceanography

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“…These include mainly dinoflagellates and Raphidophyceae, but also Haptophyceae, Pelagophyceae, diatoms and Cyanophyceae. Representative genera among these groups are Alexandrium (Galluzzi et al 2004; Hosoi-Tanabe and Sako 2005; Dhyrman et al 2006; Erdner et al 2010; Garneau et al 2011), Karenia (Casper et al 2004; Kamikawa et al 2006; Yuan et al 2012), Cochlodinium , Heterocapsa , Chattonella , Heterosigma (Kamikawa et al 2006; Coyne et al 2005; Howard et al 2012), Prymnesium (Galluzzi et al 2008; Zamor et al 2012), Pseudo - nitzschia (Fitzpatrick et al 2010; Andrée et al 2011; Delanaey et al 2011), Dinophysis (Kavanagh et al 2010), Gambierdiscus (Vandersea et al 2012), and Ostreopsis (Perini et al 2011). …”

Section: Quantitative Real-time Pcr Applicationsmentioning

confidence: 99%

The quantitative real-time PCR applications in the monitoring of marine harmful algal bloom (HAB) species

Penna

Galluzzi

2012

Environ Sci Pollut Res

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In the last decade, various molecular methods (e.g., fluorescent hybridization assay, sandwich hybridization assay, automatized biosensor detection, real-time PCR assay) have been developed and implemented for accurate and specific identification and estimation of marine toxic microalgal species. This review focuses on the recent quantitative real-time PCR (qrt-PCR) technology developed for the control and monitoring of the most important taxonomic phytoplankton groups producing biotoxins with relevant negative impact on human health, the marine environment, and related economic activities. The high specificity and sensitivity of the qrt-PCR methods determined by the adequate choice of the genomic target gene, nucleic acid purification protocol, quantification through the standard curve, and type of chemical detection method make them highly efficient and therefore applicable to harmful algal bloom phenomena. Recent development of qrt-PCR-based assays using the target gene of toxins, such as saxitoxin compounds, has allowed more precise quantification of toxigenic species (i.e., Alexandrium catenella) abundance. These studies focus only on toxin-producing species in the marine environment. Therefore, qrt-PCR technology seems to offer the advantages of understanding the ecology of harmful algal bloom species and facilitating the management of their outbreaks.

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Development of a rapid detection and quantification method of Karenia mikimotoi by real-time quantitative PCR

Cited by 66 publications

References 34 publications

Rapid and Accurate Identification by Real-Time PCR of Biotoxin-Producing Dinoflagellates from the Family Gymnodiniaceae

Rapid and Accurate Identification by Real-Time PCR of Biotoxin-Producing Dinoflagellates from the Family Gymnodiniaceae

Drivers and effects of Karenia mikimotoi blooms in the western English Channel

The quantitative real-time PCR applications in the monitoring of marine harmful algal bloom (HAB) species

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