Development of a nuclear transformation system with a codon-optimized selection marker and reporter genes in Pyropia yezoensis (Rhodophyta)

Genome-Wide Identification and Functional Analysis of C2H2 Zinc Finger Transcription Factor Genes in the Intertidal Macroalga Pyropia haitanensis

“…At present, the gene editing and molecular verification methods of macroalgae are gradually developing, but the whole process is still in its primary stage [ 56 ]. The construction of the genetic system of Pyropia / Porphyra still faces many challenges, such as the complexity of the genome, the low efficiency of genetic transformation, the limitations of the selection marker system, and the difficulties in life cycle and reproductive characteristics [ 57 ].…”

Genome-Wide Identification and Functional Analysis of C2H2 Zinc Finger Transcription Factor Genes in the Intertidal Macroalga Pyropia haitanensis

“…At present, the gene editing and molecular verification methods of macroalgae are gradually developing, but the whole process is still in its primary stage [ 56 ]. The construction of the genetic system of Pyropia / Porphyra still faces many challenges, such as the complexity of the genome, the low efficiency of genetic transformation, the limitations of the selection marker system, and the difficulties in life cycle and reproductive characteristics [ 57 ].…”

RNA‐seq between asexual archeospores and meiosis‐related conchospores in Neopyropia yezoensis using Smart‐seq2

“…In fact, genetic transformation of reporter genes based on the characteristics of archeospore release of thalli in N. yezoensis has been reported (Hirata et al. 2014, Uji et al. 2014).…”

Isolation and characterization of a spore development mutant in Pyropia yezoensis (Rhodophyta) induced via insertional mutagenesis

“…The antibiotic resistance and reporter gene expression plasmid pEA7‐PyAct1: PyGUS (6812 bp) was prepared according to Hirata et al. ( 2014 ). Genetic transformation was performed using a particle delivery system, and 10‐mm square‐shaped pieces of WT gametophytes (approximately 3.5 × 10 5 cells) with archeosporangia were used as hosts.…”

Genome-Wide Identification and Functional Analysis of C2H2 Zinc Finger Transcription Factor Genes in the Intertidal Macroalga Pyropia haitanensis

“…At present, the gene editing and molecular verification methods of macroalgae are gradually developing, but the whole process is still in its primary stage [ 56 ]. The construction of the genetic system of Pyropia / Porphyra still faces many challenges, such as the complexity of the genome, the low efficiency of genetic transformation, the limitations of the selection marker system, and the difficulties in life cycle and reproductive characteristics [ 57 ].…”

RNA‐seq between asexual archeospores and meiosis‐related conchospores in Neopyropia yezoensis using Smart‐seq2

“…In fact, genetic transformation of reporter genes based on the characteristics of archeospore release of thalli in N. yezoensis has been reported (Hirata et al. 2014, Uji et al. 2014).…”

Isolation and characterization of a spore development mutant in Pyropia yezoensis (Rhodophyta) induced via insertional mutagenesis

“…The antibiotic resistance and reporter gene expression plasmid pEA7‐PyAct1: PyGUS (6812 bp) was prepared according to Hirata et al. ( 2014 ). Genetic transformation was performed using a particle delivery system, and 10‐mm square‐shaped pieces of WT gametophytes (approximately 3.5 × 10 5 cells) with archeosporangia were used as hosts.…”

Genome-Wide Identification and Functional Analysis of C2H2 Zinc Finger Transcription Factor Genes in the Intertidal Macroalga Pyropia haitanensis

“…At present, the gene editing and molecular verification methods of macroalgae are gradually developing, but the whole process is still in its primary stage [ 56 ]. The construction of the genetic system of Pyropia / Porphyra still faces many challenges, such as the complexity of the genome, the low efficiency of genetic transformation, the limitations of the selection marker system, and the difficulties in life cycle and reproductive characteristics [ 57 ].…”

RNA‐seq between asexual archeospores and meiosis‐related conchospores in Neopyropia yezoensis using Smart‐seq2

“…In fact, genetic transformation of reporter genes based on the characteristics of archeospore release of thalli in N. yezoensis has been reported (Hirata et al. 2014, Uji et al. 2014).…”

Isolation and characterization of a spore development mutant in Pyropia yezoensis (Rhodophyta) induced via insertional mutagenesis

“…The antibiotic resistance and reporter gene expression plasmid pEA7‐PyAct1: PyGUS (6812 bp) was prepared according to Hirata et al. ( 2014 ). Genetic transformation was performed using a particle delivery system, and 10‐mm square‐shaped pieces of WT gametophytes (approximately 3.5 × 10 5 cells) with archeosporangia were used as hosts.…”