Development of a novel set of microsatellite markers for Lippia alba (Verbenaceae)

Rocha, Daniel Sarto; Santos, Clesivan Pereira dos; Bajay, Miklos Maximiliano; Campos, Jaqueline Bueno de; Blank, Arie Fitzgerald; Pinheiro, José Baldin; Zucchi, Maria Imaculada

doi:10.4238/2015.february.3.4

Cited by 9 publications

(5 citation statements)

References 6 publications

(5 reference statements)

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“…Six of them produced polymorphisms, dividing the germplasm into two clusters. The data assists the characterization of germplasm banks, L. alba breeding programs, and other studies on genetic diversity and classification of species of the genus Lippia [64]. SSR markers were also developed for Phyla scaberrima to determine the genetic variability and population structure patterns of the species, totaling 11 primers.…”

Section: Genetic Variability Analysis Was Carried Out Between Populations Of Minthostachys Verticillata Collected In Central Andmentioning

confidence: 99%

Molecular Analyses used in Genetic Diversity Studies of Bioactive Plants of Verbenaceae and Lamiaceae: A Review

Blank

2018

ARR

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Morphological traits used to be employed to differentiate plant genotypes within breeding programs. With the advancement of biotechnology, biochemical markers and DNA molecular markers have become the primary tools used in the differentiation of individuals, because these markers are not influenced by the environment, as it occurs with morphological traits. Consequently, these new techniques provide faster and more precise results. The isoenzymes and the DNA molecular markers RFLP, RAPD, AFLP, and SSR stand out among the biochemical and molecular markers. Each molecular marker presents advantages and limitations, which increases the applicability to different species and laboratory conditions, presenting more reliable results when used in association. Given the increase in the number of works related to bioactive plant species, this review seeks to present the panorama of the use of biochemical and molecular markers in studies on the genetic diversity involving the families Verbenaceae and Lamiaceae, which encompass a large number of medicinal and aromatic species of economic importance.

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Section: Genetic Variability Analysis Was Carried Out Between Populations Of Minthostachys Verticillata Collected In Central Andmentioning

confidence: 99%

Molecular Analyses used in Genetic Diversity Studies of Bioactive Plants of Verbenaceae and Lamiaceae: A Review

Blank

2018

ARR

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“…Microsatellite primers were developed in Brazil and optimized for Lippia alba to characterize the L. alba germplasm by Rocha et al (2015). The data provide support to characterize germplasm banks, genetic breeding programs for L. alba, and other genetic diversity studies and classifications of species in the genus Lippia.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and geographical genetic diversity in Colombian accessions of Lippia alba (Mill.) N.E. Brown

Montoya

Muñoz

2019

rcia

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Bushy matgrass (Lippia alba, Verbenaceae) is a promising plant genetic resource, by their active compounds. The present document studied the molecular genetic diversity and spatial genetic structure of two contrasting populations of L. alba in Colombia. Eight RAM, evaluated total DNA of 59 accessions of non-cultivated plants collected in two Colombia regions, Chicamocha and Sumapaz. The expected average heterozygosity (or average heterozygosity genetic diversity of Nei) for the sample had low value (0.0≤He=0.2467≤0.5). The values of molecular diversity (MD) indicated values in the range of 0.1219 to 0.3425 for seven RAM. The frequency of variants is based on an effective number of alleles [Ae] and expected heterozygosity [He], genetic diversity by locus (hj=1-p2-q2) had maximum values (near 0.5) in the primers ACA, AG, CGA, and CEC. RAM suitably analyzed Lippia alba as an endemic genetic resource. A DNA bank composed of 59 Colombian accessions from Lippia alba was set up. The analysis of the spatial global structure shows that the subpopulation Sumapaz is structured, whilst the subpopulation Chicamocha, is in the structuring process. The results suggest in all cases the need for implementing: a) exchange of gene-seed, (b) gene banks with maximum genetic variability and c) induce genetic diversity.

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“…In aromatic species of medicinal interest, studies of genetic structure have concentrated on germplasm of domesticated species or those which chemotypes have already been identified and have consolidated space in the market. Among the species studied are lemon balm (Lippia alba), pepper rosemary (Lippia sidoides) and Mexican oregano (Lippia origanoides Hunth and Lippia graveolens) (Manica-Cattani, Zacaria, Pauletti, Atti-Serafini, & Echeverrigaray, 2009;Vega-Vela & Sánchez, 2012;Rocha et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of <i>Lippia rotundifolia</i> Cham. in Minas Gerais, Brazil

Meira¹,

Martins²,

Resende³

2019

Acta Sci. Biol. Sci.

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Lippia rotundifolia is an aromatic species, endemic from Campos rupestres and isolated by mountains. We aimed with the research to study the genetic variability of chá-de-pedestre (Lippia rotundifolia Cham.) with natural occurrence in ten places of the State of Minas Gerais, from molecular markers of ISSR type. The places of collection were: Parque Estadual de Serra Nova; Parque Estadual Veredas do Peruaçu; Abóboras community; Gigante community; edge of Rio do Peixe; environmental preservation area of Olhos d´água; private property in Joaquim Felício; Parque Estadual do Rio Preto; São Gonçalo do Rio das Pedras and brook of Rio Tigre. After the establishment of the extraction and amplification of DNA fragments with primers ISSR, an array of genetic distance was built. From this matrix, we analyzed the genetic diversity index as the allelic frequency (Na and Ne), Shannos´s index (H´), polymorphism (PLP), heterozygosity (He) and gene flow (Nm). The result showed higher genetic variability within the population (93%). The genetic diversity index was low (He = 0.132; H´= 0.214; Na = 1.111; Ne = 1.183; PLP = 56.67%). In general the species have low genetic diversity. The greatest diversity in populations occurred with an average temperature of 20°C. The Rio Tigre showed higher genetic distance and isolation.

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Development of a novel set of microsatellite markers for Lippia alba (Verbenaceae)

Cited by 9 publications

References 6 publications

Molecular Analyses used in Genetic Diversity Studies of Bioactive Plants of Verbenaceae and Lamiaceae: A Review

Molecular Analyses used in Genetic Diversity Studies of Bioactive Plants of Verbenaceae and Lamiaceae: A Review

Genetic diversity and geographical genetic diversity in Colombian accessions of Lippia alba (Mill.) N.E. Brown

Genetic diversity of <i>Lippia rotundifolia</i> Cham. in Minas Gerais, Brazil

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