Development of a new contact lens multipurpose solution: Comparative analysis of microbiological, biological and clinical performance

Kilvington, Simon; Li-min, Huang; Kao, Eugenia; Powell, Charles H.

doi:10.1016/s1888-4296(10)70019-4

Cited by 33 publications

(31 citation statements)

References 33 publications

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“…For example, MPDS-4 and a solution similar to MPDS-2 (based on PQ-1 and MAPD) were reported to give >2 to ‡3 log 10 kill with trophozoites of A. castellanii and A. polyphaga strains after a 6-hour exposure, which is comparable to the findings of this study. 20,25,29,31 Similarly, the one-step hydrogen peroxide system (PER-1) gave >3 log 10 kill of trophozoites with strains of A. castellanii (including 50370) and A. polyphaga. 21,29,31 In another study no difference in the degree of disinfection by PHMB-based MPDS was observed with two A. castellanii strains (including 50370) cultured monoxenically or axenically.…”

Section: Discussionmentioning

confidence: 99%

“…19,20 Alternative methods use 3% hydrogen peroxide with a neutralization step, typically a platinum-coated disc inside the storage case (one-step system) or following the addition of a separate catalase enzyme tablet (two-step system). 21,22 As a requirement for a product to be marketed as a contact lens disinfectant, it must comply with the international standard ISO 14729, which requires the demonstration of antimicrobial activity against specified bacteria, yeast, and mold within the manufacturer's recommended disinfection time.…”

mentioning

confidence: 99%

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Development of Standardized Methods for Assessing Biocidal Efficacy of Contact Lens Care Solutions AgainstAcanthamoebaTrophozoites and Cysts

Kilvington

Lam

2013

Invest. Ophthalmol. Vis. Sci.

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Citation: Kilvington S, Lam A. Development of standardized methods for assessing biocidal efficacy of contact lens care solutions against Acanthamoeba tophozoites and cysts. Invest Ophthalmol Vis Sci. 2013;54:4527-4537. DOI:10.1167/iovs.13-11927 PURPOSE. To investigate experimental variables in the development of standardized methods to assess the efficacy of contact lens disinfection systems against the trophozoite and cysts of Acanthamoeba spp. METHODS.A. castellanii (ATCC 50370), A. polyphaga (ATCC 30461), and A. hatchetti (CDC: V573) were adapted to axenic culture and used to produce cysts either with Neff's encystment medium (NEM) or starvation on nonnutrient agar (NNA). Challenge test assays and a most probable number approach were used to compare the trophozoite and cysticidal efficacy of four multipurpose disinfectant solutions (MPDSs) and a one-step hydrogen peroxide system (with and without the neutralizing step). RESULTS.With trophozoites, four of four MPDSs and the one-step peroxide system gave ‡3 log 10 kill for all strains 6 hours, regardless of culture medium used. Greater resistance was found against cysts, with results for MPDSs varying by species and method of cyst production. Here, 1-3 log 10 kill was found with NEM cysts for three of four MPDSs compared with one of four for the NNA cysts at 6 hours (A. castellanii and A. polyphaga, only). The one-step peroxide system gave 1-1.9 log 10 kill with NEM cysts and 0.8-1.1 for NNA cysts. Only 3% hydrogen peroxide gave total kill (>3 log 10 ) of NNA cysts at 6 hours. CONCLUSIONS.A reproducible method for determining the susceptibility of Acanthamoeba trophozoites and cysts to contact lens care systems has been developed. This will facilitate assay standardization for assessing the efficacy of such products against the organism and aid development of improved disinfectant and therapeutic agents.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Development of Standardized Methods for Assessing Biocidal Efficacy of Contact Lens Care Solutions AgainstAcanthamoebaTrophozoites and Cysts

Kilvington

Lam

2013

Invest. Ophthalmol. Vis. Sci.

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“…MPS formulations associated with increased hyperfluorescence on the ocular surface (P-PQ1-1107 and P-1107)(Jones et al 2002;Andrasko & Ryen 2008; Tilia et al 2012) both cause in cells. Conversely PQ1-Alex-904 or PQ1-Aldox-1304 resulted in little or no fluorescein staining, also as is found clinically(Kilvington et al 2010; Tilia et al 2012). The interaction of a contact lens material itself with the MPS may contribute to the hyperfluorescence observed clinically.However our finding that the clinically observed effects of these four MPS can be reproduced without the presence of a lens, suggests that SICS may primarily involve the biochemical effects of the MPS alone.Non-viable necrotic cells (following BKC treatment) are unable to take up fluorescein, consistent with our previous findings(Bakkar et al 2014) and with those of Bandamwar et al who reported minimal fluorescein fluorescence in non-viable human corneal limbal epithelial (HCLE) cells (Bandamwar et al 2014).Interestingly MPS formulations P-PQ1-1107 and P-1107, both of which are associated with clinical hyperfluorescence, produced limited or no increased PI staining respectively and no significant decreases in metabolic activity.…”

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confidence: 63%

Cellular fluorescein hyperfluorescence is dynamin-dependent and increased by Tetronic 1107 treatment

Khan

Price

Morgan

et al. 2018

The International Journal of Biochemistry & Cell Biology

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Sodium fluorescein ('fluorescein') staining of the ocular surface is frequently an indicator of compromised ocular health, and increases in the presence of certain contact lens multi-purpose solutions (MPS), a phenomenon known as solution induced corneal staining (SICS). The mechanism(s) underpinning fluorescein hyperfluorescence are uncertain, though may reflect increased cellular uptake of fluorescein by corneal epithelial cells. We have developed an in vitro model to study fluorescein uptake in both 'generic' mammalian cells (murine fibroblasts) and human corneal cells. Fluorescein hyperfluorescence increased after treatment with two MPS associated with clinical corneal fluorescein staining, yet there was no cellular hyperfluorescence for two MPS that do not cause this staining. Increased fluorescein uptake did not correlate with presence of a necrotic or an apoptotic marker (propidium iodide and caspase-3 respectively). Incubation of MPS-treated cells with dynasore (an inhibitor of dynamin, implicated in endocytic pathways) reduced fluorescein uptake irrespective of MPS treatment. The non-ionic surfactant Tetronic 1107 (present in both MPS associated with corneal fluorescein staining) increased uptake of fluorescein for both cell types, whereas an unrelated surfactant (Triton X-100) did not. We conclude that the clinical hyperfluorescence profile observed after exposure to four MPS can be reproduced using a simple model of cellular fluorescein uptake, suggesting this is the biological basis for SICS. Fluorescein entry does not correlate with necrosis or apoptosis, but instead involves a dynamin-dependent active process. Moreover the surfactant Tetronic 1107 appears to be a key MPS constituent triggering increased fluorescein entry, and may be the major factor responsible for SICS.

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“…5,9,12 Also, although the measured amount of sorbed lipid varies widely from study to study, our observed lower sorption of both CH and DPPC on lotrafilcon B relative to other SiHy lenses is directionally consistent with a number of other in vitro studies, as well as a clinical study showing lower lipid sorption to that lens. 12,13,28 For qualitative studies of lipid sorption, the chemical characteristics of the lens may be more important than the particulars of the in vitro model or the absolute amounts of sorbed lipid measured.…”

Section: Clinical Applicabilitymentioning

confidence: 99%

Quantitation of cholesterol and phospholipid sorption on silicone hydrogel contact lenses

et al. 2013

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The introduction of silicone hydrogel (SiHy) contact lenses to the consumer marketplace necessitates study of the susceptibility of these lenses to spontaneous deposition by hydrophobic lipid components of ocular tears. The use of radioisotopes to measure lipid sorption on SiHy contact lenses gives precise and accurate results but requires institutional infrastructure and compels efficient lipid removal from the lens. This study compares three methods of quantitating phospholipid and cholesterol sorption on SiHy lenses using radiolabeled cholesterol and phosphatidylcholine that were sorbed on lenses from an artificial tear fluid. A triple extraction technique using n-propanol gives the most reliable results. Comparison of sorption on SiHy lenses shows that balafilcon A and senofilcon A lenses sorb similar amounts, while lotrafilcon B lenses sorb comparatively less.

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Development of a new contact lens multipurpose solution: Comparative analysis of microbiological, biological and clinical performance

Cited by 33 publications

References 33 publications

Development of Standardized Methods for Assessing Biocidal Efficacy of Contact Lens Care Solutions AgainstAcanthamoebaTrophozoites and Cysts

Development of Standardized Methods for Assessing Biocidal Efficacy of Contact Lens Care Solutions AgainstAcanthamoebaTrophozoites and Cysts

Cellular fluorescein hyperfluorescence is dynamin-dependent and increased by Tetronic 1107 treatment

Quantitation of cholesterol and phospholipid sorption on silicone hydrogel contact lenses

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