Development of a Multifunctional Platform Based on Strong, Intrinsically Photoluminescent and Antimicrobial Silica‐Poly(citrates)‐Based Hybrid Biodegradable Elastomers for Bone Regeneration

Abstract

Biodegradable biomaterials with intrinsically multifunctional properties such as high strength, photoluminescent ability (bioimaging monitoring), and antimicrobial activity (anti‐infection), as well as high osteoblastic differentiation ability, play a critical role in successful bone tissue regeneration. However, fabricating a biomaterial containing all these functions is still a challenge. Here, urethane cross‐linked intrinsically multifunctional silica‐poly(citrate) (CMSPC)‐based hybrid elastomers are developed by first one‐step polymerization and further chemical crosslinked using isocyanate. CMSPC hybrid elastomers demonstrate a high modulus of 976 ± 15 MPa, which is superior compared with most conventional poly(citrate)‐based elastomers. Hybrid elastomers show strong and stable intrinsic photoluminescent ability (emission 400–600 nm) due to the incorporation of silica phase. All elastomers exhibit high inherent antibacterial properties against Staphylococcus aureus. In addition, CMSPC hybrid elastomers significantly enhance the proliferation and metabolic activity of osteoblasts (MC3T3‐E1). CMSPC hybrid elastomers significantly promote the osteogenic differentiation of MC3T3‐E1 by improving alkaline phosphatase activity and calcium biomineralization deposits, as well as expressions of osteoblastic genes. These hybrid elastomers also show a minimal inflammatory response indicated by subcutaneous transplantation in vivo. These optimized structure and multifunctional properties make this hybrid elastomer highly promising for bone tissue regeneration and antiinfection and bioimaging applications.

Biodegradable Multifunctional Bioactive Glass-Based Nanocomposite Elastomers with Controlled Biomineralization Activity, Real-Time Bioimaging Tracking, and Decreased Inflammatory Response

“…The characteristic bands at 1100–1200 cm –1 mainly represent the stretching vibration of the C–N bond, which is due to the grafted siloxane in the PCS. The disappearance of characteristic peaks of C–N (2300–2500 cm –1 ) and the presence of characteristic peaks of N–H at 3200–3400 cm –1 and amide at 1550–1650 cm –1 also showed the success in cross-linking by HDI . The peak at 1050–1100 cm –1 was identified as the absorption of Si–O–Si, confirming the formation of a silica network in the PCS matrix (Figure e) .…”

“…The disappearance of characteristic peaks of C−N (2300−2500 cm −1 ) and the presence of characteristic peaks of N−H at 3200−3400 cm −1 and amide at 1550−1650 cm −1 also showed the success in crosslinking by HDI. 31 The peak at 1050−1100 cm −1 was identified as the absorption of Si−O−Si, confirming the formation of a silica network in the PCS matrix (Figure 1e). 31 These results suggested that the amorphous PCS−BGN nanocomposites were fabricated through the HDI cross-linking method under low temperature.…”

“…31 The peak at 1050−1100 cm −1 was identified as the absorption of Si−O−Si, confirming the formation of a silica network in the PCS matrix (Figure 1e). 31 These results suggested that the amorphous PCS−BGN nanocomposites were fabricated through the HDI cross-linking method under low temperature.…”

Multifunctional Bioactive Dual‐Layered Nanofibrous Matrix for Effective Breast Cancer Therapy and Enhanced Wound Healing

“…The peaks in the range of 2.670–2.838 ppm (─CH₂─ in peak d) were attributed to citric acid. [ 12a ] The molecular weight (MW) of POC, determined by gel permeation chromatography (GPC), was 32.3 kDa. To optimize the composition of the MBDL‐NanoMat structure for future biomedical applications, various concentrations of PCL (3%, 5%, 7%, and 10% (w/v)) and gelatin (0%, 3%, 5%, and 7% (w/v)) were blended in different ratios to achieve a total concentration of 10% (w/v).…”

“…To enhance the tensile strength, POC (0.2, 0.5, and 1% (w/v)) was added due to its elastomeric properties, which have been used in tissue regeneration, bioimaging, and gene delivery. [ 6 , 12 ] Tensile strength increased with the addition of POC but declined sharply at 1%. In contrast, elongation increased up to 0.2% POC but significantly decreased beyond that point (Figure S3 , Supporting Information).…”