Development of a microsphere‐based immunoassay for the serological detection of glanders in equids

Laroucau, Karine; Saqib, Muhammad; Martin, Brigitte E.; Deshayes, Thomas; Bertin, Claire; Wernery, Ulrich; Joseph, Sunitha; Singha, Harisankar; Tripathi, Bhupendra Nath; Beck, Cécile

doi:10.1016/j.actatropica.2020.105463

Cited by 8 publications

(17 citation statements)

References 16 publications

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“…Sera from six horses were collected and tested for glanders, and five gave conclusive positive results using the OIE reference CFT (Table 1). The results were confirmed using two more serological tests: the iELISA ID Screen ® Glanders indirect test (IDvet, France) (Elschner et al., 2019) and a new Luminex ® bead‐based assay targeting four recombinant proteins of B. mallei (BimA, Hcp1, GroEL and TssB) as previously described by us (Laroucau et al, 2020). Both methods confirmed the seropositivity of five horses for B. mallei (Table 1).…”

Section: Horse Id Cft Ielisa Luminex® Results Titre Results % Pos Bim...mentioning

confidence: 55%

A genetic variant of Burkholderia mallei detected in Kuwait: Consequences for the PCR diagnosis of glanders

Laroucau

Aaziz

Vorimore

et al. 2020

Transbounding Emerging Dis

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Glanders is a contagious zoonotic disease caused by Burkholderia mallei. Following the detection of glanders positive horses using the OIE complement fixation test, the tissues of two horses were analysed by PCR. While PCR systems targeting the Burkholderia pseudomallei complex gave positive signals, the species-specific PCR systems targeting B. mallei (fliP-IS407A) and B. pseudomallei (orf11)-the OIE recommended targets-resulted in negative signals. However, the presence of B. mallei in these tissues was confirmed with a recently described B. mallei-specific real-time PCR system and genotyping with MLST-and SNP-based methods, performed on the most positive tissue, identified a genotype closely related to B. mallei strains recently isolated in the Middle East. This study leads to recommendations regarding the use of PCR systems for the molecular diagnosis of glanders, especially in regions where the circulating B. mallei strains have not yet been fully genetically characterized.

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Section: Horse Id Cft Ielisa Luminex® Results Titre Results % Pos Bim...mentioning

confidence: 55%

A genetic variant of Burkholderia mallei detected in Kuwait: Consequences for the PCR diagnosis of glanders

Laroucau

Aaziz

Vorimore

et al. 2020

Transbounding Emerging Dis

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“…In addition, several equine diseases are monitored through the reference laboratories’ network for international horse trade, which requires negative diagnostic results to allow the movement of horses. The technique’s multiplexing capability could be used to extend the diagnostic scope of this test to other equine diseases by combining, for example, the different tests already developed 30 – 32 within a single multiplex test.…”

Section: Discussionmentioning

confidence: 99%

Development of a microsphere-based immunoassay for the serological diagnosis of equine trypanosomosis

Verney

Gautron

Lemans

et al. 2022

Sci Rep

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Trypanozoon infections in equids are caused by three parasite species in the Trypanozoon subgenus: Trypanosoma equiperdum, T. brucei and T. evansi. They are respectively responsible for infectious diseases dourine, nagana and surra. Due to the threat that Trypanozoon infection represents for international horse trading, accurate diagnostic tests are crucial. Current tests suffer from poor sensitivity and specificity, due in the first case to the transient presence of parasites in the blood and in the second, to antigenic cross-reactivity among Trypanozoon subspecies. This study was designed to develop a microsphere‐based immunoassay for diagnosing equine trypanosomosis. We tested beads coated with eight Trypanosoma spp. recombinant antigens: enolase, GM6, PFR1, PFR2, ISG65, VSGat, RoTat1.2 and JN2118HU. Of these, GM6 was identified as the best candidate for the serological diagnosis of Trypanozoon infections in equids. Using a receiver operating characteristic (ROC) analysis on 349 equine sera, anti-GM6 antibodies were detected with an AUC value of 0.994 offering a sensitivity of 97.9% and a specificity of 96.0%. Our findings show that the GM6 antigen is a good target for diagnosing equine trypanosomosis using a microsphere‐based immunoassay. This promising assay could be a useful alternative to the official diagnostic tool for equine trypanosomosis.

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“…The anticomplementary activity of equine sera and false-positive CFT results are further limitations ( 14 , 15 ). Alternative tests (Western blot, enzyme-linked immunosorbent assays [ELISAs], Luminex) have been developed, and recent validation works give hope for improving the glanders diagnostic performance ( 16 – 18 ). In particular, the use of recombinant proteins (including Hcp1 and GroEL) has recently led to promising prospects for glanders diagnosis ( 17 , 19 , 20 ).…”

Section: Introductionmentioning

confidence: 99%

“…Alternative tests (Western blot, enzyme-linked immunosorbent assays [ELISAs], Luminex) have been developed, and recent validation works give hope for improving the glanders diagnostic performance ( 16 – 18 ). In particular, the use of recombinant proteins (including Hcp1 and GroEL) has recently led to promising prospects for glanders diagnosis ( 17 , 19 , 20 ). However, additional antigens might be useful to increase the performance of serodiagnostic glanders tests and distinguish between exposure and disease, acute and chronic forms, or melioidosis and glanders.…”

Section: Introductionmentioning

confidence: 99%

Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics

et al. 2023

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Development of a microsphere‐based immunoassay for the serological detection of glanders in equids

Cited by 8 publications

References 16 publications

A genetic variant of Burkholderia mallei detected in Kuwait: Consequences for the PCR diagnosis of glanders

A genetic variant of Burkholderia mallei detected in Kuwait: Consequences for the PCR diagnosis of glanders

Development of a microsphere-based immunoassay for the serological diagnosis of equine trypanosomosis

Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics

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