Development of a microparticle-enhanced nephelometric immunoassay for quantitation of human lysozyme in pleural effusion and plasma

Pleural fluid lysozyme as a diagnostic biomarker of pleural tuberculosis

“…Our literature search yielded 89 citations [ Figure 1 ], of which we ultimately included 11 studies reporting information on diagnostic accuracy ( N = 7) or comparing LP data between tuberculous and other effusions ( N = 10). [ 22 23 24 25 26 27 28 29 31 32 ] The major attributes of these 11 publications are summarized in Table 1 . The maximum number of studies (5, 45.5%) were conducted in Spain.…”

“…The maximum number of studies (5, 45.5%) were conducted in Spain. [ 22 24 30 31 32 ] Only two studies (18.2%), both from India, were conducted in a country with a high burden of TB. [ 27 29 ] One Indian study reported data exclusively from pediatric subjects.…”

“…[ 32 ] Two (18.2%) studies employed definite (microbiologic and pathologic) reference criteria for diagnosing TPE. [ 24 30 ] Most studies (7, 63.6%) assayed lysozyme through a turbidimetric method [ Table 1 ]. [ 22 26 27 28 29 31 32 ]…”

“…Seven studies reported information regarding diagnostic test accuracy of LP/LS on 224 TPE patients and 630 patients having pleural effusions due to other disorders [ Table S1 of online supplement ]. [ 23 24 27 28 30 31 32 ] Four of these studies also provided data for diagnostic test accuracy of LP on 88 TPE patients and 267 patients having pleural effusions due to other disorders [ Table S1 of online supplement ]. [ 23 27 28 30 ]Only one (14.3%) study was published from a high TB burden country.…”

“…[ 27 ] Five (71.4%) studies included patients only with an exudative pleural effusion. [ 23 24 30 31 32 ] Only one (14.3%) study performed assays in a blinded fashion. [ 32 ] Four (57.1%) studies employed a composite reference standard to diagnose TPE.…”

Pleural fluid lysozyme as a diagnostic biomarker of pleural tuberculosis

“…Our literature search yielded 89 citations [ Figure 1 ], of which we ultimately included 11 studies reporting information on diagnostic accuracy ( N = 7) or comparing LP data between tuberculous and other effusions ( N = 10). [ 22 23 24 25 26 27 28 29 31 32 ] The major attributes of these 11 publications are summarized in Table 1 . The maximum number of studies (5, 45.5%) were conducted in Spain.…”

“…The maximum number of studies (5, 45.5%) were conducted in Spain. [ 22 24 30 31 32 ] Only two studies (18.2%), both from India, were conducted in a country with a high burden of TB. [ 27 29 ] One Indian study reported data exclusively from pediatric subjects.…”

“…[ 32 ] Two (18.2%) studies employed definite (microbiologic and pathologic) reference criteria for diagnosing TPE. [ 24 30 ] Most studies (7, 63.6%) assayed lysozyme through a turbidimetric method [ Table 1 ]. [ 22 26 27 28 29 31 32 ]…”

“…Seven studies reported information regarding diagnostic test accuracy of LP/LS on 224 TPE patients and 630 patients having pleural effusions due to other disorders [ Table S1 of online supplement ]. [ 23 24 27 28 30 31 32 ] Four of these studies also provided data for diagnostic test accuracy of LP on 88 TPE patients and 267 patients having pleural effusions due to other disorders [ Table S1 of online supplement ]. [ 23 27 28 30 ]Only one (14.3%) study was published from a high TB burden country.…”

“…[ 27 ] Five (71.4%) studies included patients only with an exudative pleural effusion. [ 23 24 30 31 32 ] Only one (14.3%) study performed assays in a blinded fashion. [ 32 ] Four (57.1%) studies employed a composite reference standard to diagnose TPE.…”

Pleural fluid lysozyme as a diagnostic biomarker of pleural tuberculosis

“…Our literature search yielded 89 citations [ Figure 1 ], of which we ultimately included 11 studies reporting information on diagnostic accuracy ( N = 7) or comparing LP data between tuberculous and other effusions ( N = 10). [ 22 23 24 25 26 27 28 29 31 32 ] The major attributes of these 11 publications are summarized in Table 1 . The maximum number of studies (5, 45.5%) were conducted in Spain.…”

“…The maximum number of studies (5, 45.5%) were conducted in Spain. [ 22 24 30 31 32 ] Only two studies (18.2%), both from India, were conducted in a country with a high burden of TB. [ 27 29 ] One Indian study reported data exclusively from pediatric subjects.…”

“…[ 32 ] Two (18.2%) studies employed definite (microbiologic and pathologic) reference criteria for diagnosing TPE. [ 24 30 ] Most studies (7, 63.6%) assayed lysozyme through a turbidimetric method [ Table 1 ]. [ 22 26 27 28 29 31 32 ]…”

“…Seven studies reported information regarding diagnostic test accuracy of LP/LS on 224 TPE patients and 630 patients having pleural effusions due to other disorders [ Table S1 of online supplement ]. [ 23 24 27 28 30 31 32 ] Four of these studies also provided data for diagnostic test accuracy of LP on 88 TPE patients and 267 patients having pleural effusions due to other disorders [ Table S1 of online supplement ]. [ 23 27 28 30 ]Only one (14.3%) study was published from a high TB burden country.…”

“…[ 27 ] Five (71.4%) studies included patients only with an exudative pleural effusion. [ 23 24 30 31 32 ] Only one (14.3%) study performed assays in a blinded fashion. [ 32 ] Four (57.1%) studies employed a composite reference standard to diagnose TPE.…”

Pleural fluid lysozyme as a diagnostic biomarker of pleural tuberculosis

“…Our literature search yielded 89 citations [ Figure 1 ], of which we ultimately included 11 studies reporting information on diagnostic accuracy ( N = 7) or comparing LP data between tuberculous and other effusions ( N = 10). [ 22 23 24 25 26 27 28 29 31 32 ] The major attributes of these 11 publications are summarized in Table 1 . The maximum number of studies (5, 45.5%) were conducted in Spain.…”

“…The maximum number of studies (5, 45.5%) were conducted in Spain. [ 22 24 30 31 32 ] Only two studies (18.2%), both from India, were conducted in a country with a high burden of TB. [ 27 29 ] One Indian study reported data exclusively from pediatric subjects.…”

“…[ 32 ] Two (18.2%) studies employed definite (microbiologic and pathologic) reference criteria for diagnosing TPE. [ 24 30 ] Most studies (7, 63.6%) assayed lysozyme through a turbidimetric method [ Table 1 ]. [ 22 26 27 28 29 31 32 ]…”

“…Seven studies reported information regarding diagnostic test accuracy of LP/LS on 224 TPE patients and 630 patients having pleural effusions due to other disorders [ Table S1 of online supplement ]. [ 23 24 27 28 30 31 32 ] Four of these studies also provided data for diagnostic test accuracy of LP on 88 TPE patients and 267 patients having pleural effusions due to other disorders [ Table S1 of online supplement ]. [ 23 27 28 30 ]Only one (14.3%) study was published from a high TB burden country.…”

“…[ 27 ] Five (71.4%) studies included patients only with an exudative pleural effusion. [ 23 24 30 31 32 ] Only one (14.3%) study performed assays in a blinded fashion. [ 32 ] Four (57.1%) studies employed a composite reference standard to diagnose TPE.…”