1992
DOI: 10.1128/aem.58.12.4064-4067.1992
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Development of a Method for Detection of Lactic Acid Bacteria Producing Exclusively the l -(+)- Isomer of Lactic Acid

Abstract: A method was developed for the detection and isolation, within a population of lactic acid bacteria, of strains producing exclusively the L-(+)isomer of lactic acid; the visual detection of colonies of these particular strains can be carried out directly on agar plates (50 to 70 colonies per plate). The method is based on an enzymatic stereospecific reaction involving D-(-)-lactate dehydrogenase and linked to a staining reaction; the diffusion area of the D-(-)isomer stains red around the D-(-)and the DL-lacti… Show more

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Cited by 12 publications
(1 citation statement)
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“…L-lactic acid has a greater inhibitory activity than its D-isomer 11 . From a nutritional aspect, the formation of D(-)-lactate by LAB in fermented foods and beverages is undesirable, because D(-)-lactate is not readily metabolised by humans compared to L(+)-lactate 66,95 . LAB have been exploited for centuries in brewing and wine fermentations.…”
Section: Lactic Acid Bacteria (Lab)mentioning
confidence: 99%
“…L-lactic acid has a greater inhibitory activity than its D-isomer 11 . From a nutritional aspect, the formation of D(-)-lactate by LAB in fermented foods and beverages is undesirable, because D(-)-lactate is not readily metabolised by humans compared to L(+)-lactate 66,95 . LAB have been exploited for centuries in brewing and wine fermentations.…”
Section: Lactic Acid Bacteria (Lab)mentioning
confidence: 99%