Development of a McAb-based immunoassay for parathion and influence of the competitor structure

Wang, Chunmei; Liu, Yihua; Guo, Yirong; Liang, Chizhou; Li, Xianbin; Zhu, Guonian

doi:10.1016/j.foodchem.2008.11.091

Cited by 41 publications

(31 citation statements)

References 27 publications

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“…[15] The synthesis strategy and Mab production procedures were described in our studies on parathion ELISA determination. [16,17] The hapten synthesis method is simply described as follows: A solution of 3,5,6-trichloropyridin-2-ol (21.6 mmol) in 15 mL of acetonitrile was added dropwise to a stirred mixture of ethyl dichlorothiophosphate (27.8 mmol), 20 g of finely ground K 2 CO 3 and 20 mL of acetonitrile. After stirring for 1 h at room temperature, the mixture was filtered through celite, and the solvent was removed under reduced pressure.…”

Section: Preparation Of Hapten-protein Conjugatementioning

confidence: 99%

A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples

Liu

Chen

Guo

et al. 2011

Journal of Environmental Science and Health, Part B

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A monoclonal antibody-based competitive antibody-coated enzyme-linked immunosorbent assay (ELISA) was developed and optimized for determining chlorpyrifos residue in agricultural products. The IC 50 and IC 10 of this ELISA were 3.3 ng/mL and 0.1 ng/mL respectively. The average recoveries in six agricultural products were between 79.5% and 118.0%, with the intra-assay coefficient of variation being less than 8 %. The limit of detection for all tested products was 30 ng/g. To the best of our knowledge, this assay has the best specificity among all the published research on ELISAs for chlorpyrifos.

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Section: Preparation Of Hapten-protein Conjugatementioning

confidence: 99%

A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples

Liu

Chen

Guo

et al. 2011

Journal of Environmental Science and Health, Part B

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“…Splenocytes of selected mice were harvested aseptically. Cell fusion and hybridoma selection procedures were performed essentially as described previously (Li, Zhang, Zhang, Zhang, & Chen, 2009b;Liu et al, 2009;Wang, Zhang, Chen, Liu, & Li, 2011;Wang et al, 2009;Zeng et al, 2007;Zhang et al, 2007). Briefly, the splenocytes were added to myeloma cells to give a ratio of five splenocytes per myeloma cell and the mixture was centrifuged.…”

Section: Production Of Monoclonal Antibodymentioning

confidence: 99%

An enzyme-linked chemiluminescent immunoassay developed for detection of Butocarboxim from agricultural products based on monoclonal antibody

et al. 2015

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“…Total RNA was extracted from 10 7 hybridoma cells (reported in our previous study [17] ) that secreted mAb (5H2) against parathion with RNAiso Reagent kit (Takara Biotechnology (Dalian) Inc., China), according to the manufacturer's instructions. Then the first-strand DNA was synthesized using reverse transcriptase and Oligo(dt) 18 Primer (RevertAid TM first Strand cDNA Synthesis Kit, Fermentas Inc., Germany).…”

Section: Total Rna Extraction and Cdna Synthesismentioning

confidence: 99%

“…In our previous studies, high specific polyclonal antibody (pAb) [16] and monoclonal antibody (mAb) [17] against parathion were obtained. In the present study, we selected the hybridoma cells that secreted anti-parathion mAb (5H2) as the object, the DNA sequence of the antibody was analyzed.…”

Section: Introductionmentioning

confidence: 99%

Homology modeling of anti-parathion antibody and its interaction with organophosphorous pesticides and analogues

Liu

Guo

Wang

et al. 2010

Journal of Environmental Science and Health, Part B

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The mechanism of specific recognition in pesticide immunochemistry was investigated by computer-based strategy, and a rapid method for the identification of antibody specificity was developed. Based on the previously produced anti-parathion monoclonal antibody (mAb), the DNA sequence was analyzed by polymerase chain reaction (PCR). From the translated amino acid sequences, a three-dimensional structure of the antibody was constructed by homology modeling method, and then it was coordinated by 1 ns molecular dynamics under the explicit solvent condition. The stereochemical property and folding quality were further assessed by Procheck and Profile-3D. The self-compatibility score for the antibody model was 98.7, which was greater than the low score 46.2 and close to the top score 102.6. In addition, parathion and several structural analogues were docked to the constructed antibody structure. The docking results showed that the interaction energy (-40.54 kcal/mol) of antibody-parathion complex was the lowest among all the tested pesticides, which accounted for the high specificity of the antibody to parathion and perfectly matched with the experimental data. Moreover, three residues, Phe165, Asp107 and Thr100 were recognized as the most important residues for antibody reacting with parathion. The interaction energy negatively correlated with the antibody specificity.

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Development of a McAb-based immunoassay for parathion and influence of the competitor structure

Cited by 41 publications

References 27 publications

A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples

A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples

An enzyme-linked chemiluminescent immunoassay developed for detection of Butocarboxim from agricultural products based on monoclonal antibody

Homology modeling of anti-parathion antibody and its interaction with organophosphorous pesticides and analogues

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