Development of a liquid chromatography–tandem mass spectrometry with ultrasound-assisted extraction and auto solid-phase clean-up method for the determination of Fusarium toxins in animal derived foods

Chen, Dongmei; Cao, Xiaoqin; Tao, Yanfei; Wu, Qinghua; Pan, Yuanhu; Peng, Dapeng; Liu, Zhenli; Huang, Lingli; Wang, Yulian; Wang, Xu; Zhang, Yuan

doi:10.1016/j.chroma.2013.08.062

Cited by 30 publications

(18 citation statements)

References 44 publications

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“…The method can be considered as an alternative to LC-MS/MS and could be adopted by nonprofessional analytical labs. [11]. The CCα and CCβ of the analytes in different samples varied 0.16 -1.37 μg/kg and 0.33 -2.34 μg/kg, respectively.…”

Section: Mass Spectrometry Analytical Methodsmentioning

confidence: 92%

“…Analytical methods for rapid, sensitive, and accurate determination of DON and its derivatives in foods, feeds, human and animals are highly demanded for toxicological analysis and exposure risk assessment. For the confirmative determination of DON in food, a mass-spectrometric (MS) method would be the most suitable due to its unambiguous identification and accurate quantification possibilities [11]. Currently, many specific and sensitive HPLC-MS/MS methods for the simultaneous determination of DON and other mycotoxins in food and feed are well developed [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Deoxynivalenol, a Trichothecene Mycotoxin: Review of Its Masked Form, Contamination in Cereal-Based Feed, and Mass Spectrometry Analytical Methods

Zhang¹,

Huang²,

Wan³

et al. 2015

Mil. Med. Sci. Lett.

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Deoxynivalenol (DON) is a trichothecene mycotoxin regularly occurring in cereals. It is toxic to humans and inhibits DNA, RNA and protein synthesis. Moreover, its conjugated products, called masked mycotoxins, including DON-3-glucoside (DON-3G), is another "emerging" food safety issue in recent years. In this review, we first discussed the nature of contamination of masked DON in cereal-based food and feed; moreover, the new reported masked DON is summarized and we also focused on its toxicity and digestion in human and animal bodies. In addition, the contamination of DON in cereal-based feed in different countries was summarized and some regular patterns of DON occurrence are suggested. Finally, the LC-MS methods for the determination of DON and masked DON in food, feed and animal fluids were compared. This review will provide further information for DON and masked DON contamination and shed some light on the mycotoxin control strategies.

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Section: Mass Spectrometry Analytical Methodsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Deoxynivalenol, a Trichothecene Mycotoxin: Review of Its Masked Form, Contamination in Cereal-Based Feed, and Mass Spectrometry Analytical Methods

Zhang¹,

Huang²,

Wan³

et al. 2015

Mil. Med. Sci. Lett.

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“…Chen et al developed a method for analysis of aflatoxins and ochratoxin A in animal products [21]. Subsequently, the same author developed another method for determination of Fusarium toxins in animal products [22]. However, to the best of our knowledge, there have not been any reports concerning simultaneous determination of multiclass mycotoxins in various animal products.…”

Section: Introductionmentioning

confidence: 96%

Multi-mycotoxin analysis of animal feed and animal-derived food using LC–MS/MS system with timed and highly selective reaction monitoring

et al. 2015

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Mycotoxins have the potential to enter the human food chain through carry-over of contaminants from feed into animal-derived products. The objective of the study was to develop a reliable and sensitive method for the analysis of 30 mycotoxins in animal feed and animal-derived food (meat, edible animal tissues, and milk) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In the study, three extraction procedures, as well as various cleanup procedures, were evaluated to select the most suitable sample preparation procedure for different sample matrices. In addition, timed and highly selective reaction monitoring on LC-MS/MS was used to filter out isobaric matrix interferences. The performance characteristics (linearity, sensitivity, recovery, precision, and specificity) of the method were determined according to Commission Decision 2002/657/EC and 401/2006/EC. The established method was successfully applied to screening of mycotoxins in animal feed and animal-derived food. The results indicated that mycotoxin contamination in feed directly influenced the presence of mycotoxin in animal-derived food. Graphical abstract Multi-mycotoxin analysis of animal feed and animal-derived food using LC-MS/MS.

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“…The important issue is the simultaneous determination of DON and its modified forms. Various methods for their analysis in cereals and feedstuffs have been reported, such as liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) [ 13 , 14 , 15 , 16 ], fluorescence detection (FLD) [ 17 ], photodiode-array detection (PDA) [ 18 , 19 , 20 ], and ultra-high-performance supercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS) [ 21 ]. Moreover, in recent years the introduction of high-resolution mass spectrometers (HRMS) has allowed screening of non-target compounds, novel compound identification and retrospective data analysis [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…Different sample preparation techniques and clean-up approaches have been used in feedstuffs: solid-liquid-extraction (SLE) without clean-up [ 16 , 23 ], the quick, easy, cheap, effective, rugged and safe (QuEChERS) technique [ 24 ], solid-phase-extraction (SPE) [ 14 ] and immunoaffinity columns (IAC) [ 17 , 25 , 26 ]. While SLE is frequently applied for multi-mycotoxin analysis in feedstuffs, the inclusion of clean-up strategies in the procedure could increase the sensitivity of the method, as well as decrease high matrix effect for the difficult matrix.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography–Tandem Mass Spectrometry Method

Panasiuk

Jedziniak

Pietruszka

et al. 2020

Toxins

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A liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3Ac-DON), 15-acetyldeoxynivalenol (15Ac-DON), DON-3-glucoside (DON-3Glc) nivalenol and fusarenone-X in feedstuffs. Different techniques of sample preparation were tested: solid-liquid-extraction, QuEChERS, solid phase extraction with OASIS HLB columns or immunoaffinity columns and a Mycosep 225 Trich column. None of the six immunoaffinity columns tested showed cross-reactivity to all of the mycotoxins. Surprisingly, the results show that if the immunoaffinity columns bound 3Ac-DON, then they did not bind 15Ac-DON. The most efficient sample preparation was achieved with a Mycosep 225 Trich column clean-up. The chromatography was optimised to obtain full separation of all analytes (including 3Ac-DON and 15Ac-DON isomeric form). The validation results show the relative standard deviations for repeatability and reproducibility varied from 4% to 24%. The apparent recovery ranged between 92% and 97%, and the limit of quantification described a 1.30 to 50 µg/kg range. The method trueness was satisfactory, as assessed by a proficiency test and analysis of reference material. A total of 99 feed samples were analysed by the developed method, revealing the presence of DON and DON-3Glc in 85% and 86% of examined animal feeds, respectively at concentrations between 1.70 and 1709 µg/kg. The ratios DON-3Glc to DON in the surveyed feedstuffs were from a low of 3% to high of 59%.

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Development of a liquid chromatography–tandem mass spectrometry with ultrasound-assisted extraction and auto solid-phase clean-up method for the determination of Fusarium toxins in animal derived foods

Cited by 30 publications

References 44 publications

Deoxynivalenol, a Trichothecene Mycotoxin: Review of Its Masked Form, Contamination in Cereal-Based Feed, and Mass Spectrometry Analytical Methods

Deoxynivalenol, a Trichothecene Mycotoxin: Review of Its Masked Form, Contamination in Cereal-Based Feed, and Mass Spectrometry Analytical Methods

Multi-mycotoxin analysis of animal feed and animal-derived food using LC–MS/MS system with timed and highly selective reaction monitoring

Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography–Tandem Mass Spectrometry Method

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