volume 18, issue 5, P347-352 1996
DOI: 10.1016/0141-0229(95)00110-7
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Alicia E. Gardiol, Ruben J. Hernandez, Bengt Reinhammar, Bruce R. Harte

Abstract: A gas-phase oxygen biosensor based on blue copper-containing oxidases was developed. Blue-oxidase enzymes, including laccase and ascorbate oxidase, have a blue chromophore prosthetic group, type 1 Cu+2, which can be reduced and decolorized with reducing substrates. When the enzyme is reoxidized with molecular oxygen, there is a concomitant return of the blue color. The oxygen biosensor consisted of the Rhus vernicifera laccase and ascorbate as substrate enclosed in pouches of low-density polyethylene under nit…

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