Development of a fast and simple liquid chromatography–tandem mass spectrometry method for the quantitation of argatroban in patient plasma samples

Rhea, Jeanne M.; Snyder, Marion L.; Winkler, Anne; Abou‐Diwan, Charbel; Fantz, Corinne R.; Ritchie, James C.; Szlam, Fania; Tanaka, Kenichi A.; Molinaro, Ross J.

doi:10.1016/j.jchromb.2012.02.041

Cited by 10 publications

(7 citation statements)

References 18 publications

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“…Liquid chromatography/mass spectrometry may also be used to measure each drug but requires equipment that may not be readily available in most laboratories. 13,18 are currently available and widely used, and laboratories are asked to assist with patient management on a routine basis. Theoretically, a number of tests may be used to measure the DTI content of plasma.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the aPTT With Alternative Tests for Monitoring Direct Thrombin Inhibitors in Patient Samples

Lind

Boyle

Fisher

et al. 2014

American Journal of Clinical Pathology

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Section: Discussionmentioning

confidence: 99%

Comparison of the aPTT With Alternative Tests for Monitoring Direct Thrombin Inhibitors in Patient Samples

Lind

Boyle

Fisher

et al. 2014

American Journal of Clinical Pathology

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“…This method has a detection threshold value of < 0, 1 U ml −1 (Gerinnungspraxis Mannheim, Germany, Prof. Dr. C. E. Dempfle). Direct measurement of argatroban concentration was performed by high performance liquid chromatography and double mass spectrometry detection (Analytical Clinical Concepts GmbH; Leidersbach) [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Argatroban for anticoagulation of a blood salvage system - an ex-vivo study

et al. 2015

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BackgroundBlood salvage systems help to minimize intraoperative transfusion of allogenic blood. So far no data is available on the use of argatroban for anticoagulation of such systems.We conducted an ex-vivo trial to evaluate the effectiveness of three different argatroban doses as compared to heparin and to assess potential residual anticoagulant in the red cell concentrates.MethodsWith ethical approval and individual informed consent, blood of 23 patients with contraindications for use of blood salvage systems during surgery was processed by the Continuous-Auto-Transfusion-System (C.A.T.S. ® Cell Saver System, Fresenius Kabi, Bad Homburg, Germany) using 5,50 or 250 mg of argatroban or 25.000 U of heparin in 1000 ml saline for anticoagulation of the system. Emergency and high-quality washing modes were applied in random order. Patency of the system and residual amount of anticoagulants in the re-transfusion bag were measured. The collected blood was not re-infused, but only used for analysis of hematocrit, heparin and argatroban concentrations.ResultsPatency of the system was provided by all anticoagulants except for 3/8 cases with 5 mg of argatroban. Residual anticoagulant was found in 2/10 (20 %) heparin samples in two different patients (1 emergency and 1 high-quality washing) and in all argatroban samples. High quality washing eliminated 89–95 % and emergency washing 60–90 % of the initial argatroban concentration. Residual argatroban concentrations ranged from 55 ng ml−1 to 6810 ng ml−1, with initial argatroban concentrations of 5 and 250 mg, respectively.ConclusionThe C.A.T.S. does not reliably remove heparin and should therefore not be used in HIT patients. Anticoagulation with 50 and 250 mg argatroban, maintains the systems patency and is significantly removed during washing. In this ex-vivo study a concentration of 50 μg ml−1 argatroban provided the best ratio of system patency and residual argatroban concentration. Additional dose-finding studies with different blood salvage systems are needed to evaluate the optimal argatroban concentration.

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“…Each sample (10 µL) was analyzed in a UPLC-ESI-MS/MS system (LCQ Fleet, Thermo Finnigan, San Jose, CA, USA). This equipment used a C18 Hypersil gold column (50 × 2.1 × 1.9 mm) [28]. Operating conditions were as follows: oven temperature of 38 • C, flow rate of 350 L/min, where gradient mobile phase A was water in 0.1% formic acid and mobile phase B was methanol in 0.1% formic acid.…”

Section: Analysis Of Metabolites By Uplc-esi-ms/msmentioning

confidence: 99%

Evaluation of Metabolomic Profile and Growth of Moringa oleifera L. Cultivated with Vermicompost under Different Soil Types

et al. 2021

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Moringa oleifera is a highly versatile plant with potential use in the agro-food and biochemical industry. The goals of this study were to evaluate the effect of chemical fertilization and vermicompost on plant growth, and to analyze the metabolomic profile of M. oleifera crops cultivated over agricultural and native soils. The extracts were obtained from 90-day-old leaves via extraction with a hydroalcoholic mixture. Multivariate data analyses, such as principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA), were used to differentiate the distribution of leaf metabolites according to the soils or types of fertilizers used for the cultivation of Moringa oleifera. The results indicated that there was no significant effect on parameters such as plant height, root length and dry weight of leaves (p < 0.05). UPLC-ESI-MS/MS analysis of leaf extracts revealed a wide range of flavonoids, alkaloids and organic acids. The results of PCA and PLS-DA confirmed that the type of fertilizer had an effect on the metabolomic profile of M. oleifera leaves. The application of vermicompost induced changes in the metabolomic profile, but not in the morphometric variables of Moringa oleifera. These results are important for metabolite production via organic cultures and over different soil types in the industrialization of Moringa.

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Development of a fast and simple liquid chromatography–tandem mass spectrometry method for the quantitation of argatroban in patient plasma samples

Cited by 10 publications

References 18 publications

Comparison of the aPTT With Alternative Tests for Monitoring Direct Thrombin Inhibitors in Patient Samples

Comparison of the aPTT With Alternative Tests for Monitoring Direct Thrombin Inhibitors in Patient Samples

Argatroban for anticoagulation of a blood salvage system - an ex-vivo study

Evaluation of Metabolomic Profile and Growth of Moringa oleifera L. Cultivated with Vermicompost under Different Soil Types

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