Development of a DNA Microarray for Toxicology Based on Hepatotoxin-Regulated Sequences

Abstract

Abbreviations: ip, intraperitoneal; iv, intravenous; ND, not done. a All animals in this treatment group died before day 3 and were not used. Where no reference is provided, dose levels were selected from unpublished results.

Molecular biomarkers in grey seals (Halichoerus grypus) to evaluate pollutant exposure, health and immune status

“…There was no difference in gene expression in grey seal pups from North and Baltic Sea while there were clear species-specific differences between grey and harbour seal pups. Differences in gene expression between pups and adult grey seals and inter-individual variation in adults show that it is critical to detect the biological variability within individuals and species to recognize changes that may be induced by pollutants (Boorman et al, 2005) and to associate gene patterns with specific toxicants (Bulera et al, 2001;Waring et al, 2003) and health status. Molecular biomarkers may become early and sensitive tools to assess health, toxicant-induced and immune-relevant physiological mechanisms in marine mammals.…”

Molecular biomarkers in grey seals (Halichoerus grypus) to evaluate pollutant exposure, health and immune status

“…There was no difference in gene expression in grey seal pups from North and Baltic Sea while there were clear species-specific differences between grey and harbour seal pups. Differences in gene expression between pups and adult grey seals and inter-individual variation in adults show that it is critical to detect the biological variability within individuals and species to recognize changes that may be induced by pollutants (Boorman et al, 2005) and to associate gene patterns with specific toxicants (Bulera et al, 2001;Waring et al, 2003) and health status. Molecular biomarkers may become early and sensitive tools to assess health, toxicant-induced and immune-relevant physiological mechanisms in marine mammals.…”

Identification of classifier genes for hepatotoxicity prediction in non steroidal anti inflammatory drugs

“…The ultimate purpose of toxicogenomics is developing a toxicity prediction system using suitable classifier probes through microarray techniques as a replacement of traditional toxicity tests 2,10, [16][17][18][21][22][23][24][25][26][27] . Also, it is important to accumulate data on classifier probes of sufficient numbers of drugs in order to predict, but there are studies using only a few drugs 8,28,29 .…”

Prediction and Classification of Drug Toxicity Using Probabilistic Modeling of Temporal Metabolic Data: The Consortium on Metabonomic Toxicology Screening Approach

“…Urinary metabonomics gives a systems-level view of organism response without the need to identify all potentially affected tissues a priori. Toxicity prediction studies using in vivo gene expression profiling [37][38][39] have to date also suffered from small numbers of treatments employed in the validation of predictions 40 as well as the lack of concurrent data from conventional techniques (such as histopathology) to define the true class labels. 41 In vitro approaches are in development 42 but, as yet, are unable to reproduce the complex physiological interactions that are often involved in systemic toxicity.…”

Molecular biomarkers in grey seals (Halichoerus grypus) to evaluate pollutant exposure, health and immune status

“…There was no difference in gene expression in grey seal pups from North and Baltic Sea while there were clear species-specific differences between grey and harbour seal pups. Differences in gene expression between pups and adult grey seals and inter-individual variation in adults show that it is critical to detect the biological variability within individuals and species to recognize changes that may be induced by pollutants (Boorman et al, 2005) and to associate gene patterns with specific toxicants (Bulera et al, 2001;Waring et al, 2003) and health status. Molecular biomarkers may become early and sensitive tools to assess health, toxicant-induced and immune-relevant physiological mechanisms in marine mammals.…”

Identification of classifier genes for hepatotoxicity prediction in non steroidal anti inflammatory drugs

“…The ultimate purpose of toxicogenomics is developing a toxicity prediction system using suitable classifier probes through microarray techniques as a replacement of traditional toxicity tests 2,10, [16][17][18][21][22][23][24][25][26][27] . Also, it is important to accumulate data on classifier probes of sufficient numbers of drugs in order to predict, but there are studies using only a few drugs 8,28,29 .…”

Prediction and Classification of Drug Toxicity Using Probabilistic Modeling of Temporal Metabolic Data: The Consortium on Metabonomic Toxicology Screening Approach

“…Urinary metabonomics gives a systems-level view of organism response without the need to identify all potentially affected tissues a priori. Toxicity prediction studies using in vivo gene expression profiling [37][38][39] have to date also suffered from small numbers of treatments employed in the validation of predictions 40 as well as the lack of concurrent data from conventional techniques (such as histopathology) to define the true class labels. 41 In vitro approaches are in development 42 but, as yet, are unable to reproduce the complex physiological interactions that are often involved in systemic toxicity.…”

Molecular biomarkers in grey seals (Halichoerus grypus) to evaluate pollutant exposure, health and immune status

“…There was no difference in gene expression in grey seal pups from North and Baltic Sea while there were clear species-specific differences between grey and harbour seal pups. Differences in gene expression between pups and adult grey seals and inter-individual variation in adults show that it is critical to detect the biological variability within individuals and species to recognize changes that may be induced by pollutants (Boorman et al, 2005) and to associate gene patterns with specific toxicants (Bulera et al, 2001;Waring et al, 2003) and health status. Molecular biomarkers may become early and sensitive tools to assess health, toxicant-induced and immune-relevant physiological mechanisms in marine mammals.…”

Identification of classifier genes for hepatotoxicity prediction in non steroidal anti inflammatory drugs

“…The ultimate purpose of toxicogenomics is developing a toxicity prediction system using suitable classifier probes through microarray techniques as a replacement of traditional toxicity tests 2,10, [16][17][18][21][22][23][24][25][26][27] . Also, it is important to accumulate data on classifier probes of sufficient numbers of drugs in order to predict, but there are studies using only a few drugs 8,28,29 .…”

Prediction and Classification of Drug Toxicity Using Probabilistic Modeling of Temporal Metabolic Data: The Consortium on Metabonomic Toxicology Screening Approach

“…Urinary metabonomics gives a systems-level view of organism response without the need to identify all potentially affected tissues a priori. Toxicity prediction studies using in vivo gene expression profiling [37][38][39] have to date also suffered from small numbers of treatments employed in the validation of predictions 40 as well as the lack of concurrent data from conventional techniques (such as histopathology) to define the true class labels. 41 In vitro approaches are in development 42 but, as yet, are unable to reproduce the complex physiological interactions that are often involved in systemic toxicity.…”