Development of a class-specific polyclonal antibody-based indirect competitive ELISA for detecting fluoroquinolone residues in milk

Fan, Guoying; Yang, Ruosong; Jiang, Jinqing; Chang, Xin; Chen, Junjie; You, Qi; Wu, Shixiu; Yang, Xuefeng

doi:10.1631/jzus.b1200001

Cited by 26 publications

(17 citation statements)

References 18 publications

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“…Many ELISA methods were applied for the detection of numerous antibiotic residues such as for fluoroquinolones (FQs) and chloramphenicol (CAP). The competitive ELISA methods for detecting the FQs were mainly based on drug-bovine serum albumin (BSA) conjugate and specific polyclonal antibody (Fan, Yang et al, 2012;Huang, 2014). While for the CAP and tetracycline (TC), mainly the principle of specific monoclonal antibody was followed (Gao, Zhao, Zhang, Wang, & Wang, 2013;Le, Yu, Zhao, & Wei, 2012;Liu et al, 2014).…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

119

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Food contamination with antibiotic residues has become a worldwide problem due to the heavy and improper use of antibiotics. The development of fast, high-throughput, easy, and economical screening methods for the detection of antibiotic residues is an important requirement as an alternative to the traditional assays. Immunoassays are widespread promising methods that can achieve the conditions of an analytical method in the evaluation of food and environmental protection. This review offers an overview of the preparation of antibodies and the currently established immuneassays for the antibiotic residues detection, including enzymelinked immunosorbent assay, fluorescence immunoassay, radioimmunoassay, colloidal gold immunoassay, and chemiluminescence immunoassay. Furthermore, it will elaborate on the future perspective on the performance and improvement of these assays for the determination of antibiotic residues in food samples. These assays include a simple sample preparation and high-sensitive detection that offer a significant assurance for the screening of antibiotic residues.

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Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

119

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“…The limit of detection (LOD) was defined A c c e p t e d M a n u s c r i p t 8 as the lowest analyte concentration that was determined by the mean background levels plus three times standard deviation (B 0 +3s) . [25][26] …”

Section: The Standard Curve and Sensitivity Of The Sandwich Elisamentioning

confidence: 99%

Detection of Penicillinase in Milk by Sandwich ELISA Based Polyclonal and Monoclonal Antibody

Zhao¹,

Li²

2015

Journal of Immunoassay and Immunochemistry

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A sandwich ELISA has been developed using polyclonal and monoclonal antibody for the determination of penicillinase in milk. For this purpose, specific polyclonal and monoclonal antibodies against penicillinase were generated and characterized. Using penicillinase standards prepared from 1-128 ng/mL, the method indicated that the detection limit of the sandwich ELISA, as measured in an ELISA plate reader, was as low as 0.86 ng/mL of penicillinase. For determine the accuracy, raw milk containing 2, 8, 32, and 64 ng/mL of penicillinase were tested by sandwich ELISA. Recoveries were from 93-97.5%, and the coefficient of variation [CV (%)] were from 5.55-8.38%. For interassay reproducibility, recoveries were from 89.5-95.1%, the coefficient of variation [CV (%)] were from 5.26-9.58%. This sandwich ELISA provides a useful screening method for quantitative detection of penicillinase in milk.

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“…Enzyme-linked immunosorbent assay (ELISA), which is based on specific antigen-antibody interactions, is the most suitable method for rapid screening of ENR residue in the veterinary field [29,30]. Monoclonal or polyclonal antibodies have been developed for use in immunochemical detection assays [9,20]. Many organic solvents or immunoaffinity columns are required to separate FQs from the matrix to enable their analysis.…”

Section: Introductionmentioning

confidence: 99%

Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin

Kim

Park

et al. 2015

J Vet Sci

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Monoclonal anti-enrofloxacin antibody was prepared for a direct competitive enzyme-linked immunosorbent assay (ELISA) and purification system using monoclonal antibody (mAb) coupled magnetic nanoparticles (MNPs). The IC50 values of the developed mAb for enrofloxacin (ENR), ciprofloxacin, difloxacin, sarafloxacin, pefloxacin, and norfloxacin were 5.0, 8.3, 9.7, 21.7, 36.0, and 63.7 ng/mL, respectively. The lowest detectable level of ENR was 0.7 ng/mL in the prepared ELISA system. To validate the developed ELISA in the food matrix, known amounts of ENR were spiked in meat and egg samples at 10, 20 and 30 ng/mL. Recoveries for ENR ranged from 72.9 to 113.16% with a coefficient of variation (CV) of 2.42 to 10.11%. The applicability of the mAb-MNP system was verified by testing the recoveries for ENR residue in three different matrices. Recoveries for ENR ranged from 75.16 to 86.36%, while the CV ranged from 5.08 to 11.53%. Overall, ENR-specific monoclonal antibody was prepared and developed for use in competitive to ELISAs for the detection of ENR in animal meat samples. Furthermore, we suggest that a purification system for ENR using mAb-coupled MNPs could be useful for determination of ENR residue in food.

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Development of a class-specific polyclonal antibody-based indirect competitive ELISA for detecting fluoroquinolone residues in milk

Cited by 26 publications

References 18 publications

Current advances in immunoassays for the detection of antibiotics residues: a review

Current advances in immunoassays for the detection of antibiotics residues: a review

Detection of Penicillinase in Milk by Sandwich ELISA Based Polyclonal and Monoclonal Antibody

Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin

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