Development of a chromatographic fingerprint for the chloroform extracts of Ganoderma lucidum by HPLC and LC–MS

Traditional Chinese Herbal Medicine – East Meets West in Validation and Therapeutic Application

“…Toxicities were rarely reported, and the safe usage has been convinced by scientific www.intechopen.com evidence and history (Mizuno et al, 1995). Several Lingzhi products have already been well characterized by fingerprinting and genotyping techniques for authentication and quality control (Chen et al, 2008a(Chen et al, , 2008b. The above conditions explicitly supported the clinical trials at phase I and II.…”

“…Additionally, there are some pattern recognition methods (some sort of statistical methods), which enable the visualization and further exploratory data analysis on information that is included in the chromatographic profile, are available for addressing the difficult differentiation of some closely related species (Lu et al, 2005;Tistaert et al, 2011;Zhao et al, 2009). The quality of herbs may vary due to the cultivation conditions, breeds and places of origin, for examples, liquid chromatographic coupled with multistage mass spectrometry (HPLC-MS) technique has revealed there to be non-stable and inconsistence of chemical constituents amongst different batches of Lingzhi samples (Chen et al, 2008a). In this regard, the research team (Chen et al, 2008b) has demonstrated the feasibility of employing multiple statistical analyses of HPLC fingerprints of Lingzhi to discriminate samples in accordance of origin of cultivation (Chen et al, 2008b).…”

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

“…A total of 20 furocoumarins were identified or tentatively characterized. A fingerprinting method for the quality control of Ganoderma lucidum using HPLC and LC-MS was published by Chen et al [220]. Twenty-nine batches obtained from three different origins in China were used to establish the fingerprint.…”

Inhibitory Effects of the Methanol Extract of Ganoderma lucidum on Mosquito Allergy–Induced Itch-Associated Responses in Mice

“…The flow rate was 0.2 ml/min. Annotation of an unknown compound was preferably assigned to a compound that had been previously reported on G. lucidum by comparing the high-resolution mass spectral data with those in the literature (16). The chromatographic fingerprint is shown in Fig.…”