Development of a bispecific monoclonal antibody for use in molecular hybridisation

Auriol, Jacques; Guesdon, Jean‐Luc; Mazié, J. C.; Nato, Farida

doi:10.1016/0022-1759(94)90131-7

Cited by 13 publications

(4 citation statements)

References 28 publications

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“…Since individual light chains and heavy chains are transcribed separately and assembled randomly, the desired bsMAb produced by the quadroma could range 10-50% (Suresh et al, 1986a). Hence, purification of this bsMAb is essential and has been achieved by hydroxylapatite chromatography (Karawajew et al, 1987;De Lau et al, 1992), ion-exchange chromatography (Suresh et al, 1986b;Auriol et al, 1994) double affinity chromatography (Sahin et al, 1990;Smith et al, 1992), mimetic affinity methods (Xu et al, 1998), and gradient thiophilic T-gel chromatography (Kreutz et al, 1998b).…”

Section: Production Of Bispecific Antibodiesmentioning

confidence: 99%

Bispecific Antibodies as Novel Bioconjugates

Cao

Suresh

1998

Bioconjugate Chem.

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Bispecific antibodies are unique macromolecular heterobifunctional cross-linkers with two different binding specificities within a single molecule. As ideal bioconjugates, they can specifically glue any two different molecules together without the need for chemical conjugation. With this unique feature, they have immense potential in biological and immunological fields. Their applications range from immunohistochemistry, immunoassays, radioimmunodiagnosis, radioimmunotherapy, and immunotherapy. Recently, a new second generation of bispecific molecules, bispecific single chain Fv and diabodies, has been produced by DNA recombinant technology. They can be considered as the ultimate magic bullets for in vivo applications. They may theoretically improve tumor or pathogen targeting and minimize side effects, eventually replacing the full-length bispecific antibodies. Emphasizing on developmental methodology and clinical applications of bispecific antibodies, this review gives a bird's-eye view of these unique bioconjugates.

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Section: Production Of Bispecific Antibodiesmentioning

confidence: 99%

Bispecific Antibodies as Novel Bioconjugates

Cao

Suresh

1998

Bioconjugate Chem.

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“…The hybridized probe was detected using the bispecific antibody (Bi-Mab) anti-AAF and antialkaline phosphatase as described elsewhere [16]. Membranes were incubated for 2 h with a mixture of 1 pg ml-' of Bi-Mab and 1 pg ml-' of alkaline phosphatase (Boehringer) in Tris-buffer pH 8 containing 0.15 M NaCl, 0.1% Tween 20 (TBS-Tw) and 1% BSA.…”

Section: Immunodetection Of Dna-rrna Duplexesmentioning

confidence: 99%

“…Membranes were incubated for 2 h with a mixture of 1 pg ml-' of Bi-Mab and 1 pg ml-' of alkaline phosphatase (Boehringer) in Tris-buffer pH 8 containing 0.15 M NaCl, 0.1% Tween 20 (TBS-Tw) and 1% BSA. They were then washed three times for 10 min with TBS-Tw, and enzymatic activity was detected using a mixture of nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (Boehringer) as described elsewhere [16,18].…”

Section: Immunodetection Of Dna-rrna Duplexesmentioning

confidence: 99%

“…The aim of our work was to study two populations of N. meningitidk, one originating in France and the other in the Central African Republic, using ribotyping and PCR. A non-radioactive probe label was used for ribotyping; detection consisted of an immunoenzymatic procedure recently described [16]. The PCR procedure was modified slightly to differentiate between subpopulations in serogroup A.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of serogroup A Neisseria meningitidis strains by rRNA gene restriction patterns and PCR: Correlation with the results of serotyping, subtyping and multilocus enzyme electrophoresis

Auriol

1995

FEMS Immunology and Medical Microbiology

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We studied 35 strains of Neisseria meningitidis serogroup A from different locations (France, Central African Republic, Sudan and Burkina Faso) using both ribotyping and a polymerase chain reaction (PCR). A non-radioactive probe label was used for ribotyping; detection consisted of an immunoenzymatic procedure using a bispecific antibody. The PCR was designed to amplify the 16S-23S rDNA internal transcribed spacer. These techniques were compared with other markers. The strains were identified as belonging to three clones (I, III-1, IV) by multilocus enzyme electrophoresis (MEE) and to three subtypes by serological methods. Ribotyping identified five groups and PCR identified four groups. Ribotyping gave more diversity between strains than either MEE or sero/subtyping, but confirmed the epidemiological data provided by the combination of these two techniques. The PCR provided a simple and convenient one-step procedure for the differentiation of strains of serogroup A.

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