Development, characterization, and toxicity evaluation of amphotericin B–loaded gelatin nanoparticles

Nahar, Manoj; Mishra, Dinesh Kumar; Dubey, Vaibhav; Jain, Narendra Kumar

doi:10.1016/j.nano.2008.03.007

Cited by 141 publications

(85 citation statements)

References 32 publications

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“…The toxicity of NP drug delivery systems has been a prominent concern over the past 10 years (Nahar et al, 2008;Jain et al, 2011). Related studies have shown that NPs enhance therapeutic effects but can also increase toxicity.…”

Section: Mtt Assaymentioning

confidence: 99%

Preparation and in vitro evaluation of thienorphine-loaded PLGA nanoparticles

Yang¹,

Xie

Mei³

2014

Drug Delivery

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Poly (D,L-lactic-co-glycolide) nanoparticles (PLGA-NPs) have attracted considerable interest as new delivery vehicles for small molecules, with the potential to overcome issue such as poor drug solubility and cell permeability. However, their negative surface charge decreases bioavailability under oral administration. Recently, cationically modified PLGA-NPs has been introduced as novel carriers for oral delivery. In this study, our aim was to introduce and evaluate the physiochemical characteristics and bioadhesion of positively charged chitosancoated PLGA-NPs (CS-PLGA-NPs), using thienorphine as a model drug. These results indicated that both CS-PLGA-NPs and PLGA-NPs had a narrow size distribution, averaging less than 130 nm. CS-PLGA-NPs was positively charged (+42.1 ± 0.4 mV), exhibiting the cationic nature of chitosan, whereas PLGA-NPs showed a negative surface charge (À2.01 ± 0.3 mV). CS-PLGA-NPs exhibited stronger bioadhesive potency than PLGA-NPs. Furthermore, the transport of thienorphine-CS-PLGA-NPs by Caco-2 cells was higher than thienorphine-PLGA-NPs or thienorphine solution. CS-PLGA-NPs were also found to significantly enhance cellular uptake compared with PLGA-NPs on Caco-2 cells. An evaluation of cytotoxicity showed no increase in toxicity in either kind of nanoparticles during the formulation process. The study proves that CS-PLGA-NPs can be used as a vector in oral drug delivery systems for thienorphine due to its positive surface charge and bioadhesive properties.

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Section: Mtt Assaymentioning

confidence: 99%

Preparation and in vitro evaluation of thienorphine-loaded PLGA nanoparticles

Yang¹,

Xie

Mei³

2014

Drug Delivery

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“…Since almost similar results were found with two methods, the direct method was applied for estimation of drug in nanoparticles. The encapsulation efficiency and the percent loading of ethionamide were then calculated as follows (Nahar et al, 2008): Ethionamide-loaded nanoparticles were then stored in 5 ml glass vials sealed with plastic caps and were kept in a refrigerator with a temperature of 4-8°C. The physical stability of drug-loaded nanoparticles was measured before and after 90 days storage.…”

Section: Characterization Of Drug-loaded Plga Nanoparticlesmentioning

confidence: 99%

Pharmacokinetics and tissue distribution studies of orally administered nanoparticles encapsulated ethionamide used as potential drug delivery system in management of multi-drug resistant tuberculosis

et al. 2010

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“…Amphotericin B (AmB) is a potent polyene macrolide as broad-spectrum antibiotic to treat life-threatening systemic and local fungal infections with restricted therapeutic efficacy owing to poor water solubility and severe nephrotoxicities (Kleinberg, 2006;Nahar et al, 2008;Shafaat et al, 2013). The conventional commercial dosage form (Fungizone Õ ) is available as a micellar suspension using the sodium deoxycholate as bile salt (Wong-Beringer et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Formulation and optimization of nanoemulsion using antifungal lipid and surfactant for accentuated topical delivery of Amphotericin B

Hussain

Singh²,

Singh³

et al. 2016

Drug Delivery

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The objective of the study was to develop, optimize and evaluate a nanoemulsion (NE) of Amphotericin B (AmB) using excipients with inherent antifungal activities (Candida albicans and Aspergillus niger) for topical delivery. AmB-loaded NE was prepared using Capmul PG8 (CPG8), labrasol and polyethylene glycol-400 by spontaneous titration method and evaluated for mean particle size, polydispersity index, zeta potential and zone of inhibition (ZOI). NE6 composed of CPG8 (15%w/w), S mix (24%w/w) and water (61%w/w) was finally selected as optimized NE. AmB-NE6 was studied for improved in vitro release, ex vivo skin permeation and deposition using the Franz diffusion cell across the rat skin followed with drug penetration using confocal laser scanning microscopy (CLSM) as compared to drug solution (DS) and commercial Fungisome Õ . The results of in vitro studies exhibited the maximum ZOI value of NE6 as 19.1 ± 1.4 and 22.8 ± 2.0 mm against A. niger and C. albicans, respectively, along with desired globular size (49.5 ± 1.5 nm), zeta potential (À24.59 mV) and spherical morphology. AmB-NE6 revealed slow and sustained release of AmB as compared to DS in buffer solution (pH 7.4). Furthermore, AmB-NE6 elicited the highest flux rate (22.88 ± 1.7 mg/cm 2 /h) as compared to DS (2.7 ± 0.02 mg/cm 2 /h) and Fungisome Õ (11.5 ± 1.0 mg/cm 2 /h). Moreover, the enhancement ratio and drug deposition were found to be highest in AmB-NE6 than DS across the stratum corneum barrier. Finally, CLSM results corroborated enhanced penetration of the AmB-NE6 across the skin as compared to Fungisome Õ and DS suggesting an efficient, stable and sustained topical delivery.

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Development, characterization, and toxicity evaluation of amphotericin B–loaded gelatin nanoparticles

Cited by 141 publications

References 32 publications

Preparation and in vitro evaluation of thienorphine-loaded PLGA nanoparticles

Preparation and in vitro evaluation of thienorphine-loaded PLGA nanoparticles

Pharmacokinetics and tissue distribution studies of orally administered nanoparticles encapsulated ethionamide used as potential drug delivery system in management of multi-drug resistant tuberculosis

Formulation and optimization of nanoemulsion using antifungal lipid and surfactant for accentuated topical delivery of Amphotericin B

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