Animal Cell Culture and Production of Biologicals 1991
DOI: 10.1007/978-94-011-3550-4_4
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Cited by 3 publications
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“…All human cell lines were incubated at 37 °C in a 5% CO 2 atmosphere and maintained under sterile conditions [43]. Cells were tested for Mycoplasma (Lonza, Alpharetta, GA, USA) using the manufacturer’s conditions prior to experiments.…”
Section: Methodsmentioning
confidence: 99%
“…All human cell lines were incubated at 37 °C in a 5% CO 2 atmosphere and maintained under sterile conditions [43]. Cells were tested for Mycoplasma (Lonza, Alpharetta, GA, USA) using the manufacturer’s conditions prior to experiments.…”
Section: Methodsmentioning
confidence: 99%
“…Cells were maintained and sub-cultured according to ATCC recommendations without any antibiotics. The absence of mycoplasma was verified by fluorescence detection according to Hay et al [23]. For transfection, HEK 293 cells were seeded in 24-well culture plates using 500 μl/well of complete medium and 50,000 cells/well incubated at 37°C, 5% CO 2 .…”
Section: Methodsmentioning
confidence: 99%