Eur. J. Chem. volume 5, issue 2, P219-226 2014 DOI: 10.5155/eurjchem.5.2.219-226.966
Ibrahim Ahmed Naguib, Eglal Abdelhamid Abdelaleem, Hala Elsayed Zaazaa, Essraa Abd ElWahab Hussein
Abstract: Three simple and sensitive methods were developed for the determination of acemetacin (ACM) in presence of its degradation product, indomethacin (DEG). Several methods were evaluated and are presented. Method A was based on measuring the peak amplitude of the first derivative of the ratio spectra 1 DD at 244 nm. In method B, mean centering of ratio spectra method (MCR) was applied, which depends on measuring the mean centered values of ratio spectra of ACM at 234 nm. Method C provided separation of ACM from its DEG on preactivated silica gel 60F254 HPTLC plates using hexane:ethyl acetate:glacial acetic acid (6:4:0.3, v:v:v) as developing system followed by scanning at 254 nm. The suggested methods were validated in compliance with the International Conference on Harmonisation (ICH) guidelines and were successfully applied for quantification of ACM in its commercial capsule. The proposed methods were also statistically compared to a reported HPLC method with no significant difference in performance; indicating the ability of the proposed method to be reliable and suitable for routine analysis of drug product. HPTLC Validation Acemetacin Degradation First derivative of ratio spectra Mean centering of ratio spectra
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