Development and validation of RP-HPLC, HPTLC and UV-visible spectrophotometric methods for simultaneous estimation of alprazolam and propranolol hydrochloride in their combined dosage form

Patel, Divyesh; Patel, N. R.; Sherikar, Omkar; Mehta, Priti

doi:10.1134/s1061934814070119

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…Literature survey suggests that few analytical methods have been reported for the estimation of diazepam and propranolol hydrochloride, individually and with other drugs by spectrophotometry [1][2] and by liquid chromatography [3][4][5][6] capillary electrophoresis 7, GC 8, HPTLC 9 The method developed shows short run time, with good resolution, theoretical plates and was found to be economical than the other research works. The aim of the present study is to develop a simple, precise, accurate, sensitive HPLC method for the estimation of diazepam and propranolol hydrochloride in tablet dosage form.…”

Section: Figure 2: Structure Of Diazepammentioning

confidence: 99%

A Precise Rp-HPLC Method Development for the Simultaneous Estimation of Diazepam and Propranolol Hydrochloride in Tablet Dosage Form

Saravanan¹,

Shaik²

2012

J. Drug Delivery Ther.

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Section: Figure 2: Structure Of Diazepammentioning

confidence: 99%

A Precise Rp-HPLC Method Development for the Simultaneous Estimation of Diazepam and Propranolol Hydrochloride in Tablet Dosage Form

Saravanan¹,

Shaik²

2012

J. Drug Delivery Ther.

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“…Gramocef L® is the marketed combined tablet formulation brand of LIN and CEF in the ratio of 600 mg: 200 mg respectively. Concurrent analysis of CEF and LIN in a fixed dose form was reported using HPLC methods [ 23 – 30 ], various spectrophotometric methods, including Vierodt’s method [ 31 – 35 ], the absorbance ratio method (Q analysis) [ 26 , 32 , 36 , 37 ], first order derivative spectrophotometry [ 38 ], zero crossing second derivative spectrophotometry [ 34 , 36 ], ratio derivative method [ 34 , 39 ], ratio difference method [ 39 ] and mean centering of ratio spectra [ 39 ]. To the extent that we are aware, this is the first electro-driven separation method utilizing capillary electrophoresis for concurrent analysis of the two cited drugs.…”

Section: Introductionmentioning

confidence: 99%

Development, validation and greenness assessment of a new electro-driven separation method for simultaneous analysis of cefixime trihydrate and linezolid in their fixed dose combination

Habeeb,

Morshedy,

Daabees

et al. 2023

BMC Chemistry

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The establishment and validation of a straightforward, accurate, and eco-friendly capillary zone electrophoretic-diode array detection (CZE-DAD) procedure has been presented for concurrent measurement of two common antibiotics, namely, linezolid (LIN) and cefixime trihydrate (CEF), in their binary mixture or combined dosage form. The selected fused silica capillary has total and effective lengths equal to 58.5 cm and 50 cm, respectively, with a 50 µm internal diameter. Injections were performed utilizing 100 mM borate buffer at pH 10.2 as the background electrolyte (BGE) with a 15.0 s injection time. The finally utilized voltage was 30 kV. DAD was programmed to measure LIN at 250 nm and CEF at 285 nm. In less than 6 min, the two cited drugs were resolved at 2.51 and 5.47 min for LIN and CEF respectively. The introduced procedure had a linear response in the concentration range of 5–50 μg/mL for both analytes with correlation coefficients > 0.9999. Detection and quantification limits were 1.213 and 4.042 μg/mL, respectively, for LIN and 0.301 and 1.004 μg/mL, respectively, for CEF. Validation was conducted according to the International Council for Harmonization (ICH), concerning linearity, detection and quantitation limits, range, accuracy, precision, selectivity, and robustness. Precision was found acceptable due to the low relative standard deviation (RSD%) values that did not exceed 1.86% either for repeatability or for intermediate precision. Additionally, the adequately recovered concentrations and the low values of percentage relative error (Er%) provide evidence of the accuracy of the proposed method. On the other hand, the robustness of the introduced method was affirmed by the acceptable RSD% values that did not exceed 0.6% after deliberate changes in the following procedure parameters: buffer concentration, buffer pH, and wavelength. Finally, the ability of the presented method to quantify the two tested drugs in laboratory-prepared tablets was confirmed by the adequate recoveries (≥ 99%) utilizing the standard-addition procedure, along with the absence of any significant difference between the proposed method and the reference method as proven by the student’s t-test and the variance-ratio F-test values that did not exceed the theoretical ones. The analytical Eco-Scale and the analytical GREEness metric (AGREE) were the tools utilized for greenness assessment. This CZE procedure is the first electro-driven separation method that was utilized for the analysis of both antibiotics in their combined laboratory-prepared tablets with no interference from the co-formulated adjuvants.

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“…Flunarizine dihydrochloride is official in BP 6 . Literature survey reveals that some methods have been developed for their determination by HPLC [7][8][9] or spectrophotometry [10][11][12] either alone or in combination, but no method was found for the selected combined dosage form by first order derivative UV spectrophotometry. Derivative spectrophotometry [13][14][15] is one of the advanced spectrophotometric techniques useful for determination of quantitative and qualitative analysis.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of First Order Derivative Uv Spectrophotometric Method for Simultaneous Estimation of Propranolol Hydrochloride and Flunarizine Dihydrochloride in Bulk and Combined Dosage Form

Wagh¹,

Deshmukh²,

Vassa³

et al. 2013

Int. Res. J. Pharm.

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The first order derivative of UV spectrometry method for simultaneous determination of Propranolol hydrochloride (PRO) and Flunarizine dihydrochloride (FLU) in pure bulk drug and combined dosage form was found to be simple, accurate, fast, precise and reproducible. The first derivative values measured at 289nm for PRO and 253nm for FLU. The linearity for zero order derivative method was carried out by using the concentration range 4-28µg/ml for PRO and 3-7µg/ml for FLU. The coefficient correlation of PRO and FLU for zero order was found to be 0.9995 and 0.9991 respectively. At zero crossing point of PRO (289nm) FLU showed a measurable derivative absorbance where as at the zero crossing point of FLU (253nm), PRO showed appreciable derivative absorbance value. The coefficient correlation of PRO and FLU for first order derivative was found to be 0.9991 and 0.9995 respectively. Precision study showed that % RSD was within the range of acceptable limits (<2). The % recovery for PRO and FLU was found to be in the range of 98-102% and 100-101% respectively. The percentage assay was found to be as 99.5 and 100.12% for PRO and FLU. The results of analysis have been validated as per ICH Q2 (R1) guidelines. This method has applied successfully for the determination of PRO and FLU in its combination with a high percentage of recovery good accuracy and precision. . Development and validation of first order derivative UV Spectrophotometric method for simultaneous estimation of Propranolol hydrochloride and Flunarizine dihydrochloride in bulk and combined dosage form. Int. Res. J.

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Development and validation of RP-HPLC, HPTLC and UV-visible spectrophotometric methods for simultaneous estimation of alprazolam and propranolol hydrochloride in their combined dosage form

Cited by 8 publications

References 12 publications

A Precise Rp-HPLC Method Development for the Simultaneous Estimation of Diazepam and Propranolol Hydrochloride in Tablet Dosage Form

A Precise Rp-HPLC Method Development for the Simultaneous Estimation of Diazepam and Propranolol Hydrochloride in Tablet Dosage Form

Development, validation and greenness assessment of a new electro-driven separation method for simultaneous analysis of cefixime trihydrate and linezolid in their fixed dose combination

Development and Validation of First Order Derivative Uv Spectrophotometric Method for Simultaneous Estimation of Propranolol Hydrochloride and Flunarizine Dihydrochloride in Bulk and Combined Dosage Form

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