Development and validation of high-throughput liquid chromatography–tandem mass spectrometric method for simultaneous quantification of loratadine and desloratadine in human plasma

Gedela, Srinubabu; Patel, Rajaram S.; Shedbalkar, V. P.; Rao, Allam Appa; Rao, M. Narasimha; Bandaru, Veera Venkata Ratnam

doi:10.1016/j.jchromb.2007.10.036

Cited by 22 publications

(9 citation statements)

References 27 publications

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“…On the other hand, the British Pharmacopoeia (3) describes a potentiometic titration method, using perchloric acid as a titrant, for the determination of LRT in raw materials. A literature survey revealed that various methods have been published for the determination of LRT in pharmaceuticals and biological fluids, including spectrophotometry (4)(5)(6)(7)(8)(9)(10)(11), spectrofluorimetry (8), atomic absorption spectrometry (11), liquid chromatography (5,7,9,(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22), gas chromatography (23), densitometry (9), capillary electrophoresis (24) and polarography (25).…”

Section: Introductionmentioning

confidence: 99%

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Stability‐indicating micelle‐enhanced spectrofluorimetric method for determination of loratadine and desloratadine in dosage forms

et al. 2011

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A highly sensitive and simple spectrofluorimetric method was developed for the determination of loratadine (LRT) and desloratadine (DSL) in their pharmaceutical formulations. The proposed method is based on investigation of the fluorescence spectral behaviour of LRT and DSL in a sodium dodecyl sulphate (SDS) micellar system. In aqueous solution of acetate buffer of pH 4.5, the fluorescence intensities of both LRT and DSL were greatly enhanced (240%) in the presence of SDS. The fluorescence intensity was measured at 438 nm after excitation at 290 nm for both drugs. The fluorescence-concentration plots were rectilinear over the range 0.05-2.0 µg/mL for both LRT and DSL, with lower detection limits of 5.13 × 10(-3) and 6.35 × 10(-3) µg/mL for LRT and DSL, respectively. The method was successfully applied to the analysis of the two drugs in their commercial tablets, capsules and syrups, and the results were in good agreement with those obtained with the official or comparison methods. The proposed method is specific for the determination of LRT in the presence of other co-formulated drugs, such as pseudoephedrine. The application of the proposed method was extended to stability studies of LRT and DSL after exposure to different forced degradation conditions, such as acidic, alkaline and oxidative conditions, according to ICH guidelines.

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Section: Introductionmentioning

confidence: 99%

“…Regarding DSL, several methods have been derived for its determination, such as spectrophotometry (26,27), spectrofluorimetry (27), liquid chromatography (12)(13)(14)(15)(16)(17)(18)(19)(20)(27)(28)(29), gas chromatography (23) and capillary isotachophoresis (30).…”

Section: Introductionmentioning

confidence: 99%

Stability‐indicating micelle‐enhanced spectrofluorimetric method for determination of loratadine and desloratadine in dosage forms

et al. 2011

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“…In some articles, the abbreviation HTLC was used for completely different techniques-high-throughput liquid chromatography [18,19], or high turbulence liquid chromatography [20], the latter actually referring to turbulent-flow chromatography (a sample preparation step for biological samples using a narrow-bore, large particle size column at high flow-rates, see Section 3.3.7). This could cause confusion, especially in the use of scientific literature databases.…”

Section: High Temperature Liquid Chromatography (Htlc)mentioning

confidence: 99%

“…TFC has been recently employed in the analysis of loratadin and desloratadin in human plasma [19], sitagliptin in human urine and hemodialysate [258], immunosuppressants in blood [259], antidepressants in serum [260], dextromethorphan and its metabolites in plasma [261,262] and some others [225].…”

Section: Turbulent-flow Chromatography (Tfc)mentioning

confidence: 99%