Development and Validation of an UV Spectrophotometric Method for Determination of Thiamphenicol in Dosage Form

Martins, Yugo Araújo; Oliveira, Cristiani Lopes Capistrano Gonçalves de

doi:10.1007/s10812-019-00870-w

Cited by 14 publications

(12 citation statements)

References 20 publications

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“…The proposed UV method for the estimation of paliperidone palmitate was validated in terms of parameters like linearity, range, precision, robustness, ruggedness, accuracy and sensitivity. The method was validated as per the guidelines of the International Conference on Harmonization (ICH) [19][20][21].…”

Section: Methods Validationmentioning

confidence: 99%

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Development and Validation of Uv Spectroscopic Method of Paliperidone Palmitate in Bulk and Its Application for Drug Loading and Entrapment Efficiency Analysis of Long-Acting Injectable Microspheres

Mali

Oza

2021

Int J App Pharm

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Objective: The objective of the current study was to develop simple, precise and cost-effective ultraviolet (UV) spectroscopic method for estimation of paliperidone palmitate (PP) as a bulk and in the polymeric depot dosage form. Methods: UV spectrophotometer with quartz sample cells and spectral manager software was used for analysis samples. 20 % Methanol in Mili-Q-Water was used as a solvent for the sample and standard preparations. Method validation was carried out as per ICH Q2 (R1) guidelines. Stress degradation studies were carried out to check the stability-indicating performance of the developed method. The validated method was applied for the estimation of the PP content of long-acting injectable microspheres of PP. Results: The wavelength of maximum absorption was found to be 283 nm. The proposed method was validated in the range of 5-30 µg/ml of PP. The mean recovery for 80%, 100% and 120% standard solution was found to be 99.03%, 99.21% and 99.35% respectively with less than 2% relative standard deviation (RSD). Intra-day and inter-day method precision ranged from 0.54 to 1.62% and 0.67 and 1.24% respectively. Limit of detection (LOD) and limit of quantitation (LOQ) were estimated to be 0.03 and 0.10 µg/ml respectively. The developed method was found to be robust and rugged. Stress degradation studies were carried out under acidic, alkaline, hydrolytic and oxidative and photo stress. The proposed method was capable to detect changes in assay due to stress conditions. Conclusion: The developed method was successfully employed for routine analysis of drug loading in long-acting injectable microspheres of PP.

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Section: Methods Validationmentioning

confidence: 99%

“…The sensitivity of measurements of PP by the use of the proposed method was estimated in terms of the limit of detection (LOD) and limit of quantitation (LOQ) [20,21]. The sensitivity data of the study were shown in table 7.…”

Section: Sensitivitymentioning

confidence: 99%

Development and Validation of Uv Spectroscopic Method of Paliperidone Palmitate in Bulk and Its Application for Drug Loading and Entrapment Efficiency Analysis of Long-Acting Injectable Microspheres

Mali

Oza

2021

Int J App Pharm

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“…Around 50 mg of moclobemide was carefully weighed and taken in a volumetric flask of 50 ml capacity, where it was dissolved in 40 ml of Milli-Q® water. The volume was adjusted up to the mark with Milli-Q® water to make a 1000 μg/ml stock solution (Jadhav et al, 2014;Martins, Oliveria, 2019).…”

Section: Preparation Of Standard Stock Solution Of Moclobemidementioning

confidence: 99%

“…The absorbances of solutions were measured at 239 nm by using a UV spectrophotometer. Milli-Q® water was used as blank for measurement (Martins, Oliveria, 2019;Maleque et al, 2012;Jenke, 1996;Jadhav et al, 2011). The linearity of solution preparation was presented in table 2.…”

Section: Linearitymentioning

confidence: 99%

Stability indicating method development and validation of moclobemide in its pharmaceutical dosage forms by UV-spectrophotometric method

Mahapatra¹,

Paul²,

Gupta³

et al. 2022

Adv Pharm J

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Objective: The present study describes a simple, sensitive, fast, accurate, and cost-effective UV (Ultraviolet) spectrophotometric method for the determination of moclobemide in its various pharmaceutical dosage forms. Materials and methods: This UV spectrophotometric method is used the first time and has been applied for the quantification and determination of moclobemide drug substances in pharmaceutical formulations with suitable validation parameters and stability studies.A stable, precise, and stability-indicating ultraviolet spectrophotometric method was developed Results: for the analysis of the moclobemide in the presence of the degradation products. It involves method development by validation and force degradation studies; in which distilled water, hydrochloric acid as acidic environment, sodium hydroxide as basic treatment, hydrogen peroxide as oxidation agent were used as solvents. Two methods (method A and method B) were developed. Method A involves for method development and validation studies whereas method B involves forced degradation studies. Beer's law was followed and showed peak maxima at 239 nm wavelength. The method was validated for linearity, accuracy, precision, sensitivity, specificity, recovery, robustness, and ruggedness as per the ICH guideline. The results of the forced degradation study also indicated that the developed ultraviolet method is capable of detecting percent degradation in different stress conditions, which signifies that the developed method is robust and stable.Statistical analysis of the data showed that the proposed UV spectrophotometric method is novel, Conclusions: reproducible, accurate, and precise while being simple and rapid for the determination of moclobemide in bulk drugs and its pharmaceutical formulations.

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“…Hence, the European Economic Community (EEC) [ 6 ] has defined the maximum residue limit (MRL) of TAP in milk and poultry muscle tissues as 50 µg·kg −1 . Currently, the conventional detection methods for TAP involve enzyme-linked immunosorbent (ELISA) [ 7 ] assay, UV spectrophotometry [ 8 ], high-performance liquid chromatography (HPLC) [ 9 ], and high-performance liquid chromatography–mass spectrometry (LC-MS) [ 10 ]. Among these, ELISA can rapidly achieve trace detection and cope with large number of samples but is still prone to false positives or negatives.…”

Section: Introductionmentioning

confidence: 99%

Facile Synthesis of N, S-Doped Carbon Quantum Dots from Food Waste as Fluorescent Probe for Sensitive Detection of Thiamphenicol and Its Analogues in Real Food Samples along with an Application in Bioimaging

Chen

Ouyang

Zhu

et al. 2022

Foods

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Herein, N, S co-doped carbon quantum dots (N, S-CDs) with high absolute quantitative yield (Abs-QY) of 50.2% were produced by hydrothermal treatment of food residue crayfish shells. A new detection method of thiamphenicol (TAP) and its analogues was established by discovering the obvious fluorescence response between TAP and N, S-CDs, which achieved a wide linear range of 20–300 μg·L−1 with a detection limit (LOD) of 11.12 μg·L−1. This novel probe exhibited strong sensitivity and shows rapid response in complex food matrices (overall detection time is less than 45 min) mainly induced by static quenching. Spiked food sample recovery ranged from 97.3 to 99.34%. Further, the cell experiments of N, S-CDs were conducted, and the cell viability remained 91.76% under high concentration of N, S-CDs due to the environmentally friendly materials. The low cytotoxicity and good cytocompatibility make these N, S-CDs compatible for cell bioimaging and intracellular detection of TAP.

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Development and Validation of an UV Spectrophotometric Method for Determination of Thiamphenicol in Dosage Form

Cited by 14 publications

References 20 publications

Development and Validation of Uv Spectroscopic Method of Paliperidone Palmitate in Bulk and Its Application for Drug Loading and Entrapment Efficiency Analysis of Long-Acting Injectable Microspheres

Development and Validation of Uv Spectroscopic Method of Paliperidone Palmitate in Bulk and Its Application for Drug Loading and Entrapment Efficiency Analysis of Long-Acting Injectable Microspheres

Stability indicating method development and validation of moclobemide in its pharmaceutical dosage forms by UV-spectrophotometric method

Facile Synthesis of N, S-Doped Carbon Quantum Dots from Food Waste as Fluorescent Probe for Sensitive Detection of Thiamphenicol and Its Analogues in Real Food Samples along with an Application in Bioimaging

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