Development and Optimization of Terpene-Enriched Vesicles (Terpesomes) for Effective Ocular Delivery of Fenticonazole Nitrate: In vitro Characterization and in vivo Assessment

Albash, Rofida; Al-mahallawi, Abdulaziz Mohsen; Hassan, Mariam; Alaa-Eldin, Ahmed Adel

doi:10.2147/ijn.s274290

Cited by 34 publications

(55 citation statements)

References 41 publications

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“…The superiority of MOX LPs in the in-vivo corneal infection model might also be attributed to the nature of the LPs and smaller PS that contributed to better corneal retention with more effective corneal contact and enhanced MOX bioavailability. Our findings comply with previous studies which have reported that drug entrapment into nanoparticles enhanced drug pharmacokinetics in the aqueous humor, 6 , 8 , 46 promoting the application of LPs as an effective ocular delivery system. It is worth noting that the existence of CTAB enhanced the antibacterial effect of MOX-loaded LPs compared to MOX solution as it has an antibacterial effect, as previously reported.…”

Section: Resultssupporting

confidence: 93%

“…These results indicate that the prepared TPs were polydisperse but within acceptable limits. 6 It was found that only terpene type (X 2 ) significantly affects PDI, at a p-value =0.0188 in which a direct correlation was detected between the PS and the PDI where the highest PDI value was found in vesicles that had the largest PS, and this is in agreement with Tawfik et al 35 . While regarding LPs formulations, PDI values ranged from 0.26±0.005 to 0.55±0.01 as displayed in Table 3 and Figure 2C .…”

Section: Resultssupporting

confidence: 81%

“…5 Terpesomes (TPs) are terpenes containing vesicles, previously described by Albash et al, to treat ocular candidiasis. 6 Terpenes are naturally occurring substances derived from essential oils and made up of several isoprene units. 6 Terpenes are not only well-known penetration enhancers, but they also possess both antimicrobial and antifungal activities as a consequence of their residence in the lipophilic entities of the cell bilayer; which allows constituents of essential oils to be delivered to the inner of the cell, leading to cytoplasmic infiltration, and cell death.…”

Section: Introductionmentioning

confidence: 99%

“…6 Terpenes are naturally occurring substances derived from essential oils and made up of several isoprene units. 6 Terpenes are not only well-known penetration enhancers, but they also possess both antimicrobial and antifungal activities as a consequence of their residence in the lipophilic entities of the cell bilayer; which allows constituents of essential oils to be delivered to the inner of the cell, leading to cytoplasmic infiltration, and cell death. 7 On the other hand, Leciplex is a new vesicular nanosystem based on the combination of negatively charged phospholipids, cationic surfactants, and Transcutol ® .…”

Section: Introductionmentioning

confidence: 99%

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Tailoring Terpesomes and Leciplex for the Effective Ocular Conveyance of Moxifloxacin Hydrochloride (Comparative Assessment): In-vitro, Ex-vivo, and In-vivo Evaluation

Albash¹,

Abdellatif²,

Hassan³

et al. 2021

IJN

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Aim To compare the ability of both terpesomes (TPs) and leciplex (LPs) loaded moxifloxacin hydrochloride (MOX) for enhancing ocular drug conveyance. Methods Two separate 2 1 .3 1 full-factorial trials were established to determine the influence of multiple variables upon nanovesicles properties and select the optimized formulae using Design Expert ® software. The thin-film hydration method was used to formulate TPs, while the single-step procedure was used for LPs. All formulae were characterized for their entrapment efficiency percent (EE%), particle size distribution (PS), polydispersity index (PDI), and zeta potential (ZP). Then, the optimized formulae were selected, evaluated, and compared for additional assessments. Results The optimized formulae TP4 and LP1 showed EE% of 84.14±0.21 and 78.47±0.17%, PS of 578.65±5.65 and 102.41±3.39 nm, PDI of 0.56±0.04 and 0.28±0.01, ZP of −12.50±0.30 and 32.50±0.50 mV, respectively. Further, LP1 showed enhanced corneal permeation across cow cornea compared to MOX solution and TP4. Besides, confocal laser scanning microscopy assessment viewed valuable infiltration from the fluoro-labeled LP through corneal layers compared to TP. LP1 showed spherical morphology and, its ability to adhere to mucus membranes was justified. Further, LP1 showed superiority over MOX solution in biofilm inhibition and eradication in addition to the treatment of infected mice with methicillin-resistant Staphylococcus aureus without any inflammatory response. Finally, the histopathological study verified the harmlessness and biocompatibility of the assembled LPs. Conclusion The gained outcomes confirmed the capability of utilizing LPs as a successful nanovesicle for the ocular conveyance of MOX over TPs and MOX solution.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tailoring Terpesomes and Leciplex for the Effective Ocular Conveyance of Moxifloxacin Hydrochloride (Comparative Assessment): In-vitro, Ex-vivo, and In-vivo Evaluation

Albash¹,

Abdellatif²,

Hassan³

et al. 2021

IJN

Self Cite

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“…Each preparation was appropriately diluted using distilled water (1:10 v/v ) to measure the PS and PDI. All measurements were done in triplicates at 25 °C [ 46 , 47 , 48 ].…”

Section: Methodsmentioning

confidence: 99%

Investigation of the Potential of Nebivolol Hydrochloride-Loaded Chitosomal Systems for Tissue Regeneration: In Vitro Characterization and In Vivo Assessment

et al. 2021

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In this study, we evaluated the synergistic effect of nebivolol hydrochloride (NVH), a third-generation beta-blocker and NO donor drug, and chitosan on the tissue regeneration. Ionic gelation method was selected for the preparation of NVH-loaded chitosomes using chitosan lactate and sodium tripolyphosphate. The effect of different formulation variables was studied using a full factorial design, and NVH entrapment efficiency percentages and particle size were selected as the responses. The chosen system demonstrated high entrapment efficiency (73.68 ± 3.61%), small particle size (404.05 ± 11.2 nm), and good zeta potential value (35.6 ± 0.25 mV). The best-achieved formula demonstrated spherical morphology in transmission electron microscopy and amorphization of the crystalline drug in differential scanning calorimetry and X-ray diffraction. Cell culture studies revealed a significantly higher proliferation of the fibroblasts in comparison with the drug suspensions and the blank formula. An in vivo study was conducted to compare the efficacy of the proposed formula on wound healing. The histopathological examination showed the superiority of NVH-loaded chitosomes on the wound proliferation and the non-significant difference in the collagen deposition after 15 days of the injury to that of intact skin. In conclusion, NVH-loaded chitosomes exhibited promising results in enhancing skin healing and tissue regeneration.

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Carbon Nanotube Hybrid Materials: Efficient and Pertinent Platforms for Antifungal Drug Delivery

Chandra,

Reis,

Kundu

et al. 2023

Adv Materials Technologies

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Carbon nanotubes (CNTs) have emerged as the brightest nascent artifact to deliver antifungal drugs in drug delivery applications, health care, and pharmaceutical industries. Excellent physio‐chemical features such as huge surface area, tunable side wall, and microneedle‐like morphology make CNTs suitable for drug carriers. Chemical attachments (covalent and non‐covalent functionalization) result in the formation of functionalized CNTs (F‐CNTs) and CNT‐based hybrid materials (CNT‐HMs). These F‐CNTs and CNT‐HMs have substantial antifungal activity and also have the potential to immobilize antifungal drugs such as amphotericin B, nystatin, curcumin, etc. on the exterior or interior surface, securely transport to the target sites, permeate through bio‐barriers, and release these drugs in a controlled manner. As antifungal drug carriers, F‐CNT and CNT‐HMs exhibit more excellent antifungal activity than other conventional drug delivery systems and have the potency to invade biofilm to circumvent the multidrug resistance of fungal species. This review focuses on CNTs and CNT‐HMs for antifungal drug delivery, including their functionalization methods, drug loading approaches, drug release mechanism, cellular internalization, delivery efficiency, and cellular toxicities with their workaround.

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Development and Optimization of Terpene-Enriched Vesicles (Terpesomes) for Effective Ocular Delivery of Fenticonazole Nitrate: In vitro Characterization and in vivo Assessment

Cited by 34 publications

References 41 publications

Tailoring Terpesomes and Leciplex for the Effective Ocular Conveyance of Moxifloxacin Hydrochloride (Comparative Assessment): In-vitro, Ex-vivo, and In-vivo Evaluation

Tailoring Terpesomes and Leciplex for the Effective Ocular Conveyance of Moxifloxacin Hydrochloride (Comparative Assessment): In-vitro, Ex-vivo, and In-vivo Evaluation

Investigation of the Potential of Nebivolol Hydrochloride-Loaded Chitosomal Systems for Tissue Regeneration: In Vitro Characterization and In Vivo Assessment

Carbon Nanotube Hybrid Materials: Efficient and Pertinent Platforms for Antifungal Drug Delivery

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