Development and identification of a wheat-Psathyrostachys huashanica addition line carrying HMW-GS, LMW-GS and gliadin genes

Zhao, Jixin; Ji, Wanquan; Wu, Jun; Chen, Xinhong; Cheng, Xueni; Wang, Jianwei; Pang, Yuhui; Liu, Shuhui; Yang, Qin

doi:10.1007/s10722-009-9477-4

Cited by 44 publications

(25 citation statements)

References 31 publications

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“…P. huashanica is characterized by a dwarfed stature, early maturity, with resistance to drought, salinity, wheat stripe rust and take-all fungus. It is therefore a potentially useful germplasm source for wheat improvement (Chen et al 1991;Jing et al 1999;Wang and Shang 2000;Zhao et al 2004Zhao et al , 2010Kang et al 2009). In order to transfer desirable traits from P. huashanica into wheat, our research team successfully hybridized common wheat and P. huashanica without using embryo rescue, and developed a new intergeneric amphiploid line (PHW-SA, 2n = 8x = 56, AABBD DNsNs) with resistance to stripe rust (Kang et al 2008(Kang et al , 2009.…”

Section: Introductionmentioning

confidence: 99%

Production and cytogenetics of trigeneric hybrid involving Triticum, Psathyrostachys and Secale

Kang

Zhong

Xie

et al. 2011

Genet Resour Crop Evol

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Trigeneric hybrids may help establish evolutionary relationships among different genomes present in the same cellular-genetic background, and also offers the possibility to transfer different alien characters into cultivated wheat. In this study, a new trigeneric hybrid involving species from the Triticum, Psathyrostachys and Secale was synthesized by crossing wheat-P. huashanica amphiploid (PHW-SA) with wheat-S. cereale amphiploid (Zhongsi 828). The crossability of F 1 hybrid was high with 35.13%, and the fertility was 41.95%. The morphological characteristics of F 1 plants resembled the parent Zhongsi 828. The trigeneric hybrids pollen mother cells (PMCs) regularly revealed averagely 19.88 univalents, 9.63 ring bivalents, 3.97 rod bivalents, 0.60 trivalents and 0.03 tetravalents per cell. Multivalents consisted of trivalents and tetravalents can be observed in 52.7% of cells. A variation of abnormal lagging chromosome, micronuclei and chromosome bridge were formed at anaphase I and telophase II. The mean chromosomes number of F 2 progenies was 2n = 46.13, and the distribution range was 42-53. GISH results revealed that most F 2 plants had 6-12 S. cereale chromosomes, and only 0-2 P. huashanica chromosomes were detected. The results indicated that S. cereale chromosomes can be preferentially transmitted in the F 2 progenies of trigeneric hybrid than P. huashanica chromosomes. A survey of disease resistances revealed that the stripe rust resistance from the PHW-SA were completely expressed in the F 1 and some F 2 plants. The trigeneric hybrid could be a useful bridge for the transference of P. huashanica and S. cereale chromatins to common wheat.

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Section: Introductionmentioning

confidence: 99%

Production and cytogenetics of trigeneric hybrid involving Triticum, Psathyrostachys and Secale

Kang

Zhong

Xie

et al. 2011

Genet Resour Crop Evol

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“…Several P. huashanica genes have already been introgressed successfully into the wheat (Triticum aestivum L.) genome (Zhao et al, 2010;Du et al, 2013a,b). In the 1990s, we generated (Chen et al, 1991(Chen et al, , 1996 the F 1 hybrid H881 (2n = 28, ABDNs) and a derived heptaploid H8911 (2n = 49, AABBDDNs) of common wheat cv.…”

Section: Introductionmentioning

confidence: 99%

A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines

Wang

Lü

et al. 2013

Span J Agric Res

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The genome of Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) contains many desirable characteristics, such as resistance to biotic and abiotic stresses, which make it suitable for wheat improvement (Kang et al., 2009). Several P. huashanica genes have already been introgressed successfully into the wheat (Triticum aestivum L.) genome (Zhao et al., 2010; Du et al., 2013a,b). In the 1990s, we generated (Chen et al., 1991(Chen et al., , 1996 the F 1 hybrid H881 (2n = 28, ABDNs) and a derived heptaploid H8911 (2n = 49, AABBDDNs) of common wheat cv. 7182 and P. huashanica (GenBank Acc. No. 0503383) via embryo culture, two backcrosses, and selfing. As a result, we generated a large and complex range of wheat-P. huashanica offspring that carried different chromosome number or structure variants, i.e., chromosome additions, deletions, and rearrangements. However, a major problem was the selection of recombinant plants derived from the large interbred population. After several years of screening and identification, a complete set of wheat-P. huashanica disomic addition lines (1Ns -7Ns, 2n = 44 = 22II) was developed. This was a repetitive and difficult work. Although cytogenetic techniques proved to be very useful tools for alien identification, they had limitations because of the small population number, the complexity of the experimental techniques, and envi- AbstractPsathyrostachys huashanica Keng is an endangered species that is endemic to China, which provides an important gene pool for wheat improvement. We developed a quick and reliable PCR-based diagnostic assay to accurately and efficiently detect P. huashanica DNA sequences from introgression lines, which was based on a species-specific marker derived from genomic DNA. The 900-bp PCR-amplified band used as a P. huashanica-specific RAPD marker was tested with 21 different plant species and was converted into a sequence-characterized amplified region (SCAR) marker by cloning and sequencing the selected fragments (pHs11). This SCAR marker, which was designated as RHS-23, could clearly distinguish the presence of P. huashanica DNA repetitive sequences in wheat-P. huashanica derivative lines. The specificity of the marker was validated using 21 different plant species and a complete set of wheat-P. huashanica disomic addition lines (1Ns -7Ns, 2n = 44 = 22II). This specific sequence targeted the Ns genome of P. huashanica and it was present in all the seven P. huashanica chromosomes. Therefore, this SCAR marker is specific for P. huashanica chromosomes and may be used in the identification of alien repetitive sequences in large gene pools. This diagnostic PCR assay for screening the target genetic material may play a key role in marker-assisted selective breeding programs.Additional key words: addition lines; marker-assisted selection; repetitive sequences; RAPD; SCAR.* Corresponding author: cxh2089@126.com; wj2105@163.com Received: 24-03-13. Accepted: 15-10-13.This work has one Supplementary Figure that does not appear in the printed article but that accompan...

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“…It is characterized by high resistance to cold, salinity, drought, and barren conditions, and it also exhibits a dwarfed stature, early maturity, and resistance to scab, stripe rust, take-all, and powdery mildew (Zhao et al, 2010;Wang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A novel SCAR marker for detecting Psathyrostachys huashanica Keng chromatin introduced in wheat

Wang²,

Wang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. In this study, we cloned and sequenced a 938-base pair polymorphic band, pHs27, in the tightly linked random amplified polymorphic DNA marker OPU10 and converted it into a sequencecharacterized amplified region (SCAR) marker referred to as RHS141, which was specific for the Ns genome of Psathyrostachys huashanica. A GenBank basic local alignment search tool search showed that the sequence of pHs27 had no primary sequence homology with known sequences, and Southern blotting confirmed this result. This SCAR marker was used to detect Ns genome chromatin in wheat, and it was successfully amplified in P. huashanica itself, a complete set of wheat-P. huashanica disomic addition lines (1Ns-7Ns), and undetermined homoeologous group addition lines. This SCAR marker will be a powerful tool for the marker-assisted selection of P. huashanica chromosome(s) in a wheat background, and it should also allow wheat breeders to screen for the excellent traits found in P. huashanica chromatin.

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Development and identification of a wheat-Psathyrostachys huashanica addition line carrying HMW-GS, LMW-GS and gliadin genes

Abstract: Psathyrostachys huashanica Keng, in the family Poaceae, subtribe Triticeae is an endangered and endemic species of China.

Cited by 44 publications

References 31 publications

Production and cytogenetics of trigeneric hybrid involving Triticum, Psathyrostachys and Secale

Production and cytogenetics of trigeneric hybrid involving Triticum, Psathyrostachys and Secale

A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines

A novel SCAR marker for detecting Psathyrostachys huashanica Keng chromatin introduced in wheat

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