Development and evaluation of silk fibroin-based nerve grafts used for peripheral nerve regeneration

Yang, Yumin; Ding, Fei; Wu, Jian; Hu, Wen; Liu, Wei; Liu, Jie; Gu, Xiaosong

doi:10.1016/j.biomaterials.2007.09.001

Cited by 289 publications

(191 citation statements)

References 42 publications

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“…Similar findings were also observed in a previous study, though these results were limited by extensive swelling and collapse of the biomaterial poly(lactic co-glycolic acid) (PLGA) in the study. CMAP examinations offer an important index for the conduction function of peripheral nerve [2,[28][29][30][31]. In this study, we observed the highest CMAP amplitude of autograft repair in both plantar foot muscles and dorsal muscles.…”

Section: Discussionsupporting

confidence: 59%

“…Previous studies have reported on reinnervation after single-lumen nerve tube repair using various methods [2,7,8,[32][33][34]. The main goal of this research was to investigate whether multichannel nerve conduits present a permissive microenvironment for axonal growth.…”

Section: Discussionmentioning

confidence: 99%

“…Though an autologous nerve graft is the gold standard of nerve graft repair, it has several disadvantages, including the need for an extra incision, loss of donor nerve function, mismatch in size between the donor nerve and the injured nerve, and a limited availability of donor nerve. A diverse array of synthetic and natural materials has been studied in peripheral nerve repair, but these studies have shown limited success [1][2][3][4][5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit

et al. 2010

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Single channel conduits are used clinically in nerve repair as an alternative to the autologous nerve graft.Axons regenerating across single channel tubes, however, may disperse resulting in inappropriate target reinnervation. This dispersion may be limited by multichannel nerve conduits as they resemble the structure of nerve multiple basal lamina tubes. In this study, we investigated the influence of channel number on the axonal regeneration using a series of 1-, 2-, 4-, and 7-channel collagen conduits and commercial (NeuraGen ® ) single channel conduits. Nerve conduits were implanted in rats with a 1cm gap of sciatic nerve.After four months, quantitative results of regeneration were evaluated with nerve morphometry and the accuracy of regeneration was assessed using retrograde tracing: two tracers being applied simultaneously to tibial and peroneal nerves to determine the percentage of motor neurons with double projections. Recovery of function was investigated with compound muscle action potential recordings and ankle motion analysis. We showed that the fabricated 1-channel and 4-channel conduits are superior to other types of conduits in axonal regeneration. Simultaneous tracing showed a significantly lower percentage of motor neurons with double projections after 2-and 4-channel compared with 1-channel conduit repair. This study shows the potential influence of multichannel guidance on limiting dispersion without decreasing quantitative results of regeneration.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit

et al. 2010

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“…To normalize the data, values obtained from the operating hind limb were expressed as percentage of values of the contralateral limb for each animal. Latency period and compound action potential amplitude (CMAP) were then recorded (Yang et al 2007). …”

Section: Electrophysiological Analysismentioning

confidence: 99%

KLF7-transfected Schwann cell graft transplantation promotes sciatic nerve regeneration

Wang

Hua

et al. 2017

Neuroscience

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Abstract:Our former study demonstrated that Krüppel-like Factor 7 (KLF7) is a transcription factor that stimulates axonal regeneration after peripheral nerve injury. Currently, we used a gene therapy approach to overexpress KLF7 in Schwann Cells (SCs) and assessed whether KLF7-transfected SCs graft could promote sciatic nerve regeneration. SCs were transfected by AAV2-KLF7 in vitro. Mice was allografted by an acellular nerve (ANA) with either an injection of DMEM (ANA group), SCs (ANA+SCs group) or AAV2-KLF7-transfected SCs (ANA+KLF7-SCs group) to assess repair of a sciatic nerve gap. The results indicate that KLF7 overexpression promoted the proliferation of both transfected SCs and native SCs. The neurite length of the DRG explants was enhanced.Several beneficial effects were detected in the ANA+KLF7-SCs group including an increase in the compound action potential amplitude , sciatic function index score, enhanced expression of PKH26-labeling transplant SCs, peripheral myelin protein 0 , neurofilaments , S-100, and myelinated regeneration nerve.Additionally, HRP-labeled motoneurons in the spinal cord, CTB-labeled sensory neurons in the DRG, motor endplate density and the weight ratios of target muscles were increased by the treatment while thermal hyperalgesia was diminished. Finally, expression of KLF7, NGF, GAP43, TrkA and TrkB were enhanced in the grafted SCs, which may indicate that several signal pathways may be involved in conferring the beneficial effects from KLF7 overexpression. We concluded that KLF7-overexpressing SCs promoted axonal regeneration of the peripheral nerve and enhanced myelination, which collectively proved KLF-SCs as a novel therapeutic strategy for injured nerves.

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“…Several studies have demonstrated the potential of N-fibroin in the regeneration of peripheral nerve injuries [124][125][126][127][128]. Silk fibroin-based nerve guidance conduit with oriented filaments was produced by Yang and colleagues [129] and originated successful results in rat sciatic model. Researchers have also shown that silk-based conduits [130] and silk nanofibres [131] enhance cell-material interactions like cell adhesion, proliferation and differentiation.…”

Section: N-fibroinmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Human adult peripheral nerve injuries are a high incidence clinical problem that greatly affects patients' quality of life. Although peripheral nervous system has intrinsic regenerative capacity, this occurs in an incomplete or poorly functional manner. When a nerve fiber loses its continuity with consequent damage of the basal lamina tubes, axon spontaneous regeneration is disorganized and mismatched. These phenomena translate in an inadequate nerve functional recovery and consequent musculoskeletal incapacity. Nerve grafts still remain the gold standard in peripheral injuries treatment. However, this approach contains its disadvantages such as the necessity of primary surgery to harvest the autografts, loss of a functional nerve, donor site morbidity and longer surgery procedures. Therefore, biomaterials and tissue engineering can provide efficient resources and alternatives to nerve injury repair not only by the development of biocompatible structures but also, introducing neurotrophic factors and cellular systems to stimulate optimum clinical outcome. In this chapter, a comprehensive state-of-the art picture of tissue-engineered nerve grafts scaffolds, their application in nerve regeneration along with latest advances in peripheral nerve repair and future perspectives will be discussed, including our own large experience in this field of knowledge.

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Development and evaluation of silk fibroin-based nerve grafts used for peripheral nerve regeneration

Cited by 289 publications

References 42 publications

Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit

Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit

KLF7-transfected Schwann cell graft transplantation promotes sciatic nerve regeneration

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

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