Development and Evaluation of Low-Carb Cakes Produced from Green Bocaiuva Pulp Enriched with <i>Pleurotus Ostreatus</i>

Giunco, Aline Janaina; Paz, Marcelo Fossa da; Fonseca, Gustavo Graciano

doi:10.1080/15428052.2021.1929637

Cited by 1 publication

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“… 32 The use of vitamin C and quercetin combination was recently reported for prophylaxis and management of COVID-19 patients in addition to remdesivir or convalescent plasma. 38 During infection, vitamin C is concentrated within macrophages, is essential for neutrophil killing, is responsible for T cell maturation, in addition to promoting phagocytosis and apoptosis of spent neutrophils. 39 …”

Section: Discussionmentioning

confidence: 99%

In vitro Anti SARS-CoV-2 Activity and Docking Analysis of Pleurotus ostreatus, Lentinula edodes and Agaricus bisporus Edible Mushrooms

Elhusseiny¹,

El-Mahdy²,

Elleboudy³

et al. 2022

IDR

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Background Fungi are rich source of biologically active metabolites aimed for the improvement of human health through the prevention of various diseases, including infections and inflammatory disorders. Aim We aimed to in vitro examine the anti-SARS CoV-2 activity of the aqueous extract of each Pleurotus (P .) ostreatus , Lentinula ( L .) edodes and Agaricus ( A .) bisporus edible mushroom followed by docking analysis of certain metabolites against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-main protease (protease M pro ). Methods Antiviral and cytotoxic effects were tested on hCoV-19/Egypt/NRC-3/2020/Vero-E6 cells and analyzed via (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide Assay (MTT) assay. Ligand-protein and protein-protein docking studies were performed to explore the interaction of different mushroom extracts at the binding site of protease M pro . Molecular dynamics (MD) simulations were performed on the most promising ligand-target complexes to investigate their dynamic properties and confirm docking results. Results Substantial antiviral activities with an IC 50 of 39.19, 26.17, and 10.3.3 µg/mL and a selectivity index (SI) of 4.34, 3.44, and 1.5 for P. ostreatus , L. edodes and A. bisporus , were observed, respectively. Docking analysis revealed that, catechin from three mushroom isolates, chlorogenic acid from A. bisporus , kamperferol of P. ostreatus and quercetin from L. edodes , with a C-DOCKER interaction energy in the range of 22.8–37.61 (Kcal/mol) with protease compared to boceprevir ligand of 41.6 (Kcal/mol). Docking of superoxide dismutase, catalase from the three mushrooms, tyrosinase from A. bisporus showed ligand contact surface area with the protein as 252.74 Å 2 while receptor contact surface area was 267.23 Å 2 . Conclusion P. ostreatus , L. edodes and A. bisporus have potential and remarkable in vitro antiviral activities against SARS-CoV-2. Quercetin from L. edode s, Kaempferol from P. ostreatus , chlorogenic acid and ascorbic acid, catechin, superoxide dismutase and catalase of the three mushrooms extracts were effectively bounded to M pro of SARS-CoV-2 as conferred by docking analysis.

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Section: Discussionmentioning

confidence: 99%