Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of
            <i>Bordetella pertussis</i>
            Infection

Kamachi, Kazunari; Toyoizumi-Ajisaka, Hiromi; Toda, Kohei; Soeung, Sann Chan; Svay, Sarath; Nareth, Ya; Horiuchi, Yoshinobu; Kojima, Kazunobu; Takahashi, Motohide; Arakawa, Yoshichika

doi:10.1128/jcm.44.5.1899-1902.2006

Cited by 80 publications

(77 citation statements)

References 25 publications

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“…LAMP is a nucleic acid amplification method that was developed in Japan as a simple, easy, and fast assay used to detect B. pertussis DNA (3,5). In our study, all 57 cases of microbiologically confirmed B. pertussis infections were LAMP-positive, while only 60z were culture-positive.…”

Section: Discussionmentioning

confidence: 73%

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Clinical Manifestations of Children with Microbiologically Confirmed Pertussis Infection and Antimicrobial Susceptibility of Isolated Strains in a Regional Hospital in Japan, 2008^|^ndash;2012

et al. 2014

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SUMMARY:We conducted a retrospective study in 57 children (median age, 3.5 years; range, 1 month-14.5 years) with microbiologically confirmed pertussis infection over a recent 4-year period in a regional hospital in Japan. We obtained nasal swabs from all patients for Bordetella pertussis isolation as well as performed B. pertussis DNA detection using loop-mediated isothermal amplification (LAMP). Of the 57 cases, 34 (60z) were culture-positive and 57 (100z) were LAMP-positive. The frequency of each symptom was as follows: typical paroxysmal cough for over 14 days, 96z (55/57); paroxysms, 86z (49/57); posttussive vomiting, 33z (19/57); inspiratory whoop, 25z (14/57); and apnea, 12z (7/57). Hospitalization was required in 14 cases (25z), 93z (13/14) of which were aged º1 year. The proportion of patients previously immunized against diphtheria-tetanus-acellular pertussis vaccine (DTaP) was 19z (4/21) in children aged º 1 year and 92z (11/12) in children aged AE 10 years. Minimum inhibitory concentrations for 6 antimicrobials (erythromycin, clarithromycin, azithromycin, minocycline, amoxicillin, and sulfamethoxazole/trimethoprim) were measured for 30 isolated strains, and all strains were susceptible to all aforementioned antimicrobials. Thus, an additional pertussis vaccination in older children is necessary, and the current macrolides-based treatment strategy is considered reasonable.

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Section: Discussionmentioning

confidence: 73%

“…Primers for the LAMP assay were designed on the basis of PT promoter region of the B. pertussis strain Tohama (genome position, 159549-159755; GenBank accession number: BX640422). This assay demonstrated a high agreement level with conventional polymerase chain reaction (PCR) results (3).…”

Section: Methodsmentioning

confidence: 88%

Clinical Manifestations of Children with Microbiologically Confirmed Pertussis Infection and Antimicrobial Susceptibility of Isolated Strains in a Regional Hospital in Japan, 2008^|^ndash;2012

et al. 2014

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“…Compared with the culture method, nucleic acid amplification tests such as the IS481-based real-time polymerase chain reaction (PCR) and B. pertussis-specific loop-mediated isothermal amplification (LAMP) are highly sensitive for the detection of B. pertussis DNA (17)(18)(19). Therefore, these nucleic acid amplification tests have been recommended for the accurate diagnosis of pertussis (20).…”

Section: Introductionmentioning

confidence: 99%

Concentrations of immunoglobulin G antibodies against pertussis toxin does not decrease over a long period of time in Japan

Sakakibara

Ohtani²,

Jinta

et al. 2015

10.1 Respiratory Infections

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Objective Adult patients with pertussis rarely show typical symptoms, such as paroxysmal coughing, inspiratory "whoop", or post-tussive vomiting. While a culture is regarded as the gold standard for diagnosis, the sensitivity is very low. Therefore, the diagnosis of pertussis in adults in clinical practice is mostly based on single-sample serology using an enzyme-linked immunosorbent assay (ELISA) with the pertussis toxin antigen. Various cut-off values for the anti-pertussis toxin IgG (PT-IgG) have been proposed. It has been reported that concentrations of PT-IgG fall below the defined cut-off about 4.5 months after infection on average, and within 1 year in most patients. We investigated the distribution and time course of the PT-IgG levels. Methods The data were collected from the medical records. Patients The study retrospectively identified subjects who had visited Ikebukuro Otani Clinic, which is a specialized clinic for patients with cough. We retrospectively reviewed 406 patients with PT-IgG measurements to investigate the age distribution of PT-IgG levels. The changes in PT-IgG levels over time were assessed in the 205 patients who had more than one PT-IgG measurement. Results PT-IgG levels were ! 100 EU/mL in more than 15% of subjects. The PT-IgG levels of a few subjects had diminished over a long period of time. Conclusion A PT-IgG level greater than the defined cut-off value simply indicates past infection or immunization in most subjects. As such, a single measurement of PT-IgG using the cut-off values might lead to overdiagnosis of pertussis. Further data collection and analysis are required.

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“…LAMP is a very simple and fast procedure that does not require specific equipment. It has a high sensitivity and specificity because the LAMP primers target eight regions in approximately 200 nucleotides (4). Results are obtained within 1 hour, inclusive DNA extraction and genome amplification steps.…”

mentioning

confidence: 99%

“…In the present study, bacterial isolation and loopmediated isothermal amplification (LAMP) for the detection of B. pertussis genomic DNA were tested, using a primer set reported by Kamachi et al (4). The test subjects consisted of 43 patients aged ＜15 years who were suspected to have pertussis and were treated at the Saiseikai Central Hospital, Tokyo in 2010 and 2011.…”

mentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP) Aids the Clinical Diagnosis of Pertussis

et al. 2015

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Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of Bordetella pertussis Infection

Cited by 80 publications

References 25 publications

Clinical Manifestations of Children with Microbiologically Confirmed Pertussis Infection and Antimicrobial Susceptibility of Isolated Strains in a Regional Hospital in Japan, 2008^|^ndash;2012

Clinical Manifestations of Children with Microbiologically Confirmed Pertussis Infection and Antimicrobial Susceptibility of Isolated Strains in a Regional Hospital in Japan, 2008^|^ndash;2012

Concentrations of immunoglobulin G antibodies against pertussis toxin does not decrease over a long period of time in Japan

Loop-Mediated Isothermal Amplification (LAMP) Aids the Clinical Diagnosis of Pertussis

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