Development and evaluation of a pan‐dermatophyte polymerase chain reaction with species‐level identification using sloppy molecular beacon probes

Walser, Michael; Bosshard, Philipp P.

doi:10.1111/bjd.17512

Cited by 11 publications

(9 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, supporters of modern contact methods can receive both the identification result and the prescription electronically, without the patient visiting the hospital or doctor's office again (Walser and Bosshard 2019). The result of molecular identification can be obtained on the day the sample reaches the laboratory or the next day, whereas fungal culture can take up to 1-3 weeks (Brillowska-Dabrowska et al 2010;Gnat et al 2017;Verrier and Monod 2017;Arastehfar et al 2019b;Walser and Bosshard 2019). The shortest time of 3 h from delivery of the material to obtaining the result is given in the method proposed by Miyajma et al (2013).…”

Section: Development Of a Diagnostic Pathmentioning

confidence: 99%

Major challenges and perspectives in the diagnostics and treatment of dermatophyte infections

2020

View full text Add to dashboard Cite

Dermatophytes are the aetiological factors of a majority of superficial fungal infections. What distinguishes them from other pathogenic filamentous fungi is their unique ability to degrade keratin. The remarkable ability of this group of fungi to survive in different ecosystems results from their morphological and ecological diversity as well as high adaptability to changing environmental conditions. Paradoxically, despite the progress in medicine, the prevalence of dermatophyte infections is increasing from year to year. At the beginning of the third millennium, practical diagnostic and therapeutic options are still very limited. This review focuses on understanding the major problems in this aspect of dermatophyte infections and indicates future strategies and perspectives for novel approaches to identification and drugs for elimination of dermatophytes. Particular importance is placed on development of a strategy for a diagnostic pathway and implementation of rapid and reliable diagnostics methods designed by international teams. Furthermore, among compounds that currently arouse great interest, representatives of terpenoids, alkaloids, saponins, flavonoids and essential oils deserve attention. Many of these compounds are undergoing clinical trials as potential antifungal agents, and future research should focus on attempts at determination of the applicability of tested substances. Finally, the advantages and disadvantages in implementation of new diagnostic paths and medicinal substances for routine use are indicated. Journal of Applied Microbiology

show abstract

Section: Development Of a Diagnostic Pathmentioning

confidence: 99%

Major challenges and perspectives in the diagnostics and treatment of dermatophyte infections

2020

View full text Add to dashboard Cite

show abstract

“…The culture positivity rate even in smear-positive specimens (resembling dermatophytes) is not more than 65-75%. 6 Flat, white, fastgrowing and expanding colonies with hyphae bundles in the periphery and slight yellowish-to-beige pigmentation in the center are typical for T. mentagrophytes of the Indian genotype [Figures 1a and b]. The reverse side of the colonies shows yellowish-to-beige and brown pigmentation [Figures 1c and d].…”

Section: Cultural Features Of Trichophyton Mentagrophytesmentioning

confidence: 99%

The unprecedented epidemic-like scenario of dermatophytosis in India: II. Diagnostic methods and taxonomical aspects

Verma¹,

Panda²,

Nenoff³

et al. 2021

IJDVL

View full text Add to dashboard Cite

Trichophyton (T.)mentagrophytes now accounts for an overwhelming majority of clinical cases in India, a new “Indian genotype” (T. mentagrophytes ITS genotype VIII) having been isolated from skin samples obtained from cases across a wide geographical distribution in this country. The conventional diagnostic methods, like fungal culture, are, however, inadequate for diagnosing this agent. Thus, molecular methods of diagnosis are necessary for proper characterization of the causative agent. The shift in the predominant agent of dermatophytosis from T. rubrum to T. mentagrophytes, within a relatively short span of time, is without historic parallel. The apparent ease of transmission of a zoophilic fungus among human hosts can also be explained by means of mycological phenomena, like anthropization.

show abstract

“…Sensitivity and specificity of PCR for detection of dermatophytes in clinical samples were estimated to be 96.9% and 90 4% for culture 46.7% and 98.7%, and for microscopy 91.4% and 84.0%, respectively. The detection of non-dermatophytes molds by PCR and culture did not correlate [33]. Table 4 summarises various studies comparing commercial kits versus in-house real time PCR techniques.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Multiplex Real-Time PCR DermaGenius® Assay for the Detection of Dermatophytes in Hair Samples from Senegal

Ndiaye

Sacheli

Diongue

et al. 2021

JoF

View full text Add to dashboard Cite

For the successful treatment of dermatophytoses, especially tinea capitis, there is a need for accurate and rapid diagnostic methods. A lot of recent literature has focused on the detection of dermatophytes directly on sample material such as nails, hair and skin scrapings. Molecular tools offer the ability to rapidly diagnose dermatophytosis within 48 h. This study aimed to compare the results of a commercial real-time PCR (real-time PCR) assay DermaGenius®(DG) 2.0 complete multiplex kit with those of conventional diagnostic methods (direct microscopy and culture). A total of 129 hair samples were collected in Dakar (Senegal) from patients suspected of dermatophytosis. DG was applied for the molecular detection of Candida albicans, Trichophyton rubrum/soudanense, T. interdigitale, T. tonsurans, T. mentagrophytes, T. violaceum, Microsporum canis, M. audouinii, Epidermophyton floccosum, T. benhamiae and T. verrucosum. Dermatophytes species and C. albicans were differentiated by melting curve analysis. The sensitivity and specificity of the PCR assay were 89.3% and 75.3%, respectively. DG PCR was significantly more sensitive than culture (p < 0.001). DG PCR is fast and robust to contamination. In this paper, the main questions discussed were the replacement of culture by a broad-spectrum fungal real-time PCR and the implementation of DG PCR into a routine laboratory in Senegal.

show abstract

Development and evaluation of a pan‐dermatophyte polymerase chain reaction with species‐level identification using sloppy molecular beacon probes

Cited by 11 publications

References 29 publications

Major challenges and perspectives in the diagnostics and treatment of dermatophyte infections

Major challenges and perspectives in the diagnostics and treatment of dermatophyte infections

The unprecedented epidemic-like scenario of dermatophytosis in India: II. Diagnostic methods and taxonomical aspects

Evaluation of the Multiplex Real-Time PCR DermaGenius® Assay for the Detection of Dermatophytes in Hair Samples from Senegal

Contact Info

Product

Resources

About