Development and characterization of nine new microsatellite markers in Taraxacum (Asteraceae)

Abstract

This study aims at developing and characterizing new microsatellite primer pairs in Taraxacum officinale auct. to produce polymorphic markers for genetical and evolutionary studies on apomixis in this sexual‐apomictic complex. A total of 24 diploid plants were tested for allelic polymorphism and heterozygosity. Out of nine loci three deviated significantly from the Hardy–Weinberg equilibrium expectations, probably due to the presence of null‐alleles. Successful cross‐species amplification was obtained for all markers in 29 Taraxacum microspecies from five sections.

Alien pollen grains interfere with the reproductive success of native congener

“…Therefore, leaf samples were genotyped and searched for speciescharacteristic alleles at each site using the eight microsatellite loci developed by Falque et al (1998) and Vašut et al (2004 ; Table 1). Species discrimination was achieved when the genotyped pollen grain had at least one species-characteristic allele.…”

Taraxacum pudicum, a new apomictic microspecies of T. section Erythrosperma (Asteraceae) from Central Europe

“…Based on these field observations, T. slovacum is very likely just a remarkable morphotype within the range of variability of the sexual species T. erythrospermum and is not related to T. pudicum. The first author published genetic data (Vašut et al 2004) for T. pudicum under the name "T. aff. slovacum Klášt. "…”

“…Flowcytometric seed screening (Matzk et al 2000) was used to test for reproductive mode. Microsatellites and AFLPs (Amplified Fragment Length Polymorphism) were used to test genetic diversity (Vašut et al 2004, Majeský et al 2012. Plants were cultivated by both authors for a period of more than 10 years (with gaps since 1998) to confirm morphological homogeneity among the populations as well as among the offspring.…”

“…Karyology:-2n = 3x = 24 (counted from the holotype locality); 2n ~ 3x, distinguished by flow-cytometry (Vašut et al 2004).…”

Epigenetic inheritance in apomictic dandelions : stress-induced and heritable modifications in DNA methylation and small RNA

“…The DNA was stored at -20°C in 50 µl TE until DNA was collected for all generations. For the selection of distinct lineages eight previously, for dandelion genotyping, established microsatellites were used (msta31, msta44B, msta58, msta78, msta61, msta67, msta72, msta143; Falque et al 1998;Vašut et al 2004) and the first 4 microsatellites were used for a second genotyping at the end of the transplantation experiment to confirm correct sample identification of experimental transplants (see Table 4.1S, supporting information).…”