Development and characterization of microsatellite loci from lynx (<i>Lynx canadensis</i>), and their use in other felids

Carmichael, Lindsey; Clark, William R.; Strobeck, Curtis

doi:10.1046/j.1365-294x.2000.105323.x

Cited by 34 publications

(25 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA was extracted using the DNeasy kit (Qiagen, Germany) following the manufacturer's instructions (final DNA-elution in 80 ll). One of each primer pair for six microsatellite loci (FCA08, FCA43, FCA77, FCA78;Menotti-Raymond et al 1999 and LC109 and LC110;Carmichael et al 2000) was 5 0 -labelled with fluorescent dye (6-FAM or HEX) and used for genotyping. Four additional loci (FCA35, FCA45, FCA90 and LCA120) could not be amplified in numerous samples and had to be excluded.…”

Section: Methodsmentioning

confidence: 99%

Genetic structure of the Eurasian lynx population in north-eastern Poland and the Baltic states

et al. 2009

View full text Add to dashboard Cite

We analyzed the genotypes of Eurasian lynx (Lynx lynx) from three populations in the westernmost part of the species main range. One population was situated at the distribution edge (NE Poland) and the two other (Latvia and Estonia) were located within the main, contiguous range of the species. The aim was to determine if the genetic composition varied among these populations and if there was evidence of isolation among them. Based on microsatellite allele frequencies, we found the allelic richness in Polish lynx to be lower than that in lynx from Latvia and Estonia. We also found significant differentiation among the lynx populations, with the NE Poland population forming a distinct genetic group relative to the two other populations (R ST = 0.15 and 0.22, P \ 0.0001). We suggest that genetic differentiation among lynx populations is the result of habitat insularisation that limits gene flow. This finding emphasizes the necessity to consider the lynx genetic differentiation in conservation planning of this species in Poland.

show abstract

Section: Methodsmentioning

confidence: 99%

Genetic structure of the Eurasian lynx population in north-eastern Poland and the Baltic states

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Each sample was genotyped at 13 known felid microsatellite loci (Menotti-Raymond et al 1999, 2005Carmichael et al 2000;Faircloth et al 2005). These data were used to assess common population genetic parameters (e.g., heterozygosity and inbreeding coefficients) and to evaluate subpopulation structure.…”

Section: Effects Of Roads On Bobcat Population Structurementioning

confidence: 99%

Bobcats (Lynx rufus) as a Model Organism to Investigate the Effects of Roads on Wide-Ranging Carnivores

Litvaitis

Reed

Carroll

et al. 2015

Environmental Management

View full text Add to dashboard Cite

We are using bobcats (Lynx rufus) as a model organism to examine how roads affect the abundance, distribution, and genetic structure of a wide-ranging carnivore. First, we compared the distribution of bobcat-vehicle collisions to road density and then estimated collision probabilities for specific landscapes using a moving window with road-specific traffic volume. Next, we obtained incidental observations of bobcats from the public, camera-trap detections, and locations of bobcats equipped with GPS collars to examine habitat selection. These data were used to generate a cost-surface map to investigate potential barrier effects of roads. Finally, we have begun an examination of genetic structure of bobcat populations in relation to major road networks. Distribution of vehicle-killed bobcats was correlated with road density, especially state and interstate highways. Collision models suggested that some regions may function as demographic sinks. Simulated movements in the context of the cost-surface map indicated that some major roads may be barriers. These patterns were supported by the genetic structure of bobcats. The sharpest divisions among genetically distinct demes occurred along natural barriers (mountains and large lakes) and in road-dense regions. In conclusion, our study has demonstrated the utility of using bobcats as a model organism to understand the variety of threats that roads pose to a wide-ranging species. Bobcats may also be useful as one of a group of focal species while developing approaches to maintain existing connectivity or mitigate the negative effects of roads.

show abstract

“…Initially, we genotyped the extracted DNA at 21 microsatellite loci; 6 characterized in lynx (Lc106, Lc109, Lc110, Lc111, Lc118, and Lc120; Carmichael et al 2000) and 15 in the domestic cat (Fca008, Fca031, Fca035, Fca043, Fca045, Fca077, Fca078, Fca090, Fca096, Fca149, Fca391, Fca441, Fca559, F41, and F115;Menotti-Raymond et al 1999). We used capillary electrophoresis on an Applied Biosystems (ABI) 3730 DNA Analyzer using GeneScan 500 ROX as a size standard.…”

Section: Sample Collection and Summary Statisticsmentioning

confidence: 99%

Dispersal promotes high gene flow among Canada lynx populations across mainland North America

et al. 2012

View full text Add to dashboard Cite

The amount and extent of dispersal can have a large effect on the evolutionary trajectory, dynamics and structure of populations. Thus, understanding patterns of genetic structure provide information about the needs and approaches for population management and species conservation. To date studies addressing the population structure of Canada lynx (Lynx canadensis) have been surprisingly equivocal, despite a large amount of research quantifying population cyclicity and synchrony and the species' species at-risk status in the contiguous United States and eastern provinces of Canada. Here we use 17 microsatellite loci to conduct a large-scale genetic structuring assessment for Canada lynx, including most of its geographic range from Alaska to Newfoundland. We found large differentiation between lynx populations on the island of Newfoundland and those on the mainland. Yet, contrary to previous studies we found little genetic differentiation (F ST , D est , R ST ) owing to the Rocky Mountains, but some evidence of a subtle gene flow restriction between Ontario and Manitoba as previously proposed to be the result of a climatic barrier. Bayesian clustering analysis, however, only suggested two genetic clusters, one consisting of lynx from Newfoundland, and the other consisting of lynx from the rest of the North American range. Because Canada lynx are harvested for fur across most of their range, our results are informative for effective management strategies (e.g., defining management units) aimed at ensuring long-term population connectivity and species persistence.

show abstract

Development and characterization of microsatellite loci from lynx (Lynx canadensis), and their use in other felids

Cited by 34 publications

References 5 publications

Genetic structure of the Eurasian lynx population in north-eastern Poland and the Baltic states

Genetic structure of the Eurasian lynx population in north-eastern Poland and the Baltic states

Bobcats (Lynx rufus) as a Model Organism to Investigate the Effects of Roads on Wide-Ranging Carnivores

Dispersal promotes high gene flow among Canada lynx populations across mainland North America

Contact Info

Product

Resources

About