Development and Application of Surface Plasmon Resonance-Based Biosensors for the Detection of Cell–Ligand Interactions

Quinn, John; O’Neill, Shane; Doyle, Aidan M.; McAtamney, Colm; Diamond, Dermot; MacCraith, Brian D.; O’Kennedy, Richard

doi:10.1006/abio.2000.4564

Cited by 144 publications

(92 citation statements)

References 13 publications

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“…Although the noise is an issue and relatively greater than in SPR, SPRi is utilized in many applications for detection of cells and bacteria [78,79]. In one approach, the SPRi sensor surface was modified with a hydrogelbased interaction layer.…”

Section: Importance Of Surface Morphology In Single Particle Optical mentioning

confidence: 99%

“…In one approach, the SPRi sensor surface was modified with a hydrogelbased interaction layer. When red blood cell-surface antibody interaction is investigated real time, it was found that the noise level was very high due to the non-specific binding and remaining total mass of cell debris on the surface [79]. As a result, only limited number of cell binding was detected.…”

Section: Importance Of Surface Morphology In Single Particle Optical mentioning

confidence: 99%

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Surface chemistry and morphology in single particle optical imaging

et al. 2017

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Biological nanoparticles such as viruses and exosomes are important biomarkers for a range of medical conditions, from infectious diseases to cancer. Biological sensors that detect whole viruses and exosomes with high specificity, yet without additional labeling, are promising because they reduce the complexity of sample preparation and may improve measurement quality by retaining information about nanoscale physical structure of the bio-nanoparticle (BNP). Towards this end, a variety of BNP biosensor technologies have been developed, several of which are capable of enumerating the precise number of detected viruses or exosomes and analyzing physical properties of each individual particle. Optical imaging techniques are promising candidates among broad range of label-free nanoparticle detectors. These imaging BNP sensors detect the binding of single nanoparticles on a flat surface functionalized with a specific capture molecule or an array of multiplexed capture probes. The functionalization step confers all molecular specificity for the sensor's target but can introduce an unforeseen problem; a rough and inhomogeneous surface coating can be a source of noise, as these sensors detect small local changes in optical refractive index. In this paper, we review several optical technologies for label-free BNP detectors with a focus on imaging systems. We compare the surface-imaging methods including dark-field, surface plasmon resonance imaging and interference reflectance imaging. We discuss the importance of ensuring consistently uniform and smooth surface coatings of capture molecules for these types of biosensors and finally summarize several methods that have been developed towards addressing this challenge.

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Section: Importance Of Surface Morphology In Single Particle Optical mentioning

confidence: 99%

Section: Importance Of Surface Morphology In Single Particle Optical mentioning

confidence: 99%

Surface chemistry and morphology in single particle optical imaging

et al. 2017

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“…Desde então, uma grande variedade de métodos para detecção de marcadores biológicos envolvendo processos cinéticos como adsorção-dessorção, interações proteína-ligante, interações antígeno-anticorpo, receptor ligante e proteína-DNA/RNA, vem sendo desenvolvidos. [46][47][48][49][50][51][52][53][54][55] As principais vantagens destes sensores plasmônicos se concentram em elevadas sensibilidades e medidas em tempo real sem a necessidade de marcação de biomoléculas.…”

Section: Aplicação De Nanopartículas Plasmônicas Em Sensores Biológicosunclassified

Ressonância de plasmon de superfície localizado e aplicação em biossensores e células solares

Santos¹,

Santos²,

Thesing³

et al. 2016

Quim. Nova

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LOCALIZED SURFACE PLASMON RESSONANCE APPLIED TO BIOSENSORS AND SOLAR CELLS. Within the last decades, the research on nanoparticles presenting localized surface plasmon resonance has increased constantly. In these materials, the interaction between electrons and incident light results in charge separation, enhancement of the electromagnetic field on the nanoparticles surface and in unique optical properties. Although many metals such as gold, silver, copper and aluminum present localized surface plasmon resonance within the visible range, gold and silver are the most commonly studied metals, due to the chemical inertia of gold and intense plasmon resonance from silver. In this review, we provide a description of the origin of localized surface plasmon resonance through the works developed by Mie, Maxwell and Maxwell-Garnett and a description of many examples of application of plasmonic nanoparticles on biosensors and solar cells, detailing the contribution of these plasmonic nanoparticles on the performance of these devices.Keywords: LSPR; Localized Surface Plasmon Resonance; biosensor; solar cell. INTRODUÇÃONos últimos 20 anos o interesse por nanopartículas metálicas (NPs-M) tem aumentado constantemente devido a sua ampla variedade de aplicações.1-8 Vários grupos de pesquisa têm estudado desde nanopartículas isoladas de cerca de 1 nm até nanopartículas de centenas de nanômetros, assim como seus aglomerados, buscando entender seu comportamento óptico e catalítico. Adicionalmente, a literatura descreve a constante busca por metodologias de síntese cada vez mais simples, de baixo custo e com alta reprodutibilidade. O comportamento óptico de nanopartículas metálicas tem fascinado a humanidade há vários séculos através de obras de arte, mesmo antes que a palavra nanotecnologia fosse utilizada.9 Um dos exemplos mais conhecidos é a Taça de Lycurgus (século 4 d.C), que tem em sua composição nanopartículas de ouro (NPAu).10 No entanto, foi somente em 1857 que as NPs-M receberam maior atenção devido aos estudos de Michael Faraday sobre o efeito da interação da luz em uma solução coloidal de NPAu obtida através da redução do cloreto de ouro pelo fósforo.11,12 Deste então, o interesse nestas NPs tem aumentado e novas metodologias de síntese e caracterização têm sido desenvolvidas, possibilitando grande controle de tamanho e formato.13-15 A pesquisa nesta área foi enriquecida pela compreensão do efeito da ressonância de plasmon de superfície localizado (LSPR), que explica o comportamento óptico destes materiais. Mie foi o primeiro a elucidar a origem das cores nestas estruturas e sua teoria foi posteriormente complementada com os modelos de Maxwell-Garnett que explicavam a interação de NPs-M com o meio. Estes estudos proporcionaram uma ampla visão quanto as possíveis aplicações das NPs-M, sendo atualmente empregada no desenvolvimento de novos materiais como células solares, biossensores e catalisadores. Nesta revisão, serão abordados os embasamentos teóricos envolvidos no efeito LSPR de NPs-M, destacando a aplicação destas n...

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“…Conventional SPR sensors have also been used to detect human RBCs based on blood group (Quinn et al, , 2000, and to extract binding kinetics of cells interacting with the functionalized surface (Li et al, 2008). The purpose of this paper is to provide deeper insight into human RBC detection based on blood group A antigen using LRSPP straight waveguides.…”

Section: Introductionmentioning

confidence: 99%

Biosensing using long-range surface plasmon-polariton waveguides

Krupin

Berini

2012

2012 International Conference on Optical MEMS and Nanophotonics

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Specific detection of biological matter is one of the key elements in a wide range of modern fields such as food industry, medicine, environmental and pharmaceutical industries. Generally, current common methods of detection (e.g. ELISA) involve molecular labelling, requirements for well-trained personnel and lengthy experimental procedures such as bacteria culture. All of the above issues result in high costs for biological analysis, and consequently, high costs for medical service, therapeutic drugs and various food products. Biosensors, on the other hand, can provide quick and cheap solutions to these problems.The field of optical biosensors is dominated by the method of surface plasmon resonance, which so far has attracted a lot of attention in the pharmaceutical industry.Investigation of long-range surface plasmon-polariton waveguides as an application for biosensing is still very novel, and most of it exists in the venue of theoretical discussions and modelling. The objective of this thesis is to demonstrate the capability of the novel optical biosensor based on plasmonic waveguides to selectively detect various biological entities in solutions.The experiments were conducted on photolithographically fabricated sensors consisting of straight gold waveguides embedded in low-refractive index fluoropolymer CYTOP and a microfluidic channel. As a proof-of-concept, a demonstration of basic sensing experiments such as detection of change in refractive index of bulk solution and non-specific adsorption of bovine serum albumin is provided. Further investigation of the sensor capabilities involved specific detection of human red blood cells and leukemia markers. Red blood cell detection was based on ABO blood grouping and included the estimation of limit of detection and signal-to-noise ratio for single cell detection. Finally, a clinically relevant problem of B-cell leukemia marker detection was targeted. The sensor demonstrated the ability to detect the relative abundance of similar proteins (immunoglobulin kappa and lambda) in a complex fluid (human serum). In addition, an experimental study on the optimization of the sensor for sensitivity was conducted.ii

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Development and Application of Surface Plasmon Resonance-Based Biosensors for the Detection of Cell–Ligand Interactions

Cited by 144 publications

References 13 publications

Surface chemistry and morphology in single particle optical imaging

Surface chemistry and morphology in single particle optical imaging

Ressonância de plasmon de superfície localizado e aplicação em biossensores e células solares

Biosensing using long-range surface plasmon-polariton waveguides

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