This study aimed to explain the cultural behavior of reading junior high school students in the internet era based on gender variables. The study was designed with a quantitative approach. The study population was high school students in four districts / cities in South Sulawesi and West Sulawesi, namely Makassar City, Soppeng Regency, Tana Toraja Regency, and Majene Regency. Data collection was done using a questionnaire. Analysis of research data was carried out through descriptive statistical analysis and inferential statistical analysis which included three stages, including; (1) data management, (2) statistical analysis, and (3) interpretation of results. Data were analyzed using IBM SPSS 23 and it was presented in the form of tables, diagrams, and verbal exposure. The results showed that there were significant differences in reading behavior of female students and male students based on (1) the type of reading material that is read, (2) the effort to obtain reading material, (3) the time used to read, (4) the values believed in reading habits, and (5) post-reading habits. The theoretical and practical implications are discussed.