Determining the Specific Status of Korean Collared Scops Owls

Abstract

The collared scops owl that occurs in Korea is a protected species but its exact specific status has been questioned. To resolve the species status, a molecular phylogenetic analysis was conducted using two fragments of mitochondrial DNA, cytochrome b (cyt b, 891 bp) and NADH dehydrogenase subunit 2 (ND2, 627 bp) genes. Phylogenetic trees of cyt b revealed that all Korean specimens formed a monophyletic group with Japanese scops owl Otus semitorques with very low sequence divergence (d= =0.008). We obtained a similar ND2 tree as well (d= =0.003); however, the genetic distance between Korean individuals and O. lempiji from GenBank (AJ004026-7, EU348987, and EU601036) was very high and sufficient enough to separate them as species (cyt b, d= =0.118; ND2, d= =0.113). We also found that Korean species showed high differentiation from O. bakkamoena (AJ004018-20 and EU601034; cyt b, d= =0.106; ND2, d= =0.113) and O. lettia (EU601109 and EU601033, cyt b, d= =0.110; ND2, d= =0.117) as well. Therefore, we suggest that the Korean collared scops owl should be designated as Otus semitorques.