Determining chemical rate coefficients using time-gated fluorescence correlation spectroscopy

Pulsed Interleaved Excitation

“…Different methods have been developed that utilize various combinations of the available information. Information over the duration of the fluorophore in the excitation state is used in time-gated FCS in connection with the absolute arrival time of the photon to remove background (14,15), to investigate the homogeneity of a sample, and to study the dynamics of heterogeneous distribution (16). Enderlein uses the same information in time-resolved FCS to resolve multiple fluorescence species with FCS (17).…”

Pulsed Interleaved Excitation

“…Different methods have been developed that utilize various combinations of the available information. Information over the duration of the fluorophore in the excitation state is used in time-gated FCS in connection with the absolute arrival time of the photon to remove background (14,15), to investigate the homogeneity of a sample, and to study the dynamics of heterogeneous distribution (16). Enderlein uses the same information in time-resolved FCS to resolve multiple fluorescence species with FCS (17).…”

Reducing Background Contributions in Fluorescence Fluctuation Time- Traces for Single-Molecule Measurements in Solution

“…Electronic early time-gating discriminates between which photons are incorporated in the analysis based on the time-delay between excitation of the fluorophore and emission of the fluorescence photon as first shown in ref [29]; the detector recorded the fluorescence bursts for a controlled time interval, e.g. in very large detection volumes of picoliters [29][30][31]. It is now performed entirely in software by using a time-correlated single-photon counting (TCSPC) card for data collection as shown in [32,33] and by others.…”

Pulsed Interleaved Excitation

“…Different methods have been developed that utilize various combinations of the available information. Information over the duration of the fluorophore in the excitation state is used in time-gated FCS in connection with the absolute arrival time of the photon to remove background (14,15), to investigate the homogeneity of a sample, and to study the dynamics of heterogeneous distribution (16). Enderlein uses the same information in time-resolved FCS to resolve multiple fluorescence species with FCS (17).…”

Reducing Background Contributions in Fluorescence Fluctuation Time- Traces for Single-Molecule Measurements in Solution

“…Electronic early time-gating discriminates between which photons are incorporated in the analysis based on the time-delay between excitation of the fluorophore and emission of the fluorescence photon as first shown in ref [29]; the detector recorded the fluorescence bursts for a controlled time interval, e.g. in very large detection volumes of picoliters [29][30][31]. It is now performed entirely in software by using a time-correlated single-photon counting (TCSPC) card for data collection as shown in [32,33] and by others.…”

Pulsed Interleaved Excitation

“…Different methods have been developed that utilize various combinations of the available information. Information over the duration of the fluorophore in the excitation state is used in time-gated FCS in connection with the absolute arrival time of the photon to remove background (14,15), to investigate the homogeneity of a sample, and to study the dynamics of heterogeneous distribution (16). Enderlein uses the same information in time-resolved FCS to resolve multiple fluorescence species with FCS (17).…”

Reducing Background Contributions in Fluorescence Fluctuation Time- Traces for Single-Molecule Measurements in Solution

“…Electronic early time-gating discriminates between which photons are incorporated in the analysis based on the time-delay between excitation of the fluorophore and emission of the fluorescence photon as first shown in ref [29]; the detector recorded the fluorescence bursts for a controlled time interval, e.g. in very large detection volumes of picoliters [29][30][31]. It is now performed entirely in software by using a time-correlated single-photon counting (TCSPC) card for data collection as shown in [32,33] and by others.…”