Determination of uric acid in human urine by ion chromatography with conductivity detector

Zhao, Fu Yong; Wang, Zong Hua; Wang, Hui; Zhao, Rui; Ding, Ming-Zhu

doi:10.1016/j.cclet.2010.10.008

Cited by 39 publications

(16 citation statements)

References 16 publications

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“…Among the hybrid methods for the determination of the concentration of USD markers the most wide spread are chromatographic [37][38][39][40] and electro phoretic [40][41][42][43][44][45][46] methods using a wide range of detec tors. Thus, for the determination of calcium and mag nesium ions in urine, ion chromatography with a piezoelectric detector was used [37].…”

Section: (5)mentioning

confidence: 99%

“…Thus, for the determination of calcium and mag nesium ions in urine, ion chromatography with a piezoelectric detector was used [37]. On a cation exchange column 15 cm in length (5 × 150 mm) using a mobile phase with an addition of tartaric acid and ethylendiamine (pH 4.0), the elution times of magne A similar method of the preparation of urine samples consisting of centrifugation and filtration stages was proposed for the determination of uric acid by ion chromatography with a conductometric detec tor in the isocratic mode [38]. The simultaneous determination of creatinine and uric acid was recommended to perform using HPLC with mass spectrometric [39] or ultraviolet detection [40].…”

Section: (5)mentioning

confidence: 99%

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Determination of markers of the urinary stone disease

Yaroshenko

Карцова

2015

J Anal Chem

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546Urinary stone disease (USD) is a metabolic disor der characterized by the formation of concrements (from Latin concrementum, aggregate, union), i.e., heavy masses in kidney and organs of urinary system [1]. The history of the study of USD markers is very long [2], and the interest to this problem keeps steadily growing (Fig. 1). The major publications dealing with various aspects of USD fall to the highly specialized medical journals, such as Journal of Urology, Urology, and European Urology Supplements. The variety of scientific editions on the given problem underlines its urgency (Fig. 2).Almost 40% of urological diseases belong to USD and a trend to its growth is observed [1, 2]. The mech anism of the stone formation process was discussed in [3]. For quite a long time USD can proceed asymp tomatically. The emergence of USD at the early stage can be revealed using biochemical urine analysis [3][4][5]. Urine is a solution of a complex composition because of the elevated salt background and the pres Abstract-The capabilities of physicochemical methods (chromatographic, electrophoretic, potentiometric, and voltammetric) and also field test methods for the determination of the main markers of the urinary stone disease have been considered. The effect of various factors on the processes of stone formation has been dis cussed.

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Section: (5)mentioning

confidence: 99%

Section: (5)mentioning

confidence: 99%

Determination of markers of the urinary stone disease

Yaroshenko

Карцова

2015

J Anal Chem

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“…[19] Uric acid Human urine 0.5 µg/L 0.10 to 20 mg/L Ion chromatography with conductivity detection. [20] Uric acid Dental calculi ----LC/MS [21] Uric acid Human urine 0.3 µM 1 to 100 µM Flow injection analysis with UV detection at 735 nm based on the reduction of Prussian Blue. [22] Honeychurch, K.C J Anal Bioanal Sep Tech |volume 2: issue 1…”

Section: Sample Detection Limit Linear Range Analytical Technique Refmentioning

confidence: 99%

The Determination of Uric Acid in Human Saliva by Liquid Chromatography with Electrochemical Detection

Honeychurch¹

2017

JABST

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A method employing high-performance liquid chromatography with electrochemical detection (LC-ED) has been successfully developed for the determination of uric acid in human salvia. The optimal chromatographic conditions were found to comprise a mobile phase containing 5 % methanol, 95 % 50 mM trichloroacetic acid, adjusted to pH 2.7, in conjunction with a Hypersil C 18 250 mm x 4.6 mm column at a flow rate of 0.6 mL min -1. Hydrodynamic voltammetric studies were undertaken to optimize the operating potentials required for electrochemical detection. It was found that an applied potential of +1.2 V was optimum for the determination of uric acid. The proposed method was evaluated by carrying out replicate uric acid determinations on spiked and unspiked human saliva samples (%CV = 8.8 %). The data suggest that the method holds promise for clinical applications.

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“…Therefore, new materials and methodology are urgently needed for separation, monitoring, determination, and diagnosis of uric acid from/in urine and human serum. Traditional techniques such as chemiluminescence , fluorescent biosensor , spectrophotometry , ion chromatography , and electrochemical methods have been developed for the determination of uric acid concentration in biological samples. However, these methods are usually laborious, expensive, time‐consuming, and/or complex to perform.…”

Section: Introductionmentioning

confidence: 99%

Photocontrolled extraction of uric acid from biological samples based on photoresponsive surface molecularly imprinted polymer microspheres

Gong

Liu

et al. 2017

J. Sep. Sci.

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We aim to develop novel photoresponsive surface molecularly imprinted polymer (SIMP) microspheres, an SiO -SIMP, for the photocontrolled extraction of uric acid from biological samples. The SiO -SMIP was prepared on silica microspheres by surface polymerization and characterized by using scanning electron microscopy, transmission electron microscopy, FTIR spectroscopy, thermogravimetric analysis, nitrogen adsorption-desorption analysis, and UV-visible spectroscopy. The SiO -SMIP microspheres showed a photocontrolled uptake and release of uric acid in NaH PO buffer upon alternate irradiation at 365 and 440 nm. The SiO -SMIP microspheres were able to photocontrollably extract uric acid from complicated biological samples for concentration analysis with no significant interference encountered and it exhibited very good recognition ability and fast binding kinetics toward uric acid.

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Determination of uric acid in human urine by ion chromatography with conductivity detector

Cited by 39 publications

References 16 publications

Determination of markers of the urinary stone disease

Determination of markers of the urinary stone disease

The Determination of Uric Acid in Human Saliva by Liquid Chromatography with Electrochemical Detection

Photocontrolled extraction of uric acid from biological samples based on photoresponsive surface molecularly imprinted polymer microspheres

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