1992
DOI: 10.1128/jvi.66.6.3784-3793.1992 View full text |Buy / Rent full text
|
|

Abstract: The poliovirus RNA polymerase error frequency was measured in vivo at eight sites in the poliovirus genome. The frequency at which specific G residues in poliovirion RNA changed to another base during one round of viral RNA replication was determined. Poliovirion RNA uniformly labeled with 32P1 was hybridized to a synthetic DNA oligonucleotide that was complementary to a sequence in the viral genome that contained a single internal G residue. The nonhybridized viral RNA was digested with RNase T1, and the prot… Show more

Help me understand this report

Search citation statements

Order By: Relevance
Select...
2
1
1
1
1
13
0

Year Published

2005
2005
2020
2020

Publication Types

Select...
3
2

Relationship

0
5

Authors

Journals

1
13
0
Order By: Relevance
“…While the stability of foreign gene expression is high for DNA viruses, that of RNA viruses is low, as RNA-dependent RNA polymerases lack a proofreading mechanism and have a high error rate (15,57,58). Furthermore, the genomic stability of positive-strand RNA viruses appears to be lower than that of negative-strand RNA viruses, probably because of the almost complete lack of recombination in the latter (15).…”
Section: Discussionmentioning
Create an account to read the remaining citation statements from this report. You will also get access to:
  • Search over 1b+ citation statments to see what is being said about any topic in the research literature
  • Advanced Search to find publications that support or contrast your research
  • Citation reports and visualizations to easily see what publications are saying about each other
  • Browser extension to see Smart Citations wherever you read research
  • Dashboards to evaluate and keep track of groups of publications
  • Alerts to stay on top of citations as they happen
  • Automated reference checks to make sure you are citing reliable research in your manuscripts
  • 14 day free preview of our premium features.

Trusted by researchers and organizations around the world

Over 100,000 students researchers, and industry experts at use scite

See what students are saying

rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…While the stability of foreign gene expression is high for DNA viruses, that of RNA viruses is low, as RNA-dependent RNA polymerases lack a proofreading mechanism and have a high error rate (15,57,58). Furthermore, the genomic stability of positive-strand RNA viruses appears to be lower than that of negative-strand RNA viruses, probably because of the almost complete lack of recombination in the latter (15).…”
Section: Discussionmentioning
“…Another essential factor in CNS invasion may be the involvement of EV quasispecies, or genotypic variants of virus populations, due to mutational errors accumulated during viral replication. These errors result from the low fidelity of the RNA-dependent RNA polymerase whereby at least one incorrect nucleotide is incorporated per genome duplication (Ward and Flanegan, 1992;Ward et al, 1988). Other contributing factors during viral replication may also increase viral diversity.…”
Section: Enterovirus Quasispeciesmentioning
“…One important factor in CNS invasion is the generation of genetic variants, otherwise known as quasispecies, which are produced during viral replication (Rhoades et al, 2011). Enteroviruses have a relatively low fidelity RNA-dependent RNA polymerase compared to eukaryotic hosts or DNA viruses (Ward and Flanegan, 1992) and high-fidelity polymerase has been correlated with decreased poliovirus fitness under selective pressure (Pfeiffer and Kirkegaard, 2005). The production of viral quasispecies was shown to be an important component for poliovirus neuroinvasion.…”
Section: Routes Of Enterovirus Neuroinvasionmentioning