Determination of the intracellular dissociation constant, KD, of the Fluo-3 � Ca2+ complex in mouse sperm for use in estimating intracellular Ca2+ concentrations

The development of signal transduction pathways during epididymal maturation is calcium dependent

“…440 5 & 6). The [Ca 2+ ] i of mouse caudal spermatozoa reported here is higher than that reported previously (Ref$$), but approximate that reported by others (535 ± 49 nM: Rockwell and Storey, 1999). In any case, the trends in the data support studies that have shown that the [Ca 2+ ] i of caput spermatozoa is 2 to 4-fold that of caudal cells (White and Aitken, 1989;Vijayaraghavan and Hoskins, 1990).…”

Enhanced Two-Photon Fluorescence and Fluorescence Imaging of Novel Probe for Calcium Ion by Self-Assembly with Conjugated Polymer

“…The value is 0.982 μmol/L, which is larger than those of some traditional Ca 2+ probes, such as Fluo-3 ( Kd = 0.39 μmol/L) [35]. This indicates that the BAPTAVP has a lower-affinity (higher Kd value) fluorescence probe for Ca 2+ .…”

Dendrites Contain a Spacing Pattern

“…Care was taken not to contact the culture monolayer with a pipette during media exchanges. We calculated the concentration of external calcium to be Ͻ1 nM during the second round of buffered zero-calcium DMEM [assuming K A EGTA ϭ 2.4 ϫ 10 7 M Ϫ1 (Rockwell and Storey, 1999)]. …”