Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.01.049 View full text
Hua-Chien Chen, Yu-Sun Chang, Shu-Jen Chen, Po-Ling Chang

Abstract: The methylation of the promoter region of DNA is an important regulatory mechanism for the downstream gene expression, and the extent of methylation has been linked to cancer formation. In this study, we report a simple method to screen for the degree of DNA methylation by combined bisulfite restriction analysis (COBRA) and capillary electrophoresis with laser-induced fluorescence (CE-LIF). After treating genomic DNA with sodium bisulfite, nested-PCR amplification and endonuclease (Taq I) digestion were perfor…

expand abstract