Determination of the heroin metabolite 6-acetylmorphine by high-performance liquid chromatography using automated pre-column derivatization and fluorescence detection

Chemical Reagents and Derivatization Procedures in Drug Analysis

“…(84) A detection limit of 0.2 pmol was possible for morphine after precolumn dansylation. (85) The heroin metabolite 6-acetylmorphine has been determined in urine by reversed-phase HPLC with fluorescence detection after automated precolumn oxidation with hexacyanoferrate(III) (86) at a reported detection limit of 1 ppb. Reserpine, an antihypertensive agent, was detected fluorimetrically after postcolumn reaction with nitrous acid and UV irradiation.…”

Chemical Reagents and Derivatization Procedures in Drug Analysis

“…(84) A detection limit of 0.2 pmol was possible for morphine after precolumn dansylation. (85) The heroin metabolite 6-acetylmorphine has been determined in urine by reversed-phase HPLC with fluorescence detection after automated precolumn oxidation with hexacyanoferrate(III) (86) at a reported detection limit of 1 ppb. Reserpine, an antihypertensive agent, was detected fluorimetrically after postcolumn reaction with nitrous acid and UV irradiation.…”

Water analysis

“…A fully automated online HPLC high-resolution gas chromatograph has been described along with a discussion on the instrumental aspects and application for the determination of heroin metabolites in urine (109). Detection of heroin consumption has been reported by determination of 6-monoacetylmorphine in urine by GC/MS (110,111) and by HPLC using automated precolumn derivatization and fluorescence detection (112). Morphine and codeine concentrations have been measured in urine samples, from individuals who had ingested poppy seed bagels, by GC/MS (113,114), by GC-NPD and GC/MS (115), and by capillary GC (116).…”

Chemical Reagents and Derivatization Procedures in Drug Analysis

“…(84) A detection limit of 0.2 pmol was possible for morphine after precolumn dansylation. (85) The heroin metabolite 6-acetylmorphine has been determined in urine by reversed-phase HPLC with fluorescence detection after automated precolumn oxidation with hexacyanoferrate(III) (86) at a reported detection limit of 1 ppb. Reserpine, an antihypertensive agent, was detected fluorimetrically after postcolumn reaction with nitrous acid and UV irradiation.…”

Water analysis

“…A fully automated online HPLC high-resolution gas chromatograph has been described along with a discussion on the instrumental aspects and application for the determination of heroin metabolites in urine (109). Detection of heroin consumption has been reported by determination of 6-monoacetylmorphine in urine by GC/MS (110,111) and by HPLC using automated precolumn derivatization and fluorescence detection (112). Morphine and codeine concentrations have been measured in urine samples, from individuals who had ingested poppy seed bagels, by GC/MS (113,114), by GC-NPD and GC/MS (115), and by capillary GC (116).…”

Chemical Reagents and Derivatization Procedures in Drug Analysis

“…(84) A detection limit of 0.2 pmol was possible for morphine after precolumn dansylation. (85) The heroin metabolite 6-acetylmorphine has been determined in urine by reversed-phase HPLC with fluorescence detection after automated precolumn oxidation with hexacyanoferrate(III) (86) at a reported detection limit of 1 ppb. Reserpine, an antihypertensive agent, was detected fluorimetrically after postcolumn reaction with nitrous acid and UV irradiation.…”

Water analysis

“…A fully automated online HPLC high-resolution gas chromatograph has been described along with a discussion on the instrumental aspects and application for the determination of heroin metabolites in urine (109). Detection of heroin consumption has been reported by determination of 6-monoacetylmorphine in urine by GC/MS (110,111) and by HPLC using automated precolumn derivatization and fluorescence detection (112). Morphine and codeine concentrations have been measured in urine samples, from individuals who had ingested poppy seed bagels, by GC/MS (113,114), by GC-NPD and GC/MS (115), and by capillary GC (116).…”