Determination of the Distribution of Selenium between Glutathione Peroxidase, Selenoprotein P, and Albumin in Plasma

Isolation of Selenoprotein-P and Determination of Se Concentration Incorporated in Proteins in Human and Mouse Plasma by Tandem Heparin Affinity and Size-exclusion Column HPLC-ICPMS

“…16 To solve these problems, researchers have developed a tandem approach combining a heparin AF column with either a blue sepharose AF column or an anion exchange column. [17][18][19][20] In their papers, validation was performed by comparing the sum of the Se concentrations in Sel-P, eGPx, and Se-Alb with the total Se concentration in serum or plasma. However, the adsorption efficiencies on the affinity columns were not obtained for each selenoprotein.…”

Relationship between plasma selenium concentrations and lower genital tract levels of HIV-1 RNA and interleukin type 1β

“…It is a limitation of this observational study that data on potentially important covariates such as plasma albumin, a selenium transport protein (Deagan et al, 1993) that has been associated with adverse HIV-1-related end points (Feldman et al, 2000(Feldman et al, , 2003, were not available. Secondly, analyses may have been confounded because the underlying immune impairments and inflammation may have depressed selenium levels (Drain et al, 2006).…”

Selenium (Se), Blood Glutathione Peroxidases and DNA Damage in Chronic Kidney Disease Patients on Hemodialysis and After Kidney Transplantation - The Effect of Se Supplementation

“…The most abundant selenoproteins in the blood components are selenoprotein P and GSH-Px (Ashton et al, 2009). Deagen et al (1993) have shown that the Se is incorporated, in the form of selenocysteine (Sec), to selenoprotein P (≈ 50% of plasma selenium) and GSH-Px (which accounts for 10-30% of plasma selenium) or bound in the form of selenomethionine to albumin (≈ 23%). Very similar results have been obtained for blood plasma in healthy subjects by Koyama et al (1999).…”