Determination of the activity of bacteriolytic enzymes and measurement of their sorption in the system of living cells of Lactobacillus plantarum

Matolygina, D. A.; Osipova, H. E.; Smirnov, Sergey; Belogurova, N. G.; Еремеев, Н. Л.; Тишков, В. И.; Levashov, Andrey V.; Левашов, П. А.

doi:10.3103/s002713141506005x

Cited by 10 publications

(10 citation statements)

References 15 publications

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“…Further increase in the glutamate concentrations does not significantly change this activity, which approaches a manner of threshold. A similar effect by glutamate on the activity of lysozyme and interleukin-2 can be explained based on the hypothesis that glutamate forms a complex with positively charged groups on the protein surface, preventing various types of nonproductive enzyme adsorption on cells, which may significantly change the apparent values of bacteriolytic activity parameters [ 11 , 12 ].…”

Section: Resultsmentioning

confidence: 99%

Bacteriolytic Activity Of Human Interleukin-2, Chicken Egg Lysozyme In The Presence Of Potential Effectors

et al. 2017

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The bacteriolytic activity of interleukin-2 and chicken egg lysozyme in the presence of various substances has been studied. Glycine and lysine do not affect the activity of interleukin-2 but increase that of lysozyme, showing a bell-shape concentration dependence peaking at 1.5 mM glycine and 18 mM lysine. Arginine and glutamate activate both interleukin-2 and lysozyme with a concentration dependence of the saturation type. Aromatic amino acids have almost no effect on the activity of both interleukin-2 and lysozyme. Aromatic amines, tryptamine, and tyramine activate interleukin-2 but inhibit lysozyme. Peptide antibiotics affect interleukin and lysozyme similarly and exhibit maximum activity in the micromolar range of antibiotics. Taurine has no effect on the activity of interleukin-2 and lysozyme. Mildronate showed no influence on lysozyme, but it activated interleukin-2 with the activity maximum at 3 mM. EDTA activates both interleukin-2 and lysozyme at concentrations above 0.15 mM.

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Section: Resultsmentioning

confidence: 99%

Bacteriolytic Activity Of Human Interleukin-2, Chicken Egg Lysozyme In The Presence Of Potential Effectors

et al. 2017

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“…Lyophilized Bifidobacterium bifidum (Microgen, Russia) was used for the preparation of a suspension (10 mL of water per ampoule) at the initial stages of the study. Based on the analogy with the sample of lyophilized L. plantarum cells, it was assumed that the lyophilized bacterial sample differs little in the change of lysis rate from freshly grown cells [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

“…Bacteriolytic activity was determined turbidimetrically by a decrease in absorbance of cell suspension [ 7 , 9 ] at a wavelength of 650 nm and a temperature of 37°C. A change in absorbance (A 650 ) in the range of 5 to 20–30 s from the start of the reaction was used as the initial cell lysis rate.…”

Section: Methodsmentioning

confidence: 99%

“…The activity was measured in a 10 mM buffer solution of MES-Tris-CH 3 COOH at different pH values. As a relative value of activity, values of changes in the initial absorbance -dA/dt (AU/min) are presented, which (with the coefficients for corresponding cells) are proportional to the rate of change in the number of living cells or colony-forming units (-dCFU/dt), proportional to the changes in the lysis rate dΘ/dt (Θ = 0 if all cells remained intact, and Θ = 1 in case of 100% cell lysis) [ 7 , 9 ].…”

Section: Methodsmentioning

confidence: 99%

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Human Interleukin-2 and Hen Egg White Lysozyme: Screening for Bacteriolytic Activity against Various Bacterial Cells

et al. 2016

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The bacteriolytic activity of interleukin-2 and hen egg white lysozyme against 34 different species of microorganisms has been studied. It was found that 6 species of microorganisms are lysed in the presence of interleukin-2. All interleukin-2-sensitive microorganisms belong either to the Enterobacteriaceae, Bacillaceae, or the Lactobacillaceae family. It was also found that 12 species of microorganisms are lysed in the presence of lysozyme, and 16 species of microorganisms are lysed in the presence of sodium dodecyl sulfate (SDS). The bacteriolytic activity of interleukin-2 and lysozyme was studied at various pH values.

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“…To study the thermodynamic parameters of enzyme sorption directly on living bacterial cells, a special measurement technique was developed [13,14], which we use in this work to investigate the effect of amino acids on lysozyme sorption. As model cells, we chose Escherichia coli, which is closely related to various pathogenic bacteria of the Enterobacteriaceae family [15].…”

Section: Introductionmentioning

confidence: 99%

The Adsorption of Lysozyme on Living Escherichia Coli Bacterial Cells in the Presence of Charged Amino Acids and Glycine

et al. 2023

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The effect of glycine, glutamate, aspartate, lysine, histidine, and arginine on the adsorption of lysozyme on living Escherichia coli bacterial cells under conditions close to those for the functioning of the enzyme in saliva was studied. It was shown that the addition of the listed amino acids at concentrations of 1.5 mM for glycine and 5 mM for charged amino acids reduced the desorption constant of lysozyme by 1.4–2.0 times, while the rate of cell lysis (lysozyme activity) increased by 1.4–1.9 times. The maximum amount of lysozyme that can bind to bacteria (adsorption capacity) did not change. An increase in the efficiency of cell lysis in the presence of glycine and charged amino acids can be explained by an increase in the productive adsorption of the enzyme on the surface of bacterial cells.

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Determination of the activity of bacteriolytic enzymes and measurement of their sorption in the system of living cells of Lactobacillus plantarum

Cited by 10 publications

References 15 publications

Bacteriolytic Activity Of Human Interleukin-2, Chicken Egg Lysozyme In The Presence Of Potential Effectors

Bacteriolytic Activity Of Human Interleukin-2, Chicken Egg Lysozyme In The Presence Of Potential Effectors

Human Interleukin-2 and Hen Egg White Lysozyme: Screening for Bacteriolytic Activity against Various Bacterial Cells

The Adsorption of Lysozyme on Living Escherichia Coli Bacterial Cells in the Presence of Charged Amino Acids and Glycine

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