Determination of solanidine in human plasma by radioimmunoassay

Biomarkers of tuber intake

“…Matthew and co-workers made a first report on detection and quantification of human plasma solanidine collected from volunteers in a hospital clinic in the UK [27]. Plasma levels determined by radioimmunoassay ranged from 0.35 to 5.00 ng/ml, and the average level was 1.56 ± 1.17 (17 males) and 1.20 ± 0.93 (27 females) ng/ml.…”

Biomarkers of tuber intake

“…Matthew and co-workers made a first report on detection and quantification of human plasma solanidine collected from volunteers in a hospital clinic in the UK [27]. Plasma levels determined by radioimmunoassay ranged from 0.35 to 5.00 ng/ml, and the average level was 1.56 ± 1.17 (17 males) and 1.20 ± 0.93 (27 females) ng/ml.…”

Effect of feeding solanidine, solasodine and tomatidine to non-pregnant and pregnant mice

“…carbohydrate side-chain from the glycoalkaloids in vivo (Matthew et al, 1983;Harvey et al, 1985aHarvey et al, ,b, 1986Hellenas et al, 1992). It has been detected in human post-mortem liver, suggesting that it is stored in the liver for prolonged periods (Claringbold et al, 1980(Claringbold et al, , 1982.…”

Risk assessment of glycoalkaloids in feed and food, in particular in potatoes and potato‐derived products

“…Solanidine levels in plasma were analysed using the radioimmune assay developed by Matthew et al. ()…”

“…100% cross reactivity was found for solanidine, a-solanine, a-chaconine and desmissidine. A chloroform extraction procedure showed allowed the specific quantification of solanidine Determination of solanidine in plasma Matthew et al (1983) Solanidine 57 volunteers (30 males, age range: 18-44 years old; 27 females, age range: 16-62 years old)…”

“…In the first study, Matthew et al. () described the method and reported the results on 34 human donors. One limitation of this method was that the rabbit immune serum developed for the radioimmunoassay had a 100% cross‐reactivity with α‐solanine, α‐chaconine and demissidine.…”

Biomarkers of tuber intake

“…Matthew and co-workers made a first report on detection and quantification of human plasma solanidine collected from volunteers in a hospital clinic in the UK [27]. Plasma levels determined by radioimmunoassay ranged from 0.35 to 5.00 ng/ml, and the average level was 1.56 ± 1.17 (17 males) and 1.20 ± 0.93 (27 females) ng/ml.…”

Effect of feeding solanidine, solasodine and tomatidine to non-pregnant and pregnant mice

“…carbohydrate side-chain from the glycoalkaloids in vivo (Matthew et al, 1983;Harvey et al, 1985aHarvey et al, ,b, 1986Hellenas et al, 1992). It has been detected in human post-mortem liver, suggesting that it is stored in the liver for prolonged periods (Claringbold et al, 1980(Claringbold et al, , 1982.…”

Risk assessment of glycoalkaloids in feed and food, in particular in potatoes and potato‐derived products

“…Solanidine levels in plasma were analysed using the radioimmune assay developed by Matthew et al. ()…”

“…100% cross reactivity was found for solanidine, a-solanine, a-chaconine and desmissidine. A chloroform extraction procedure showed allowed the specific quantification of solanidine Determination of solanidine in plasma Matthew et al (1983) Solanidine 57 volunteers (30 males, age range: 18-44 years old; 27 females, age range: 16-62 years old)…”

“…In the first study, Matthew et al. () described the method and reported the results on 34 human donors. One limitation of this method was that the rabbit immune serum developed for the radioimmunoassay had a 100% cross‐reactivity with α‐solanine, α‐chaconine and demissidine.…”

Biomarkers of tuber intake

“…Matthew and co-workers made a first report on detection and quantification of human plasma solanidine collected from volunteers in a hospital clinic in the UK [27]. Plasma levels determined by radioimmunoassay ranged from 0.35 to 5.00 ng/ml, and the average level was 1.56 ± 1.17 (17 males) and 1.20 ± 0.93 (27 females) ng/ml.…”

Effect of feeding solanidine, solasodine and tomatidine to non-pregnant and pregnant mice

“…carbohydrate side-chain from the glycoalkaloids in vivo (Matthew et al, 1983;Harvey et al, 1985aHarvey et al, ,b, 1986Hellenas et al, 1992). It has been detected in human post-mortem liver, suggesting that it is stored in the liver for prolonged periods (Claringbold et al, 1980(Claringbold et al, , 1982.…”

Risk assessment of glycoalkaloids in feed and food, in particular in potatoes and potato‐derived products

“…Solanidine levels in plasma were analysed using the radioimmune assay developed by Matthew et al. ()…”

“…100% cross reactivity was found for solanidine, a-solanine, a-chaconine and desmissidine. A chloroform extraction procedure showed allowed the specific quantification of solanidine Determination of solanidine in plasma Matthew et al (1983) Solanidine 57 volunteers (30 males, age range: 18-44 years old; 27 females, age range: 16-62 years old)…”

“…In the first study, Matthew et al. () described the method and reported the results on 34 human donors. One limitation of this method was that the rabbit immune serum developed for the radioimmunoassay had a 100% cross‐reactivity with α‐solanine, α‐chaconine and demissidine.…”