Determination of Serum Tulobuterol Concentrations by Mass Fragmentography: Comparison with an Electron-Capture Gas Chromatographic Method

Matsumura, Kugako; Kubo, Ozora; Sakashita, Toshiko; Kobayashi, Hideo; Watanabe, Keizo; Hirobe, Masaaki

doi:10.1002/jps.2600720526

Cited by 6 publications

(2 citation statements)

References 6 publications

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“…Matsumura et al [5][6][7][8] has developed several gas chromatographic methods coupled with electron capture detector (ECD) or mass spectrometry to determination tulobuterol and its metabolites in human serum and animal urine, the LLOQ of tulobuterol in serum were 0.5 and 1 ng/mL, respectively. Wood et al [9] established a sensitive method for the measurement of tulobuterol in plasma and urine by capillary gas chromatography with ECD and the LLOQ was 0.2 ng/mL.…”

Section: Tulobuterol-hcl {␣-[(Tert-butylamino)methyl]-o-chlo-mentioning

confidence: 99%

“…Liquid-liquid extraction [5][6][7][8][9] was necessary and important because this technique can not only purify but also concentrate the sample. Ethyl acetate, methylene chloride: ethyl acetate mixture (20:80, v/v) and isopropanol: n-hexane (5:95, v/v) were all tested to do extraction and the isopropanol: nhexane mixture (5:95, v/v) was finally adapted because of its high extraction efficiency.…”

Section: Sample Preparationmentioning

confidence: 99%

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High-performance liquid chromatography electrospray ionization mass spectrometry determination of tulobuterol in rabbit's plasma

Zhang

Tian

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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Section: Tulobuterol-hcl {␣-[(Tert-butylamino)methyl]-o-chlo-mentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

High-performance liquid chromatography electrospray ionization mass spectrometry determination of tulobuterol in rabbit's plasma

Zhang

Tian

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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Measurement of tulobuterol in human plasma by capillary gas chromatography and selected ion monitoring detection

Thienpont

Verhaeghe

Leenheer

1987

Biol. Mass Spectrom.

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A method is described for the quantification of tulobuterol in human plasma, based upon selective extraction and high resolution capillary gas chromatography-mass spectrometry. The mass spectrometric determination is based on selected ion monitoring of two fragment ions derived from the N,O-heptafluorobutyryl derivatives of tulobuterol and its internal standard, deschlorotulobuterol. The plasma work-up procedure consists of extraction of 1.0 ml alkalinised plasma with dichloromethane, back-extraction into an acidified aqueous phase, followed by a final extraction with dichloromethane after realkalinisation. The method gave interference-free and linear results. The between-assay variability is 4.7% CV at the 3.0 micrograms l-1 plasma concentration level. The assay permitted quantitative measurements down to 170 ng tulobuterol per litre of plasma and hence provided sufficient sensitivity for quantification of plasma levels after therapeutic doses.

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The pharmacokinetics of the ?2-adrenoceptor agonist, tulobuterol, given transdermally and by inhalation

Uematsu

Nakano

Kosuge

et al. 1993

Eur J Clin Pharmacol

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We have studied the pharmacokinetics of tulobuterol given transdermally or by aerosol inhalation in healthy male volunteers. Tulobuterol was rapidly absorbed after inhalation, with a tmax of 0.8-1.5 h. The Cmax and the AUC increased linearly with dose. Tulobuterol was well absorbed after transdermal administration, with an absorption lag-time of about 4 h. The Cmax and AUC increased linearly with dose and the tmax was about 9-12 h. The mean percentage of drug absorbed during the application of a patch for 24 h was 82-90% after a single dose and 82-85% during repeated dosing. The mean urinary recoveries as unchanged drug after a single inhalation and patch application were 3-4% and 5-6% respectively. Tulobuterol did not accumulate during repeated inhalation or transdermal application. It was well tolerated, except for an increase in heart rate of 10-20 beats.min-1 after five repeated applications of a 4 mg patch.

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Determination of Serum Tulobuterol Concentrations by Mass Fragmentography: Comparison with an Electron-Capture Gas Chromatographic Method

Cited by 6 publications

References 6 publications

High-performance liquid chromatography electrospray ionization mass spectrometry determination of tulobuterol in rabbit's plasma

High-performance liquid chromatography electrospray ionization mass spectrometry determination of tulobuterol in rabbit's plasma

Measurement of tulobuterol in human plasma by capillary gas chromatography and selected ion monitoring detection

The pharmacokinetics of the ?2-adrenoceptor agonist, tulobuterol, given transdermally and by inhalation

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