Determination of polycyclic aromatic hydrocarbons in river waters by blue-cotton adsorption method.

Yamauchi, Aiko; Matsumoto, Noritake; Nakagawa, Hiroyuki; Ohotuka, Takako; Yamazaki, Hiroyasu; Kakiuchi, Yasuo

doi:10.1248/jhs1956.35.283

Cited by 9 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluoranthene and pyrene, found mainly in coal tar, are classified as PAHs. Yamauchi et al (1989) detected a similar peak from the water samples concentrated by blue cotton, using high-performance liquid chromatography. Sayato et al (1993) reported that a mutagenic fraction containing PAHs such as anthracene, pyrene, and BAP was detected in water from the Katsura River.…”

Section: Discussionmentioning

confidence: 84%

Contamination of the Shinano River Water with Mutagenic Substances after the Niigata Chuetsu Earthquake

Fujimoto

Tsuchiya

Shibuya

et al. 2007

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

While normally monitoring the Shinano River water quality, including examinations for mutagenicity, the Niigata Chuetsu Earthquake suddenly occurred on October 23, 2004. However, the influence of this earthquake on the mutagenicity of river water has not yet been well studied. To clarify the regional and seasonal changes in mutagenicity of the Shinano River water, blue rayon was suspended for 24 hrs at 4 sampling sites, once a month from September 2004 through August 2005. Mutagenicity was evaluated by the Ames test using Salmonella typhimurium TA98 (TA98) and TA100 with or without metabolic activation by S9 mixture. To detect and identify poly-aromatic hydrocarbons that may be responsible for the mutagenicity of the river water, we analyzed benzo[a]pyrene, benzophenone, 4-nitrotoluene, or other compounds using gas chromatography-mass spectrometry and total ion chromatogram spectra. Positive manifestations of TA98 with S9 mixture were observed at the 4 sampling sites throughout the 12-month test, showing a tendency to be higher at the downstream site and in winter. However, the highest mutagenicity was observed in the sample collected at the most upstream sampling site in December 2004, and fluoranthene or pyrene consisting mainly in coal tar was detected only in the samples collected in December 2004. Although benzo[a]pyrene, benzophenone, and 4-nitrotoluene were below the detection limits, non-mutagens such as aliphatic hydrocarbons or esters were frequently detected. Our findings indicate that either fluoranthene or pyrene was mainly responsible for the mutagenicity of the river water in December 2004, suggesting the possibility of oil contamination caused by the Niigata Chuetsu Earthquake.the Shinano River water; mutagenicity; the Ames test; gas chromatography-mass spectrometry; total ion chromatogram spectra

show abstract

Section: Discussionmentioning

confidence: 84%

Contamination of the Shinano River Water with Mutagenic Substances after the Niigata Chuetsu Earthquake

Fujimoto

Tsuchiya

Shibuya

et al. 2007

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

show abstract

“…13) Yamauchi et al reported that, although the rate of contribution of hydrophobic PAHs (8 kinds of PAHs) to the mutagenicity of river water concentrate was not calculated, that of hydrophilic PAHs was estimated to be 5%. 2) In this paper, the contribution rates of mutagenicity estimated from analyzed PAH content to the mutagenic magnitude of these water concentrates in TA98 with S9 mix were recognized to be 1.15 and 0.23% in the effluent from a night soil treatment plant and a sewage treatment plant, respectively, and from 0.10% to 0.25% in the river water. The contribution rates of these PAHs were very low, corroborating the report by Yamauchi et al…”

Section: Discussionmentioning

confidence: 87%

“…This river water contains a large amount of trace toxic organic pollutants, because it receives a large quantity of waste water drained from the activities of human life, sewage treatment plants, industrial waste plants, agriculture and road runoff. Many studies have been reported in which municipal river water shows mutagenicity [1][2][3][4][5][6][7][8][9][10][11][12][13][14] and its character is derived from polycyclic aromatic hydrocarbons (PAHs) including nitro-and amino-derivatives. [1][2][3][4][5][6] Recently, WHO established the guidelines for fluoranthene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo (a)pyrene, benzo(ghi)peryrene and indeno (1,2,3-cd)perylene as possible carcinogenic pollution indicators of drinking-water.…”

Section: Introductionmentioning

confidence: 99%

“…These have been used as a preconcentration method in many mutagenicity investigations. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][16][17][18][19][20][21] Yamauchi et al 2) and Sasaki et al 13) used the Blue Cotton hanging method for concentration of organic substances in river water, measured the mutagenicity of concentrate and analyzed 10 kinds of PAHs, Trp-P-1 and Trp-P-2, respectively. Ohe and Nukaya reported the genotoxicity and the content No.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mutagenic Characteristics and Contribution of Polycyclic Aromatic Hydrocarbons to Mutagenicity of Concentrates from Municipal River Water by Blue Chitin Column.

Nagai¹,

Kano²,

Funasaka³

et al. 2002

JOURNAL OF HEALTH SCIENCE

View full text Add to dashboard Cite

The mutagenic characteristics of concentrates from environmental water such as the effluent from various treatment plants and river waters using a modified Blue Chitin column method were determined by Ames assay with Salmonella typhimurium TA98, YG1021 and YG1024. This was done to estimate the quantitative contribution rate of mutagenicity estimated from analyzed polycyclic aromatic hydrocarbons (PAHs) content in the same concentrates to the mutagenicity of environmental water. Moreover, PAHs content and the mutagenicity of the river sediment-extract were measured to elucidate the fate of PAHs in the river. Many kinds of environmental waters and river sediment possessed principally indirect frame-shift type mutagenicity. Concentrates from many kinds of environmental waters possibly contained aminoarenes assayed with YG1021 and YG1024. In many kinds of environmental waters, the contribution rates of mutagenic magnitude estimated from seventeen analyzed PAH contents to the mutagenic magnitude of these water concentrates in TA98 with S9 mix were recognized to be from 0.10 to 1.15%. However, the contribution rate of mutagenic magnitude estimated from ten analyzed PAHs contents to the mutagenic magnitude of the Arata River sediment extract in TA98 with S9 mix was 64.2%. The high concentration of PAHs in the river sediment suggested that hydrophobic PAHs in the water might easily accumulate in the river sediment after adsorbing to the suspended solid.

show abstract

“…It adsorbs compounds with three or more fused rings. Although this method does not yield quantitative results, it is a simple method for extracting chemical compounds and can be applied to a greater number of samples for river water pollution tests than other methods (8).…”

Section: Introductionmentioning

confidence: 99%

Validity of Mutagenic Activity as an Indicator of River Water Pollution

Tsukatani

Tanaka

Sera

et al. 2003

Environ. Health Prev. Med.

View full text Add to dashboard Cite

Objectives: To investigate if mutagenicity could be expressed by known water pollution indicators, we determined the mutagenic activity of blue rayon extracts from sampled river water with the Ames test utilizing new strains of bacteria, and compared the results with those of known indicators of water pollution.Methods: Water samples were collected by the blue rayon adsorption method at sixteen sites in six rivers in the North Kyushu district. The Assay of mutagenicity was carried out using the Ames test. The test strains were Salmonella typhimurium TA100, YG1024, YG1041 and YG1042. B(a)P, Trp-P-1 and Trp-P-2 were quantified by HPLC. Determinations of SS, BOD, COD, T-N, T-P, DOC, and A 260 / DOC were performed.Results: The extracts from five sampling sites showed higher mutagenicity toward strain YG1024 with or without S9mix, and the extracts from two of these five sites showed higher mutagenicity toward strain YG1041 with and without S9mix. However, the water pollution indicators did not show specific trends that were consistent with the mutagenic activity.Conclusions: Since the mutagenic activity of river water could not be predicted using known water pollution indicators, we recommend that biological examinations such as mutagenicity tests be added to the indicators that are currently in use.

show abstract

Determination of polycyclic aromatic hydrocarbons in river waters by blue-cotton adsorption method.

Cited by 9 publications

References 0 publications

Contamination of the Shinano River Water with Mutagenic Substances after the Niigata Chuetsu Earthquake

Contamination of the Shinano River Water with Mutagenic Substances after the Niigata Chuetsu Earthquake

Mutagenic Characteristics and Contribution of Polycyclic Aromatic Hydrocarbons to Mutagenicity of Concentrates from Municipal River Water by Blue Chitin Column.

Validity of Mutagenic Activity as an Indicator of River Water Pollution

Contact Info

Product

Resources

About