Determination of Piperacillin/Tazobactam and Ticarcillin/Clavulanate Susceptibilities in Pseudomonas aeruginosa Isolates in Hospitalised Patients by E-test Gradient Method and Comparison of Results with Disk Diffusion Tests

Direkel, Sahin; Uzunoğlu, Emel; Uzalp, Cagla; Findik, Ezgi; Tontak, Said; Ahmadli, Cengiz

doi:10.4172/2327-5073.1000273

Cited by 5 publications

(6 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lowest antibiotic resistance rates in biofilm-positive and biofilm-negative isolates were seen against piperacillin/tazobactam (34.3 and 15.4%, respectively). This result agrees with Namuq et al [ 29 ] who reported piperacillin/tazobactam resistance (5%), Hoque et al [ 30 ] who reported (3.37%), and Direkel et al [ 31 ] who reported (7%). Also, in a study performed by Delissalde and Amábile-Cuevas, the resistance of biofilm-producing strains against piperacillin/tazobactam was 35%, which was very similar to our results [ 28 ].…”

Section: Discussionsupporting

confidence: 92%

Prevalence of algD, pslD, pelF, Ppgl, and PAPI-1 Genes Involved in Biofilm Formation in Clinical Pseudomonas aeruginosa Strains

Rajabi

Salimizand

Khodabandehloo

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

Introduction. Biofilm formation is one of the main virulence factors in Pseudomonas aeruginosa infections. This study is aimed at investigating the presence of genes involved in biofilm formation in clinical P. aeruginosa isolates. Material and Methods. A cross-sectional study was conducted on 112 P. aeruginosa isolates. The biofilm formation assay was performed on all isolates. Antimicrobial resistance was determined by the disk diffusion method, and the presence of genes was detected by polymerase chain reaction. Isolates were typed with Rep-PCR. Results. The results of biofilm formation demonstrated that 85 strains (75.9%) were biofilm producers, and 27 strains (24.1%) were nonproducer isolates. Antibiotic susceptibility pattern in biofilm-positive and biofilm-negative isolates obtained from hospitalized patients showed a high rate of antibiotic resistance to amoxicillin with 95.7% and 92.3%, respectively. Based on PCR amplification results, the frequency of genes involved in biofilm formation among all isolates was as follows: algD (78.6%), pelF (70.5%), pslD (36.6%), Ppgl (0%), and PAPI-1 (77.6%). Rep-PCR typing demonstrated that 112 P. aeruginosa isolates were classified into 57 types according to 70% cut-off. The predominant type was A which contained 15 isolates. Moreover, 7 isolates were clustered in genotype B, followed by C type (6), D (4), E (4), F (4), G (4), H (3), I (3), J (3 isolates), and 12 genotypes, each containing two isolates. Also, 35 isolates were distributed in scattered patterns and showed single types. Conclusion. Study results showed significant association between biofilm formation and resistance to antibiotics such as ceftazidime and meropenem. Analysis of Rep-PCR patterns indicated that the evaluated isolates were heterogeneous, relatively.

show abstract

Section: Discussionsupporting

confidence: 92%

Prevalence of algD, pslD, pelF, Ppgl, and PAPI-1 Genes Involved in Biofilm Formation in Clinical Pseudomonas aeruginosa Strains

Rajabi

Salimizand

Khodabandehloo

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…P. aeruginosa (PA1) was resistant to piperacillin, piperacillin/tazobactam, meropenem and ceftazidime. Piperacillin, cefotaxime and ceftazidime resistant E.coli isolates and piperacillin/tazobactam, meropenem and ceftazidime resistant P. aeruginosa isolates have been reported in previos studies (Tavío et al, 2014;Hunter et al, 2010;Akova, 2016;Andersen et al, 2005;Direkel et al, 2017;Mirsalehian et al, 2017;Rostami et al, 2018;Kos et al, 2016). These isolates restricts treatment options and cause problems in clinical settings.…”

Section: Resarch Findingsmentioning

confidence: 99%

“…In a study conducted in our country, it has been reported that piperacillin-tazobactam and amikacin are the most effective antibiotics against P. aeruginosa isolates. However, piperacillintazobactam resistance rates in P. aeruginosa isolates increased between 2007 and 2015 (Direkel et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Seçilen Bitkilerin DMSO Özütlerinin Antibiyotik Dirençli Klinik İzolatlara Karşı Antibakteriyel Aktivitesi

Saral¹,

Kardil²,

Düzgün³

2019

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi

View full text Add to dashboard Cite

In this study, we aimed to find out new herbal materials that are able to inhibit the growth of the P. aeruginosa and E.coli clinical isolates that has antibiotic resistance. Clinical isolates used in this research are E. coli (n=1) and P.aeruginosa (n=1). Antibiotic susceptibility profiles of E. coli and P. aeruginosa were determined using e-test. Plants were collected in Trabzon region of Turkey are Calendula officinalis, Hypericum perforatum and Glycyrrhiza glabra. DMSO were used as solvent and solid-liquid extraction was employed. Micro-dilution method was preferred fo the determination of the minimum inhibitory concentration (MIC). MIC results were obtained through observation of turbidities. According to E-test results, while P. aeruginosa was resistant to piperacillin, piperacillin/tazobactam, meropenem and ceftazidime, E. coli was resistant to piperacillin, cefotaxime and ceftazidime. DMSO extract of Calendula officinalis showed very strong activity against PA1 with the best MIC (5 mg/mL). DMSO extract of three plant had lower MIC values (5-10 mg/ml) for EC1 and PA1 than ampicillin. In future studies antibacterial activity of different solvents extracts of these plants and other plants against antibiotic resistant clinical isolates will be examined. Natural products from plants are promising in fighting with antibiotic-resistant bacteria.

show abstract

“…The antibiotic penetration from the strip into the medium containing agar caused an oval zone. Moreover, the MIC was decided to be the point where the oval zone crossed the strip (21,22).…”

Section: Resultsmentioning

confidence: 99%

Determination of antibiotic resistance and minimum inhibitory concentration of meropenem and imipenem growth in Klebsiella strains isolated from urinary tract infection in Shahrekord educational hospitals

Kakian

Zamzad

Gholipour

et al. 2019

J Shahrekord Univ Med Sci

View full text Add to dashboard Cite

Background and aims: Klebsiella is an opportunistic organism that is the cause of severe diseases such as pneumonia, septicemia, and urinary tract infections (UTIs). In addition, high antibiotic resistance has challenged the treatment of this bacterium. However, carbapenem antibiotics are considered as the therapeutic agents for selecting the treatment of penicillin- and cephalosporin-resistant gram-negative bacterial infections. The present study aimed to determine the resistance and minimum inhibitory concentration (MIC) of meropenem and imipenem. Methods: A total of 80 Klebsiella spp isolated from UTIs were collected in various educational wards (i.e., urology, obstetrics, and gynecology, as well as the units of infectious diseases, internal medicine, and intensive care) in different hospitals of Shahrekord. The isolates were then identified by using biochemical tests. Further, disc diffusion method was employed to determine the antibiotic resistance. Furthermore, MIC was estimated by the Epsilon-test strip. Moreover, P=Q=0.50, an error of 0.05, and an accuracy of 0.11 were considered for determining the sample size (n=80). Results: Based on the results of disc diffusion method, 24 strains were resistant to meropenem and imipenem. Additionally, the MIC was 24 (30%) by the E-test. In addition, 24 isolates had a MIC of ≥4 μg/mL for meropenem and imipenem and thus were resistant while 18 isolates were found to have a MIC of 1≤ MIC<4 μg/mL and therefore, were considered semi-sensitive (P<0.001). Conclusion: In general, Klebsiella strains were found to be resistant to meropenem and imipenem. Therefore, rapid and accurate identification of these strains and the selection of appropriate antibiotics can help quickly eradicate the infections caused by these bacteria. Accordingly, a waste of time, the consumption of medication, or even an increased resistance are prevented.

show abstract

Determination of Piperacillin/Tazobactam and Ticarcillin/Clavulanate Susceptibilities in Pseudomonas aeruginosa Isolates in Hospitalised Patients by E-test Gradient Method and Comparison of Results with Disk Diffusion Tests

Cited by 5 publications

References 16 publications

Prevalence of algD, pslD, pelF, Ppgl, and PAPI-1 Genes Involved in Biofilm Formation in Clinical Pseudomonas aeruginosa Strains

Prevalence of algD, pslD, pelF, Ppgl, and PAPI-1 Genes Involved in Biofilm Formation in Clinical Pseudomonas aeruginosa Strains

Seçilen Bitkilerin DMSO Özütlerinin Antibiyotik Dirençli Klinik İzolatlara Karşı Antibakteriyel Aktivitesi

Determination of antibiotic resistance and minimum inhibitory concentration of meropenem and imipenem growth in Klebsiella strains isolated from urinary tract infection in Shahrekord educational hospitals

Contact Info

Product

Resources

About